RegulonDB RegulonDB 10.6.3: Gene Form
   

recN gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

recN nadK bamE NsrR CpxR DnaA LexA DnaA LexA CRP TSS_2970 TSS_2970 TSS_2969 TSS_2969 TSS_2968 TSS_2968 TSS_2967 TSS_2967 TSS_2966 TSS_2966 TSS_2965 TSS_2965 bamEp bamEp TSS_2964 TSS_2964 recNp recNp

Gene      
Name: recN    Texpresso search in the literature
Synonym(s): ECK2612, EG10831, b2616, radB
Genome position(nucleotides): 2751795 --> 2753456 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.81
External database links:  
ASAP:
ABE-0008611
CGSC:
10872
ECHOBASE:
EB0824
ECOCYC:
EG10831
ECOLIHUB:
recN
OU-MICROARRAY:
b2616
REGULONDB:
b2616
STRING:
511145.b2616
M3D: recN
COLOMBOS: recN
PortEco: b2616


Product      
Name: DNA repair protein RecN
Synonym(s): RadB, RecN
Sequence: Get amino acid sequence Fasta Format
Cellular location: bacterial nucleoid,cytosol
Molecular weight: 61.396
Isoelectric point: 5.347
Motif(s):
 
Type Positions Sequence
35 -> 35 K
535 -> 553 GGSEVTRNTLANAKELLAA
59 -> 60 AA
76 -> 76 A
1 -> 511 MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGLCLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGRSRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMTARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLANSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQIAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQQLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQFTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARKMETPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKETDG

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.1 - DNA related --> 2.1.3 - DNA recombination
  2 - information transfer --> 2.1 - DNA related --> 2.1.4 - DNA repair
  5 - cell processes --> 5.8 - SOS response
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0043590 - bacterial nucleoid
molecular_function GO:0005515 - protein binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
biological_process GO:0010165 - response to X-ray
GO:0036298 - recombinational interstrand cross-link repair
GO:0006281 - DNA repair
GO:0006310 - DNA recombination
GO:0006974 - cellular response to DNA damage stimulus
GO:0009432 - SOS response
GO:0009314 - response to radiation
GO:0009411 - response to UV
GO:0000725 - recombinational repair
GO:0006302 - double-strand break repair
GO:0010212 - response to ionizing radiation
GO:0000724 - double-strand break repair via homologous recombination
Note(s): Note(s): ...[more].
Evidence: [IMP] Inferred from mutant phenotype
Reference(s): [1] Almeida E., et al., 2010
[2] Brena-Valle M., et al., 1998
[3] Courcelle J., et al., 2001
[4] Cupido M., et al., 1985
[5] Dunman PM., et al., 2000
[6] Keyamura K., et al., 2013
[7] Lloyd RG., et al., 1991
[8] Lloyd RG., et al., 1983
[9] Odsbu I., et al., 2014
[10] Picksley SM., et al., 1984
[11] Rostas K., et al., 1987
[12] Sargentini NJ., et al., 1988
[13] Sargentini NJ., et al., 1986
[14] Sargentini NJ., et al., 1983
[15] Takahashi NK., et al., 1993
[16] Vickridge E., et al., 2017
[17] Warr AR., et al., 2018
External database links:  
DIP:
DIP-10655N
ECOCYC:
EG10831-MONOMER
ECOLIWIKI:
b2616
INTERPRO:
IPR003395
INTERPRO:
IPR027417
INTERPRO:
IPR004604
MINT:
MINT-1269449
MODBASE:
P05824
PANTHER:
PTHR11059
PFAM:
PF02463
PRIDE:
P05824
PRODB:
PRO_000023704
PROTEINMODELPORTAL:
P05824
REFSEQ:
YP_026172
SMR:
P05824
UNIPROT:
P05824


Operon      
Name: recN         
Operon arrangement:
Transcription unit        Promoter
recN


Transcriptional Regulation      
Display Regulation             
Repressed by: DnaA, LexA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2964 2752658 reverse nd [RS-EPT-CBR] [18]
  promoter TSS_2965 2753488 forward nd [RS-EPT-CBR] [18]
  promoter TSS_2966 2753555 forward nd [RS-EPT-CBR] [18]
  promoter TSS_2967 2753588 forward nd [RS-EPT-CBR] [18]
  promoter TSS_2968 2753594 forward nd [RS-EPT-CBR] [18]
  promoter TSS_2969 2753599 forward nd [RS-EPT-CBR] [18]
  promoter TSS_2970 2753605 forward nd [RS-EPT-CBR] [18]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Almeida E., Fuentes JL., Cuetara E., Prieto E., Llagostera M., 2010, Amifostine protection against induced DNA damage in gamma-irradiated Escherichia coli cells depend on recN DNA repair gene product activity., Environ Toxicol 25(2):130-6

 [2] Brena-Valle M., Serment-Guerrero J., 1998, SOS induction by gamma-radiation in Escherichia coli strains defective in repair and/or recombination mechanisms., Mutagenesis 13(6):637-41

 [3] Courcelle J., Khodursky A., Peter B., Brown PO., Hanawalt PC., 2001, Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient Escherichia coli., Genetics 158(1):41-64

 [4] Cupido M., Bridges BA., 1985, Uvr-independent repair of 8-methoxypsoralen crosslinks in Escherichia coli: evidence for a recombinational process., Mutat Res 146(2):135-41

 [5] Dunman PM., Ren L., Rahman MS., Palejwala VA., Murphy HS., Volkert MR., Humayun MZ., 2000, Escherichia coli cells defective for the recN gene display constitutive elevation of mutagenesis at 3,N(4)-ethenocytosine via an SOS-induced mechanism., Mol Microbiol 37(3):680-6

 [6] Keyamura K., Sakaguchi C., Kubota Y., Niki H., Hishida T., 2013, RecA protein recruits structural maintenance of chromosomes (SMC)-like RecN protein to DNA double-strand breaks., J Biol Chem 288(41):29229-37

 [7] Lloyd RG., Buckman C., 1991, Overlapping functions of recD, recJ and recN provide evidence of three epistatic groups of genes in Escherichia coli recombination and DNA repair., Biochimie 73(2-3):313-20

 [8] Lloyd RG., Picksley SM., Prescott C., 1983, Inducible expression of a gene specific to the RecF pathway for recombination in Escherichia coli K12., Mol Gen Genet 190(1):162-7

 [9] Odsbu I., Skarstad K., 2014, DNA compaction in the early part of the SOS response is dependent on RecN and RecA., Microbiology 160(Pt 5):872-82

 [10] Picksley SM., Attfield PV., Lloyd RG., 1984, Repair of DNA double-strand breaks in Escherichia coli K12 requires a functional recN product., Mol Gen Genet 195(1-2):267-74

 [11] Rostas K., Morton SJ., Picksley SM., Lloyd RG., 1987, Nucleotide sequence and LexA regulation of the Escherichia coli recN gene., Nucleic Acids Res 15(13):5041-9

 [12] Sargentini NJ., Smith KC., 1988, Genetic and phenotypic analyses indicating occurrence of the recN262 and radB101 mutations at the same locus in Escherichia coli., J Bacteriol 170(5):2392-4

 [13] Sargentini NJ., Smith KC., 1986, Quantitation of the involvement of the recA, recB, recC, recF, recJ, recN, lexA, radA, radB, uvrD, and umuC genes in the repair of X-ray-induced DNA double-strand breaks in Escherichia coli., Radiat Res 107(1):58-72

 [14] Sargentini NJ., Smith KC., 1983, Characterization of an Escherichia coli mutant (radB101) sensitive to gamma and uv radiation, and methyl methanesulfonate., Radiat Res 93(3):461-78

 [15] Takahashi NK., Kusano K., Yokochi T., Kitamura Y., Yoshikura H., Kobayashi I., 1993, Genetic analysis of double-strand break repair in Escherichia coli., J Bacteriol 175(16):5176-85

 [16] Vickridge E., Planchenault C., Cockram C., Junceda IG., Espeli O., 2017, Management of E. coli sister chromatid cohesion in response to genotoxic stress., Nat Commun 8:14618

 [17] Warr AR., Klimova AN., Nwaobasi AN., Sandler SJ., 2018, Protease-deficient SOS constitutive cells have RecN-dependent cell division phenotypes., Mol Microbiol

 [18] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu¿¿iz-Rascado L, Garc¿¿a-Sotelo JS, Weiss V, Solano-Lira H, Mart¿¿nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern¿¿ndez S, Alquicira-Hern¿¿ndez K, L¿¿pez-Fuentes A, Porr¿¿n-Sotelo L, Huerta AM, Bonavides-Mart¿¿nez C, Balderas-Mart¿¿nez YI, Pannier L, Olvera M, Labastida A, Jim¿¿nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch¿¿vez V, Hern¿¿ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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