RegulonDB RegulonDB 10.10: Gene Form
   

rbsK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rbsR rbsK rbsB 3\' rbsB hsrA terminator anti-terminator TSS_4366 (cluster) TSS_4366 (cluster)

Gene      
Name: rbsK    Texpresso search in the literature
Synonym(s): ECK3746, EG10818, b3752
Genome position(nucleotides): 3937294 --> 3938223 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.94
External database links:  
ASAP:
 ABE-0012266
CGSC:
 315
ECHOBASE:
 EB0811
ECOLIHUB:
 rbsK
OU-MICROARRAY:
 b3752
STRING:
 511145.b3752
COLOMBOS: rbsK


Product      
Name: ribokinase
Synonym(s): RbsK
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 32.29
Isoelectric point: 4.736
Motif(s):
 
Type Positions Sequence
223 -> 228 TLGSRG
6 -> 297 SLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKGANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFVNGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNKTIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIRTVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIRFAHAAAAIAVTRKGAQPSVPW
42 -> 46 GKGAN
254 -> 255 GD
14 -> 16 NAD

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0016773 - phosphotransferase activity, alcohol group as acceptor
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0004747 - ribokinase activity
GO:0042803 - protein homodimerization activity
biological_process GO:0005975 - carbohydrate metabolic process
GO:0016310 - phosphorylation
GO:0006014 - D-ribose metabolic process
GO:0019303 - D-ribose catabolic process
GO:0046835 - carbohydrate phosphorylation
Note(s): Note(s): ...[more].
Reference(s): [1] David J., et al., 1970
[2] David J., et al., 1970
[3] Desroy N., et al., 2009
[4] Mowbray SL., et al., 1999
[5] Parkin DW., et al., 1984
[6] Roche ED., et al., 2001
External database links:  
DIP:
 DIP-36178N
ECOCYC:
 RIBOKIN-MONOMER
ECOLIWIKI:
 b3752
INTERPRO:
 IPR029056
INTERPRO:
 IPR011877
INTERPRO:
 IPR002139
INTERPRO:
 IPR002173
INTERPRO:
 IPR011611
MODBASE:
 P0A9J6
PDB:
 1RKS
PDB:
 1RKD
PDB:
 1RKA
PDB:
 1RK2
PDB:
 1GQT
PFAM:
 PF00294
PRIDE:
 P0A9J6
PRINTS:
 PR00990
PROSITE:
 PS00584
REFSEQ:
 NP_418208
SMR:
 P0A9J6
UNIPROT:
 P0A9J6


Operon      
Name: rbsDACBKR         
Operon arrangement:
Transcription unit        Promoter
rbsDACBKR


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: RbsR


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4366 (cluster) 3937237 forward nd [RS-EPT-CBR] [7]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] David J., Wiesmeyer H., 1970, Regulation of ribose metabolism in Escherichia coli. I. The ribose catabolic pathway., Biochim Biophys Acta 208(1):45-55

 [2] David J., Wiesmeyer H., 1970, Regulation of ribose metabolism in Escherichia coli. 3. Regulation of ribose utilization in vivo., Biochim Biophys Acta 208(1):68-76

 [3] Desroy N., Moreau F., Briet S., Le Fralliec G., Floquet S., Durant L., Vongsouthi V., Gerusz V., Denis A., Escaich S., 2009, Towards Gram-negative antivirulence drugs: new inhibitors of HldE kinase., Bioorg Med Chem 17(3):1276-89

 [4] Mowbray SL., Helgstrand C., Sigrell JA., Cameron AD., Jones TA., 1999, Errors and reproducibility in electron-density map interpretation., Acta Crystallogr D Biol Crystallogr 55(Pt 7):1309-19

 [5] Parkin DW., Leung HB., Schramm VL., 1984, Synthesis of nucleotides with specific radiolabels in ribose. Primary 14C and secondary 3H kinetic isotope effects on acid-catalyzed glycosidic bond hydrolysis of AMP, dAMP, and inosine., J Biol Chem 259(15):9411-7

 [6] Roche ED., Sauer RT., 2001, Identification of endogenous SsrA-tagged proteins reveals tagging at positions corresponding to stop codons., J Biol Chem 276(30):28509-15

 [7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

RegulonDB