RegulonDB

Gene
Name:	putP
Synonym(s):	ECK1006, EG10802, b1015, putC
Genome position(nucleotides):	1079305 --> 1080813 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.68
External database links:

ASAP:	ABE-0003433
CGSC:	332
ECHOBASE:	EB0795
OU-MICROARRAY:	b1015
PortEco:	putP
STRING:	511145.b1015
COLOMBOS:	putP

Shine dalgarno
Sequence:	aataatttttTGGAGActttagATG

Product

Name:

proline:Na⁺ symporter

Synonym(s):

PPI, PutC, PutP, PutP proline SSS transporter, proline permease I

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

54.344

Isoelectric point:

8.668

Motif(s):

Type	Positions	Sequence
	35 -> 437	YILGGRSLGPFVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLAGAVAVGFFGIAYFNDHPALAGAVNQNAERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIG
	163 -> 184	ALWAGAAATILYTFIGGFLAVS
	476 -> 502	PSAAMQKRFAEADAHYHSAPPSRLQES
	67 -> 87	AVFLSGISESWIAIGLTLGAW
	125 -> 150	LRIISALVILLFFTIYCASGIVAGAR

Type	Positions	Sequence
	35 -> 437	YILGGRSLGPFVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLAGAVAVGFFGIAYFNDHPALAGAVNQNAERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIG
	163 -> 184	ALWAGAAATILYTFIGGFLAVS
	476 -> 502	PSAAMQKRFAEADAHYHSAPPSRLQES
	67 -> 87	AVFLSGISESWIAIGLTLGAW
	125 -> 150	LRIISALVILLFFTIYCASGIVAGAR
	320 -> 340	ILFNPWIAGILLSAILAAVMS
	371 -> 391	LVWVGRVMVLVVALVAIALAA
	275 -> 295	MTWMILCLAGAVAVGFFGIAY
	341 -> 370	TLSCQLLVCSSAITEDLYKAFLRKHASQKE
	376 -> 376	R
	446 -> 448	QFG
	232 -> 248	GLNFVAIISLMGWGLGY
	257 -> 257	R
	424 -> 430	RMTRNGA
	27 -> 66	RSTKNFDDYILGGRSLGPFVTALSAGASDMSGWLLMGLPG
	88 -> 124	INWKLVAGRLRVHTEYNNNALTLPDYFTGRFEDKSRI
	190 -> 213	QASLMIFALILTPVIVIISVGGFG
	449 -> 475	WLGLYEIIPGFIFGSIGIVVFSLLGKA
	185 -> 189	WTDTV
	249 -> 274	FGQPHILARFMAADSHHSIVHARRIS
	398 -> 423	LGLVSYAWAGFGAAFGPVVLFSVMWS
	431 -> 445	LAGMIIGALTVIVWK
	4 -> 26	STPMLVTFCVYIFGMILIGFIAW
	151 -> 162	LFESTFGMSYET
	214 -> 231	DSLEVIKQKSIENVDMLK
	296 -> 319	FNDHPALAGAVNQNAERVFIELAQ
	392 -> 397	NPENRV

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0015293 - symporter activity GO:0022857 - transmembrane transporter activity GO:0005298 - proline:sodium symporter activity GO:0031402 - sodium ion binding
	biological_process	GO:0006811 - ion transport GO:0006865 - amino acid transport GO:0015824 - proline transport GO:0006814 - sodium ion transport GO:0015912 - short-chain fatty acid transport GO:0055085 - transmembrane transport GO:0035524 - proline transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Pirch T., et al., 2003
[2] Quick M., et al., 1997
[3] Quick M., et al., 1998
[4] Quick M., et al., 1999

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

PRODB:

PROSITE:

PROSITE:

PROSITE:

REFSEQ:

UNIPROT:

Operon

Name:

putP

Operon arrangement:

Transcription unit	Promoter
putP	putPp1
putP	putPp2
putP	putPp3
putP	putPp4
putP	putPp5

Transcriptional Regulation
	Display Regulation
Repressed by:	CRP, PutA

RNA cis-regulatory element

Attenuation:	Translational

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_1317	1079288	forward	nd	[RS-EPT-CBR]	[5]
promoter	efeOp2	1082154	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 10.215. P-value: 2.353e-6 Read more >	[ICWHO]	[6]
promoter	efeOp1	1082243	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.763. P-value: 6.773e-5 Read more >	[ICWHO]	[6]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Pirch T., Landmeier S., Jung H., 2003, Transmembrane domain II of the Na+/proline transporter PutP of Escherichia coli forms part of a conformationally flexible, cytoplasmic exposed aqueous cavity within the membrane., J Biol Chem 278(44):42942-9
[2] Quick M., Jung H., 1997, Aspartate 55 in the Na+/proline permease of Escherichia coli is essential for Na+-coupled proline uptake., Biochemistry 36(15):4631-6
[3] Quick M., Jung H., 1998, A conserved aspartate residue, Asp187, is important for Na+-dependent proline binding and transport by the Na+/proline transporter of Escherichia coli., Biochemistry 37(39):13800-6
[4] Quick M., Stolting S., Jung H., 1999, Role of conserved Arg40 and Arg117 in the Na+/proline transporter of Escherichia coli., Biochemistry 38(41):13523-9
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78