RegulonDB RegulonDB 10.9: Gene Form
   

rfe gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rho rfe wzzE NsrR terminator TSS_4443 TSS_4443 TSS_4442 TSS_4442 TSS_4441 (cluster) TSS_4441 (cluster) TSS_4440 TSS_4440 TSS_4439 TSS_4439 TSS_4438 TSS_4438 TSS_4437 (cluster) TSS_4437 (cluster) TSS_4436 TSS_4436 rfep rfep TSS_4435 TSS_4435 TSS_4434 TSS_4434 TSS_4433 TSS_4433

Gene      
Name: rfe    Texpresso search in the literature
Synonym(s): ECK3776, EG10840, b3784, wecA
Genome position(nucleotides): 3967916 --> 3969019 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
49.46
External database links:  
ASAP:
ABE-0012366
CGSC:
294
ECHOBASE:
EB0833
ECOLIHUB:
rfe
OU-MICROARRAY:
b3784
STRING:
511145.b3784
COLOMBOS: rfe


Product      
Name: UDP-N-acetylglucosamine—undecaprenyl-phosphate N-acetylglucosaminephosphotransferase
Synonym(s): Rfe, WecA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,cell wall
Molecular weight: 40.957
Isoelectric point: 10.467
Motif(s):
 
Type Positions Sequence
75 -> 232 LYLACAGVLVFIGALDDRFDISVKIRATIQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDGLLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDAGSTLIGFTVIWILL
159 -> 159 D
158 -> 178 IDGLLGGLSCVSFAAIGMILW
242 -> 262 ISPVTALWIIAIPLMDMVAIM
294 -> 314 AFVLITLAAALLASIGVLAEY

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.4 - enterobacterial common antigen (surface glycolipid)
  6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0009276 - Gram-negative-bacterium-type cell wall
molecular_function GO:0016757 - transferase activity, transferring glycosyl groups
GO:0016740 - transferase activity
GO:0016780 - phosphotransferase activity, for other substituted phosphate groups
GO:0008963 - phospho-N-acetylmuramoyl-pentapeptide-transferase activity
GO:0000287 - magnesium ion binding
GO:0008144 - drug binding
GO:0030145 - manganese ion binding
GO:0042802 - identical protein binding
GO:0036380 - UDP-N-acetylglucosamine-undecaprenyl-phosphate N-acetylglucosaminephosphotransferase activity
biological_process GO:0009103 - lipopolysaccharide biosynthetic process
GO:0009243 - O antigen biosynthetic process
GO:0009246 - enterobacterial common antigen biosynthetic process
GO:0044038 - cell wall macromolecule biosynthetic process
GO:0071555 - cell wall organization
External database links:  
DIP:
DIP-48083N
ECOCYC:
GLCNACPTRANS-MONOMER
ECOLIWIKI:
b3784
INTERPRO:
IPR012750
INTERPRO:
IPR000715
PANTHER:
PTHR22926
PFAM:
PF00953
PRIDE:
P0AC78
PRODB:
PRO_000024218
REFSEQ:
NP_418231
UNIPROT:
P0AC78


Operon      
Name: rfe-wzzE-wecBC-rffGHC-wecE-wzxE-wecF-wzyE-rffM         
Operon arrangement:
Transcription unit        Promoter
rfe-wzzE-wecBC-rffGHC-wecE-wzxE-wecF-wzyE-rffM


Transcriptional Regulation      
Display Regulation             
Repressed by: NsrR


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4433 3966439 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4434 3967728 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4435 3967746 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4436 3967890 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4437 (cluster) 3967895 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4438 3968862 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4439 3969127 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4440 3969130 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4441 (cluster) 3969176 forward For this promoter, there
Read more >
[RS-EPT-CBR] [1]
  promoter TSS_4442 3969944 forward nd [RS-EPT-CBR] [1]
  promoter TSS_4443 3969950 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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