RegulonDB RegulonDB 10.7: Gene Form
   

rplS gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

trmD yfiB rplS rpsP5\' TSS_2951 TSS_2951 TSS_2950 TSS_2950 TSS_2949 TSS_2949 TSS_2948 TSS_2948 rplSp1 rplSp1 TSS_2947 TSS_2947

Gene      
Name: rplS    Texpresso search in the literature
Synonym(s): ECK2603, EG10880, b2606
Genome position(nucleotides): 2744183 <-- 2744530 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
48.28
External database links:  
ASAP:
ABE-0008574
CGSC:
247
ECHOBASE:
EB0873
OU-MICROARRAY:
b2606
PORTECO:
rplS
REGULONDB:
b2606
STRING:
511145.b2606
M3D: rplS
COLOMBOS: rplS
PortEco: b2606


Product      
Name: 50S ribosomal subunit protein L19
Synonym(s): RplS
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,ribosome
Molecular weight: 13.133
Isoelectric point: 11.301
Motif(s):
 
Type Positions Sequence
3 -> 113 NIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRERTGKAARIKER
2 -> 115 SNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFEGVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRERTGKAARIKERLN

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.2 - translation
  2 - information transfer --> 2.3 - protein related --> 2.3.8 - ribosomal proteins
  6 - cell structure --> 6.6 - ribosomes
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0005840 - ribosome
GO:0022625 - cytosolic large ribosomal subunit
molecular_function GO:0003735 - structural constituent of ribosome
GO:0003723 - RNA binding
GO:0019843 - rRNA binding
GO:0070180 - large ribosomal subunit rRNA binding
biological_process GO:0006412 - translation
GO:0000027 - ribosomal large subunit assembly
Note(s): Note(s): ...[more].
Reference(s): [1] Adachi K., et al., 1979
[2] Bernabeu C., et al., 1979
[3] Brosius J., et al., 1978
[4] Bystrom AS., et al., 1983
[5] Dabbs ER., et al., 1983
[6] Dzionara M., et al., 1977
[7] Gimautdinova OI., et al., 1981
[8] Highland JH., et al., 1974
[9] Isono K. 1978
[10] Isono K., et al., 1976
[11] Kitakawa M., et al., 1980
[12] Morrison CA., et al., 1977
[13] Saito H., et al., 1978
[14] Siegrist S., et al., 1985
[15] Stoffler-Meilicke M., et al., 1983
[16] Tischendorf GW., et al., 1974
[17] Tumanova LG., et al., 1984
[18] Tumanova LG., et al., 1983
External database links:  
DIP:
DIP-35793N
ECOCYC:
EG10880-MONOMER
ECOLIWIKI:
b2606
INTERPRO:
IPR001857
INTERPRO:
IPR008991
INTERPRO:
IPR038657
INTERPRO:
IPR018257
MINT:
MINT-1245288
PANTHER:
PTHR15680
PDB:
2J28
PDB:
2RDO
PDB:
3BBX
PDB:
3J5L
PDB:
3J7Z
PDB:
3J8G
PDB:
3J9Y
PDB:
3J9Z
PDB:
3JA1
PDB:
3JBU
PDB:
3JBV
PDB:
3JCD
PDB:
3JCE
PDB:
3JCJ
PDB:
3JCN
PDB:
4CSU
PDB:
4U1U
PDB:
4U1V
PDB:
4U20
PDB:
4U24
PDB:
4U25
PDB:
4U26
PDB:
4U27
PDB:
4UY8
PDB:
4V47
PDB:
4V48
PDB:
4V4H
PDB:
4V4Q
PDB:
4V4V
PDB:
4V4W
PDB:
4V50
PDB:
4V52
PDB:
4V53
PDB:
4V54
PDB:
4V55
PDB:
4V56
PDB:
4V57
PDB:
4V5B
PDB:
4V5H
PDB:
4V5Y
PDB:
4V64
PDB:
4V65
PDB:
4V66
PDB:
4V69
PDB:
4V6C
PDB:
4V6D
PDB:
4V6E
PDB:
4V6K
PDB:
4V6L
PDB:
4V6M
PDB:
4V6N
PDB:
4V6O
PDB:
4V6P
PDB:
4V6Q
PDB:
4V6R
PDB:
4V6S
PDB:
4V6T
PDB:
4V6V
PDB:
4V6Y
PDB:
4V6Z
PDB:
4V70
PDB:
4V71
PDB:
4V72
PDB:
4V73
PDB:
4V74
PDB:
4V75
PDB:
4V76
PDB:
4V77
PDB:
4V78
PDB:
4V79
PDB:
4V7A
PDB:
4V7B
PDB:
4V7C
PDB:
4V7D
PDB:
4V7I
PDB:
4V7S
PDB:
4V7T
PDB:
4V7U
PDB:
4V7V
PDB:
4V85
PDB:
4V89
PDB:
4V9C
PDB:
4V9D
PDB:
4V9O
PDB:
4V9P
PDB:
4WF1
PDB:
4WOI
PDB:
4WWW
PDB:
4YBB
PDB:
5ADY
PDB:
5AFI
PDB:
5AKA
PDB:
5GAD
PDB:
5GAE
PDB:
5GAF
PDB:
5GAG
PDB:
5GAH
PDB:
5H5U
PDB:
5IQR
PDB:
5IT8
PDB:
5J5B
PDB:
5J7L
PDB:
5J88
PDB:
5J8A
PDB:
5J91
PDB:
5JC9
PDB:
5JTE
PDB:
5JU8
PDB:
5KCR
PDB:
5KCS
PDB:
5KPS
PDB:
5KPV
PDB:
5KPW
PDB:
5KPX
PDB:
5L3P
PDB:
5LZA
PDB:
5LZB
PDB:
5LZC
PDB:
5LZD
PDB:
5LZE
PDB:
5LZF
PDB:
5MDV
PDB:
5MDW
PDB:
5MDY
PDB:
5MDZ
PDB:
5MGP
PDB:
5NCO
PDB:
5NP6
PDB:
5NWY
PDB:
5O2R
PDB:
5U4I
PDB:
5U9F
PDB:
5U9G
PDB:
5UYK
PDB:
5UYL
PDB:
5UYM
PDB:
5UYN
PDB:
5UYP
PDB:
5UYQ
PDB:
5WDT
PDB:
5WE4
PDB:
5WE6
PDB:
5WFK
PDB:
6BU8
PDB:
6BY1
PDB:
6C4I
PDB:
6ENF
PDB:
6ENJ
PDB:
6ENU
PDB:
6GBZ
PDB:
6GC0
PDB:
6GC4
PDB:
6GC6
PDB:
6GC7
PDB:
6GC8
PDB:
6GWT
PDB:
6GXM
PDB:
6GXN
PDB:
6GXO
PDB:
6GXP
PDB:
6H4N
PDB:
6H58
PDB:
6HRM
PDB:
6I0Y
PDB:
6I7V
PDB:
6Q97
PDB:
6Q98
PDB:
6Q9A
PDB:
6QUL
PFAM:
PF01245
PRIDE:
P0A7K6
PRINTS:
PR00061
PRODB:
PRO_000023825
PROSITE:
PS01015
PROTEINMODELPORTAL:
P0A7K6
REFSEQ:
NP_417097
SMR:
P0A7K6
UNIPROT:
P0A7K6


Operon      
Name: rpsP-rimM-trmD-rplS         
Operon arrangement:
Transcription unit        Promoter
rpsP-rimM-trmD-rplS


Transcriptional Regulation      
Display Regulation             
Repressed by: FNR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2947 2744532 reverse nd [RS-EPT-CBR] [19]
  promoter rplSp1 2744642 reverse Similarity to the consensus
Read more >
[ICWHO] [20]
  promoter TSS_2948 2744644 reverse nd [RS-EPT-CBR] [19]
  promoter TSS_2949 2744647 reverse nd [RS-EPT-CBR] [19]
  promoter TSS_2950 2744657 reverse nd [RS-EPT-CBR] [19]
  promoter TSS_2951 2744665 reverse nd [RS-EPT-CBR] [19]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Adachi K., Sells BH., 1979, The effect of magnesium starvation on the dissociation of ribosomal proteins from Escherichia coli K-12 ribosomes., Biochim Biophys Acta 563(1):163-70

 [2] Bernabeu C., Vazquez D., Conde FP., 1979, Comparative study between prokaryotes and eukaryotes by chemical iodination of ribosomal proteins., Biochim Biophys Acta 577(2):400-9

 [3] Brosius J., Arfsten U., 1978, Primary structure of protein L19 from the large subunit of Escherichia coli ribosomes., Biochemistry 17(3):508-16

 [4] Bystrom AS., Hjalmarsson KJ., Wikstrom PM., Bjork GR., 1983, The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide., EMBO J 2(6):899-905

 [5] Dabbs ER., Hasenbank R., Kastner B., Rak KH., Wartusch B., Stoffler G., 1983, Immunological studies of Escherichia coli mutants lacking one or two ribosomal proteins., Mol Gen Genet 192(3):301-8

 [6] Dzionara M., Robinson SM., Wittmann-Liebold B., 1977, Prediction for secondary structures of ten proteins from the 50S subunit of the Escherichia coli ribosome., J Supramol Struct 7(2):191-204

 [7] Gimautdinova OI., Karpova GG., Knorre DG., Kobetz ND., 1981, The proteins of the messenger RNA binding site of Escherichia coli ribosomes., Nucleic Acids Res 9(14):3465-81

 [8] Highland JH., Ochsner E., Gordon J., Bodley JW., Hasenbank R., Stoffler G., 1974, Coordinate inhibition of elongation factor G function and ribosomal subunit association by antibodies to several ribosomal proteins., Proc Natl Acad Sci U S A 71(3):627-30

 [9] Isono K., 1978, Genes encoding ribosomal proteins S16 and L19 form a gene cluster at 56.4 min in Escherichia coli., Mol Gen Genet 165(3):265-8

 [10] Isono K., Krauss J., Hirota Y., 1976, Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins., Mol Gen Genet 149(3):297-302

 [11] Kitakawa M., Blumenthal L., Isono K., 1980, Isolation and characterization of specialized transducing lambda phages carrying ribosomal protein genes of Escherichia coli., Mol Gen Genet 180(2):343-9

 [12] Morrison CA., Tischendorf G., Stoffler G., Garrett RA., 1977, Accessibility of proteins in 50S ribosomal subunits of Escherichia coli to antibodies: an ultracentrifugation study., Mol Gen Genet 151(3):245-52

 [13] Saito H., Nakamura Y., Uchida H., 1978, A transducing lambda phage carrying grpE, a bacterial gene necessary for lambda DNA replication, and two ribosomal protein genes, rpsP (S16) and rplS (L19)., Mol Gen Genet 165(3):247-56

 [14] Siegrist S., Moreau N., Le Goffic F., 1985, About the specificity of photoinduced affinity labeling of Escherichia coli ribosomes by dihydrorosaramicin, a macrolide related to erythromycin., Eur J Biochem 153(1):131-5

 [15] Stoffler-Meilicke M., Noah M., Stoffler G., 1983, Location of eight ribosomal proteins on the surface of the 50S subunit from Escherichia coli., Proc Natl Acad Sci U S A 80(22):6780-4

 [16] Tischendorf GW., Zeichhardt H., Stoffler G., 1974, Determination of the location of proteins L14, L17, L18, L19, L22, L23 on the surface of the 5oS ribosomal subunit of Escherichia coli by immune electron microscopy., Mol Gen Genet 134(3):187-208

 [17] Tumanova LG., Gongadze GM., Ven'iaminov SIu., Gudkov AT., Bushuev VN., 1984, [Comparison of the physical properties of ribosomal proteins from Escherichia coli 50S subparticles isolated by different methods]., Mol Biol (Mosk) 18(3):751-8

 [18] Tumanova LG., Gongadze GM., Venyaminov SYu., Gudkov AT., Bushuev VN., 1983, Physical properties of ribosomal proteins isolated under different conditions from the Escherichia coli 50 S subunit., FEBS Lett 157(1):85-90

 [19] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [20] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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