Gene
Name:	purN
Synonym(s):	ECK2496, EG10799, ade, ade(c), b2500
Genome position(nucleotides):	2622234 --> 2622872 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.68
Reference(s):	[1] Andersen PS., et al., 1992 [2] Smith JM., et al., 1987 [3] Watanabe W., et al., 1989
External database links:

ASAP:	ABE-0008232
CGSC:	17623
ECHOBASE:	EB0792
OU-MICROARRAY:	b2500
PortEco:	purN
STRING:	511145.b2500
COLOMBOS:	purN

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2780	2620986	reverse	nd	[RS-EPT-CBR]	[5]
promoter	ppkp5	2622874	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 7.446. P-value: 3.144e-6 Read more >	[ICWHO]	[6]
promoter	ppkp6	2622990	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 7.65. P-value: 2.353e-6 Read more >	[ICWHO]	[6]
promoter	TSS_2781 (cluster)	2625007	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2625007 to 2625008, where the highest frequency is at position 2623030. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[5]
promoter	TSS_2782 (cluster)	2625014	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2625014 to 2625015, where the highest frequency is at position 2623036. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[5]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Andersen PS., Smith JM., Mygind B., 1992, Characterization of the upp gene encoding uracil phosphoribosyltransferase of Escherichia coli K12., Eur J Biochem 204(1):51-6
[2] Smith JM., Daum HA., 1987, Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12., J Biol Chem 262(22):10565-9
[3] Watanabe W., Sampei G., Aiba A., Mizobuchi K., 1989, Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis., J Bacteriol 171(1):198-204
[4] Morikis D., Elcock AH., Jennings PA., McCammon JA., 2001, Proton transfer dynamics of GART: the pH-dependent catalytic mechanism examined by electrostatic calculations., Protein Sci 10(11):2379-92
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Transcriptional Regulation
	Display Regulation
Repressed by:	PurR, FNR