RegulonDB RegulonDB 11.0: Gene Form
   

putP gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

putP efeO efeU ymdG CpxR CRP CRP terminator efeOp1 efeOp1 efeOp2 efeOp2 efeUp efeUp putPp5 putPp5 TSS_1317 TSS_1317 putPp4 putPp4 putPp3 putPp3 putPp2 putPp2 putPp1 putPp1

Gene      
Name: putP    Texpresso search in the literature
Synonym(s): ECK1006, EG10802, b1015, putC
Genome position(nucleotides): 1079305 --> 1080813
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.68
External database links:  
ASAP:
 ABE-0003433
CGSC:
 332
ECHOBASE:
 EB0795
ECOLIHUB:
 putP
OU-MICROARRAY:
 b1015
STRING:
 511145.b1015
COLOMBOS: putP


Shine dalgarno      
Sequence: aataatttttTGGAGActttagATG


Product      
Name: proline:Na+ symporter
Synonym(s): PPI, PutC, PutP, PutP proline SSS transporter, proline permease I
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 54.344
Isoelectric point: 8.668
Motif(s):
 
Type Positions Sequence Comment
4 -> 26 STPMLVTFCVYIFGMILIGFIAW UniProt: Helical.
27 -> 66 RSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPG UniProt: Hydrophilic; Sequence Annotation Type: region of interest.
35 -> 437 YILGGRSLGPFVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLAGAVAVGFFGIAYFNDHPALAGAVNQNAERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIG
67 -> 87 AVFLSGISESWIAIGLTLGAW UniProt: Helical.
88 -> 124 INWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRI UniProt: Hydrophilic; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015293 - symporter activity
GO:0022857 - transmembrane transporter activity
GO:0005298 - proline:sodium symporter activity
GO:0031402 - sodium ion binding
biological_process GO:0006811 - ion transport
GO:0006865 - amino acid transport
GO:0015824 - proline transport
GO:0006814 - sodium ion transport
GO:0015912 - short-chain fatty acid transport
GO:0055085 - transmembrane transport
GO:0003333 - amino acid transmembrane transport
GO:1903825 - organic acid transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Pirch T., et al., 2003
[2] Quick M., et al., 1997
[3] Quick M., et al., 1998
[4] Quick M., et al., 1999
External database links:  
ALPHAFOLD:
 P07117
ECOCYC:
 PUTP-MONOMER
ECOLIWIKI:
 b1015
INTERPRO:
 IPR001734
INTERPRO:
 IPR011851
INTERPRO:
 IPR038377
INTERPRO:
 IPR018212
PFAM:
 PF00474
PRIDE:
 P07117
PRODB:
 PRO_000023648
PROSITE:
 PS50283
PROSITE:
 PS00457
PROSITE:
 PS00456
REFSEQ:
 NP_415535
UNIPROT:
 P07117


Operon      
Name: putP         
Operon arrangement:
Transcription unit        Promoter
putP
putP
putP
putP
putP


Transcriptional Regulation      
Display Regulation             
Repressed by: CRP, PutA


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1317 1079288 forward nd [RS-EPT-CBR] [5]
  promoter efeOp2 1082154 forward nd [ICWHO] [6]
  promoter efeOp1 1082243 forward nd [ICWHO] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Pirch T., Landmeier S., Jung H., 2003, Transmembrane domain II of the Na+/proline transporter PutP of Escherichia coli forms part of a conformationally flexible, cytoplasmic exposed aqueous cavity within the membrane., J Biol Chem 278(44):42942-9

 [2] Quick M., Jung H., 1997, Aspartate 55 in the Na+/proline permease of Escherichia coli is essential for Na+-coupled proline uptake., Biochemistry 36(15):4631-6

 [3] Quick M., Jung H., 1998, A conserved aspartate residue, Asp187, is important for Na+-dependent proline binding and transport by the Na+/proline transporter of Escherichia coli., Biochemistry 37(39):13800-6

 [4] Quick M., Stolting S., Jung H., 1999, Role of conserved Arg40 and Arg117 in the Na+/proline transporter of Escherichia coli., Biochemistry 38(41):13523-9

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

RegulonDB