RegulonDB RegulonDB 10.8: Gene Form
   

purA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

purA nsrR yjeT PurR MarA PurR TSS_5047 (cluster) TSS_5047 (cluster) TSS_5046 TSS_5046 nsrRp nsrRp TSS_5045 TSS_5045 TSS_5044 TSS_5044 TSS_5043 TSS_5043 TSS_5042 TSS_5042 TSS_5041 TSS_5041 TSS_5040 TSS_5040 TSS_5039 (cluster) TSS_5039 (cluster) TSS_5038 TSS_5038 TSS_5037 TSS_5037 TSS_5036 TSS_5036 TSS_5035 TSS_5035 TSS_5034 TSS_5034 TSS_5033 TSS_5033 TSS_5032 (cluster) TSS_5032 (cluster) TSS_5031 TSS_5031 TSS_5030 (cluster) TSS_5030 (cluster) TSS_5029 TSS_5029 TSS_5028 TSS_5028 purAp purAp

Gene      
Name: purA    Texpresso search in the literature
Synonym(s): ECK4173, EG10790, adeK, b4177
Genome position(nucleotides): 4404687 --> 4405985 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.89
External database links:  
ASAP:
ABE-0013672
CGSC:
345
ECHOBASE:
EB0783
MIM:
617030
OU-MICROARRAY:
b4177
PortEco:
purA
STRING:
511145.b4177
COLOMBOS: purA


Product      
Name: adenylosuccinate synthetase
Synonym(s): AdeK, PurA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 47.345
Isoelectric point: 5.13
Motif(s):
 
Type Positions Sequence
13 -> 19 GDEGKGK
417 -> 417 G
2 -> 432 GNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSEACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAEKLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDFVMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGAGPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLDVLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGLPQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA
18 -> 18 G
300 -> 306 ATTGRRR

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.1 - purine biosynthesis
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.33 - nucleotide and nucleoside conversions
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0004019 - adenylosuccinate synthase activity
GO:0000166 - nucleotide binding
GO:0005525 - GTP binding
GO:0000287 - magnesium ion binding
GO:0042803 - protein homodimerization activity
biological_process GO:0006164 - purine nucleotide biosynthetic process
GO:0006974 - cellular response to DNA damage stimulus
GO:0015949 - nucleobase-containing small molecule interconversion
GO:0046040 - IMP metabolic process
GO:0046086 - adenosine biosynthetic process
GO:0044208 - 'de novo' AMP biosynthetic process
External database links:  
DIP:
DIP-36219N
ECOCYC:
ADENYLOSUCCINATE-SYN-MONOMER
ECOLIWIKI:
b4177
INTERPRO:
IPR042110
INTERPRO:
IPR042111
INTERPRO:
IPR042109
INTERPRO:
IPR033128
INTERPRO:
IPR027417
INTERPRO:
IPR018220
INTERPRO:
IPR001114
MINT:
MINT-1286548
PANTHER:
PTHR11846
PDB:
1GIN
PDB:
1HON
PDB:
1HOO
PDB:
1HOP
PDB:
1JUY
PDB:
1KJX
PDB:
1KKB
PDB:
1KKF
PDB:
1KSZ
PDB:
1NHT
PDB:
1QF4
PDB:
1QF5
PDB:
1SON
PDB:
1SOO
PDB:
2GCQ
PDB:
1GIM
PDB:
1CH8
PDB:
1CIB
PDB:
1CG4
PDB:
1CG3
PDB:
1CG1
PDB:
1CG0
PDB:
1ADI
PDB:
1ADE
PFAM:
PF00709
PRIDE:
P0A7D4
PRODB:
PRO_000023637
PROSITE:
PS00513
PROSITE:
PS01266
REFSEQ:
NP_418598
SMART:
SM00788
SMR:
P0A7D4
UNIPROT:
P0A7D4


Operon      
Name: purA         
Operon arrangement:
Transcription unit        Promoter
purA


Transcriptional Regulation      
Display Regulation             
Activated by: GadE
Repressed by: MarA, PurR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_5028 4404678 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5029 4404684 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5030 (cluster) 4404691 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_5031 4404694 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5032 (cluster) 4404734 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_5033 4404739 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5034 4404742 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_5035 4404745 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5036 4404753 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5037 4404830 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5038 4404833 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5039 (cluster) 4404835 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_5040 4404848 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5041 4404852 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5042 4405214 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5043 4405777 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5044 4405781 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5045 4405785 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5046 4406415 forward nd [RS-EPT-CBR] [1]
  promoter TSS_5047 (cluster) 4406597 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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