RegulonDB RegulonDB 10.7: Gene Form
   

prfB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

recJ lysS prfB TSS_3165 TSS_3165 TSS_3164 TSS_3164 TSS_3163 TSS_3163 TSS_3162 (cluster) TSS_3162 (cluster) TSS_3161 TSS_3161 TSS_3160 TSS_3160 TSS_3159 TSS_3159 TSS_3158 TSS_3158 TSS_3157 TSS_3157 TSS_3156 TSS_3156 TSS_3155 TSS_3155 TSS_3154 TSS_3154 TSS_3153 TSS_3153 TSS_3152 TSS_3152 TSS_3151 TSS_3151 TSS_3150 TSS_3150 TSS_3149 TSS_3149 TSS_3148 TSS_3148 TSS_3147 TSS_3147 TSS_3146 TSS_3146

Gene      
Name: prfB    Texpresso search in the literature
Synonym(s): ECK2886, EG10762, b2891, supK
Genome position(nucleotides): 3035184 <-- 3036282 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.5
External database links:  
ASAP:
ABE-0009488
CGSC:
17635
ECHOBASE:
EB0755
OU-MICROARRAY:
b2891
PORTECO:
prfB
REGULONDB:
b2891
STRING:
511145.b2891
M3D: prfB
COLOMBOS: prfB
PortEco: b2891


Product      
Name: peptide chain release factor RF2
Synonym(s): PrfB, RF2, SupK
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 41.251
Isoelectric point: 4.38
Motif(s):
 
Type Positions Sequence
221 -> 223 FVY
228 -> 337 DDIDIEINPADLRIDVYRTSGAGGQHVNRTESAVRITHIPTGIVTQCQNDRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIKDLR
246 -> 246 T
322 -> 323 QI
252 -> 252 Q

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.2 - translation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003747 - translation release factor activity
GO:0016149 - translation release factor activity, codon specific
biological_process GO:0006412 - translation
GO:0006415 - translational termination
Note(s): Note(s): ...[more].
Evidence: [APPH] Assay of protein purified to homogeneity
Reference(s): [1] Zaher HS., et al., 2009
External database links:  
DIP:
DIP-10559N
ECOCYC:
EG10762-MONOMER
ECOLIWIKI:
b2891
INTERPRO:
IPR004374
INTERPRO:
IPR000352
INTERPRO:
IPR005139
MINT:
MINT-1238444
MODBASE:
P07012
PANTHER:
PTHR11075:SF6
PDB:
5U4J
PDB:
5U9F
PDB:
5U9G
PDB:
6C4H
PDB:
6C4I
PDB:
5U4I
PDB:
5MGP
PDB:
5MDW
PDB:
5MDV
PDB:
5H5U
PDB:
5DFE
PDB:
5CZP
PDB:
1ML5
PDB:
1MI6
PDB:
1GQE
PFAM:
PF03462
PFAM:
PF00472
PRIDE:
P07012
PRODB:
PRO_000023589
PROSITE:
PS00745
PROTEINMODELPORTAL:
P07012
REFSEQ:
NP_417367
SMART:
SM00937
SMR:
P07012
UNIPROT:
P07012


Operon      
Name: xerD-dsbC-recJ-prfB-lysS         
Operon arrangement:
Transcription unit        Promoter
dsbC
dsbC-recJ-prfB
xerD-dsbC-recJ
prfB-lysS
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3146 3034170 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3147 3034200 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3148 3034309 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3149 3034602 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3150 3034638 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3151 3034998 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3152 3035019 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3153 3035030 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3154 3035058 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3155 3035062 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3156 3035121 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3157 3035166 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3158 3035202 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3159 3035315 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3160 3035434 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3161 3036095 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3162 (cluster) 3036210 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_3163 3036221 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3164 3036252 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_3165 3036324 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Zaher HS., Green R., 2009, Quality control by the ribosome following peptide bond formation., Nature 457(7226):161-6

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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