RegulonDB

Gene
Name:	rcsA
Synonym(s):	ECK1949, EG10820, b1951, cpsR
Genome position(nucleotides):	2023968 --> 2024591 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	38.14
External database links:

ASAP:	ABE-0006482
CGSC:	17980
ECHOBASE:	EB0813
OU-MICROARRAY:	b1951
PortEco:	rcsA
STRING:	511145.b1951
COLOMBOS:	rcsA

Product

Name:

DNA-binding transcriptional activator RcsA

Synonym(s):

CpsR, RcsA, positive DNA-binding transcriptional regulator of capsular polysaccharide synthesis, activates its own expression, positive regulator of capsular polysaccharide synthesis

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

23.516

Isoelectric point:

9.692

Motif(s):

Type	Positions	Sequence
	137 -> 191	SLSRTESSMLRMWMAGQGTIQISDQMNIKAKTVSSHKGNIKRKIKTHNKQVIYHV
	145 -> 145	M
	131 -> 196	LNMPTLSLSRTESSMLRMWMAGQGTIQISDQMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTD
	155 -> 174	TIQISDQMNIKAKTVSSHKG

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.2 - colanic acid (M antigen)
		1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.5 - K antigen
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
		6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding
	biological_process	GO:0006351 - transcription, DNA-templated GO:0006355 - regulation of transcription, DNA-templated GO:0050896 - response to stimulus

Note(s):

Note(s): ...[more].

Reference(s):

[1] Da Re S., et al., 2013
[2] Gottesman S., et al., 1991
[3] Hersch SJ., et al., 2020
[4] Sledjeski DD., et al., 1996
[5] Stout V., et al., 1990

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	RcsAB, GadE
Repressed by:	H-NS

Reference(s)
[1] Da Re S., Valle J., Charbonnel N., Beloin C., Latour-Lambert P., Faure P., Turlin E., Le Bouguenec C., Renauld-Mongenie G., Forestier C., Ghigo JM., 2013, Identification of commensal Escherichia coli genes involved in biofilm resistance to pathogen colonization., PLoS One 8(5):e61628
[2] Gottesman S., Stout V., 1991, Regulation of capsular polysaccharide synthesis in Escherichia coli K12., Mol Microbiol 5(7):1599-606
[3] Hersch SJ., Watanabe N., Stietz MS., Manera K., Kamal F., Burkinshaw B., Lam L., Pun A., Li M., Savchenko A., Dong TG., 2020, Envelope stress responses defend against type six secretion system attacks independently of immunity proteins., Nat Microbiol
[4] Sledjeski DD., Gottesman S., 1996, Osmotic shock induction of capsule synthesis in Escherichia coli K-12., J Bacteriol 178(4):1204-6
[5] Stout V., Gottesman S., 1990, RcsB and RcsC: a two-component regulator of capsule synthesis in Escherichia coli., J Bacteriol 172(2):659-69