RegulonDB

RegulonDB RegulonDB 10.8: Gene Form

purC gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	purC
Synonym(s):	ECK2472, EG10791, ade, ade(g), b2476
Genome position(nucleotides):	2596905 <-- 2597618 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.24
Reference(s):	[1] Parker J. 1984 [2] Tiedeman AA., et al., 1990
External database links:

ASAP:	ABE-0008156
CGSC:	343
ECHOBASE:	EB0784
OU-MICROARRAY:	b2476
PortEco:	purC
STRING:	511145.b2476
COLOMBOS:	purC

Product

Name:

phosphoribosylaminoimidazole-succinocarboxamide synthase

Synonym(s):

Ade, PurC

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

26.995

Isoelectric point:

4.787

Motif(s):

Type	Positions	Sequence
	7 -> 231	LYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFDRKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLVKRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKANDVLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSLGGLIEAYEAVARR

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.1 - purine biosynthesis
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0016874 - ligase activity GO:0000166 - nucleotide binding GO:0005524 - ATP binding GO:0004639 - phosphoribosylaminoimidazolesuccinocarboxamide synthase activity GO:0042802 - identical protein binding
	biological_process	GO:0006164 - purine nucleotide biosynthetic process GO:0009236 - cobalamin biosynthetic process GO:0006189 - 'de novo' IMP biosynthetic process

External database links:

DIP:

ECOCYC:

SAICARSYN-MONOMER

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MINT:

MODBASE:

PANTHER:

PDB:

PDB:

PFAM:

PRIDE:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMR:

SWISSMODEL:

UNIPROT:

Operon

Name:

purC

Operon arrangement:

Transcription unit	Promoter
purC	purCp

Transcriptional Regulation
	Display Regulation
Repressed by:	PurR

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	tmcAp5	2595938		reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 7.853. P-value: 2.045e-6 Read more >	[ICWHO]	[3]
	promoter	TSS_2766	2596763		reverse	nd	[RS-EPT-CBR]	[4]
	promoter	TSS_2767	2597654		reverse	nd	[RS-EPT-CBR]	[4]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Parker J., 1984, Identification of the purC gene product of Escherichia coli., J Bacteriol 157(3):712-7
[2] Tiedeman AA., DeMarini DJ., Parker J., Smith JM., 1990, DNA sequence of the purC gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12., J Bacteriol 172(10):6035-41
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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