RegulonDB RegulonDB 10.8: Gene Form
   

proS gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

proS yaeF trmO rcsF terminator proSp proSp TSS_397 TSS_397 yaeFp1 yaeFp1

Gene      
Name: proS    Texpresso search in the literature
Synonym(s): ECK0194, EG10770, b0194, drp, drpA
Genome position(nucleotides): 217057 <-- 218775 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.51
External database links:  
ASAP:
 ABE-0000657
CGSC:
 360
ECHOBASE:
 EB0763
OU-MICROARRAY:
 b0194
PortEco:
 proS
STRING:
 511145.b0194
COLOMBOS: proS


Product      
Name: proline—tRNA ligase
Synonym(s): Drp, DrpA, ProRS, ProS
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 63.693
Isoelectric point: 4.876
Motif(s):
 
Type Positions Sequence
279 -> 279 K
386 -> 386 D
95 -> 459 RFVDRGERPFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPQVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVAGDPSPDGQGRLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQN
394 -> 394 D
26 -> 27 ML

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.1 - amino acid -activation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016874 - ligase activity
GO:0004812 - aminoacyl-tRNA ligase activity
GO:0004827 - proline-tRNA ligase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0002161 - aminoacyl-tRNA editing activity
GO:0043906 - Ala-tRNA(Pro) hydrolase activity
biological_process GO:0106074 - aminoacyl-tRNA metabolism involved in translational fidelity
GO:0006412 - translation
GO:0006418 - tRNA aminoacylation for protein translation
GO:0006433 - prolyl-tRNA aminoacylation
Note(s): Note(s): ...[more].
Reference(s): [1] Kumar S., et al., 2012
External database links:  
DIP:
 DIP-10573N
ECOCYC:
 PROS-MONOMER
ECOLIWIKI:
 b0194
INTERPRO:
 IPR007214
INTERPRO:
 IPR036754
INTERPRO:
 IPR036621
INTERPRO:
 IPR033730
INTERPRO:
 IPR023717
INTERPRO:
 IPR006195
INTERPRO:
 IPR004500
INTERPRO:
 IPR004154
INTERPRO:
 IPR002316
INTERPRO:
 IPR002314
MINT:
 MINT-1261583
MODBASE:
 P16659
PANTHER:
 PTHR11451:SF3
PFAM:
 PF00587
PFAM:
 PF03129
PFAM:
 PF04073
PRIDE:
 P16659
PRINTS:
 PR01046
PRODB:
 PRO_000023601
PROSITE:
 PS50862
REFSEQ:
 NP_414736
SMR:
 P16659
UNIPROT:
 P16659


Operon      
Name: proS         
Operon arrangement:
Transcription unit        Promoter
proS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yaeFp1 217135 reverse Similarity to the consensus
Read more > 		[ICWHO] [2]
  promoter TSS_397 218831 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Kumar S., Das M., Hadad CM., Musier-Forsyth K., 2012, Substrate and enzyme functional groups contribute to translational quality control by bacterial prolyl-tRNA synthetase., J Phys Chem B 116(23):6991-9

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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