RegulonDB RegulonDB 10.8: Gene Form
   

purF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

purF ubiX cvpA terminator

Gene      
Name: purF    Texpresso search in the literature
Synonym(s): ECK2306, EG10794, ade, ade(ub), b2312, purC
Genome position(nucleotides): 2428721 <-- 2430238 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.9
Reference(s): [1] Makaroff CA., et al., 1985
[2] Rolfes RJ., et al., 1988
[3] Sampei G., et al., 1988
[4] Tso JY., et al., 1982
External database links:  
ASAP:
 ABE-0007631
CGSC:
 340
ECHOBASE:
 EB0787
OU-MICROARRAY:
 b2312
PortEco:
 purF
STRING:
 511145.b2312
COLOMBOS: purF


Product      
Name: amidophosphoribosyltransferase
Synonym(s): Ade, PurC, PurF
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 56.488
Isoelectric point: 5.181
Motif(s):
 
Type Positions Sequence
50 -> 50 G
2 -> 236 CGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFRLRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHNGNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAATNRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLGFDFLRDVAPGEAIYITEE
45 -> 46 LR
337 -> 337 M
386 -> 386 A

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.1 - purine biosynthesis
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016757 - transferase activity, transferring glycosyl groups
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0004044 - amidophosphoribosyltransferase activity
GO:0000287 - magnesium ion binding
GO:0042802 - identical protein binding
biological_process GO:0006164 - purine nucleotide biosynthetic process
GO:0006541 - glutamine metabolic process
GO:0009116 - nucleoside metabolic process
GO:0006189 - 'de novo' IMP biosynthetic process
GO:0009113 - purine nucleobase biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
DISPROT:
 DP00578
ECOCYC:
 PRPPAMIDOTRANS-MONOMER
ECOLIWIKI:
 b2312
INTERPRO:
 IPR029055
INTERPRO:
 IPR035584
INTERPRO:
 IPR000836
INTERPRO:
 IPR005854
INTERPRO:
 IPR017932
INTERPRO:
 IPR029057
MODBASE:
 P0AG16
PDB:
 6CZF
PDB:
 1ECG
PDB:
 1ECF
PDB:
 1ECC
PDB:
 1ECB
PDB:
 1ECJ
PFAM:
 PF00156
PRIDE:
 P0AG16
PROSITE:
 PS00103
PROSITE:
 PS51278
REFSEQ:
 NP_416815
SMR:
 P0AG16
SWISSMODEL:
 P0AG16
UNIPROT:
 P0AG16


Operon      
Name: cvpA-purF-ubiX         
Operon arrangement:
Transcription unit        Promoter
cvpA-purF-ubiX
cvpA-purF-ubiX
ubiX


Transcriptional Regulation      
Display Regulation             
Repressed by: PurR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Makaroff CA., Zalkin H., 1985, Regulation of Escherichia coli purF. Analysis of the control region of a pur regulon gene., J Biol Chem 260(18):10378-87

 [2] Rolfes RJ., Zalkin H., 1988, Regulation of Escherichia coli purF. Mutations that define the promoter, operator, and purine repressor gene., J Biol Chem 263(36):19649-52

 [3] Sampei G., Mizobuchi K., 1988, Nucleotide sequence of the Escherichia coli purF gene encoding amidophosphoribosyltransferase for de novo purine nucleotide synthesis., Nucleic Acids Res 16(17):8717

 [4] Tso JY., Zalkin H., van Cleemput M., Yanofsky C., Smith JM., 1982, Nucleotide sequence of Escherichia coli purF and deduced amino acid sequence of glutamine phosphoribosylpyrophosphate amidotransferase., J Biol Chem 257(7):3525-31

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