RegulonDB

Gene
Name:	rpsT
Synonym(s):	ECK0024, EG10919, b0023, sup(s20), supS, supS20
Genome position(nucleotides):	20815 <-- 21078 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	46.59
External database links:

ASAP:	ABE-0000082
CGSC:	212
ECHOBASE:	EB0912
OU-MICROARRAY:	b0023
PORTECO:	rpsT
REGULONDB:	b0023
STRING:	511145.b0023
M3D:	rpsT
COLOMBOS:	rpsT
PortEco:	b0023

Product

Name:

30S ribosomal subunit protein S20

Synonym(s):

RpsT, SupS

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol,ribosome

Molecular weight:

9.684

Isoelectric point:

11.828

Motif(s):

Type	Positions	Sequence
	38 -> 38	A
	2 -> 87	ANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDKAAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.3 - protein related --> 2.3.2 - translation
		2 - information transfer --> 2.3 - protein related --> 2.3.8 - ribosomal proteins
		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.3 - inhibition / activation of enzymes
		6 - cell structure --> 6.6 - ribosomes
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:0005840 - ribosome GO:0022627 - cytosolic small ribosomal subunit
	molecular_function	GO:0003735 - structural constituent of ribosome GO:0008073 - ornithine decarboxylase inhibitor activity GO:0003723 - RNA binding GO:0019843 - rRNA binding GO:0070181 - small ribosomal subunit rRNA binding
	biological_process	GO:0006412 - translation GO:0043086 - negative regulation of catalytic activity GO:0000028 - ribosomal small subunit assembly

Note(s):

Note(s): ...[more].

Reference(s):

[1] Daya-Grosjean L., et al., 1974
[2] Ungewickell E., et al., 1975

External database links:

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REFSEQ:

SMR:

UNIPROT:

Operon

Name:

rpsT

Operon arrangement:

Transcription unit	Promoter
rpsT	rpsTp2
rpsT	rpsTp1

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_44 (cluster)	20342		forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 20342 to 20343, where the highest frequency is at position 20343. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]
promoter	TSS_45 (cluster)	20678		reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 20677 to 20678, where the highest frequency is at position 20677. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]
promoter	TSS_46	20910		reverse	nd	[RS-EPT-CBR]	[3]
transcriptional_attenuator	anti-anti-terminator	20912	20935	forward	nd	[ICA]	[4]
transcriptional_attenuator	anti-terminator	20912	20955	forward	nd	[ICA]	[4]
transcriptional_attenuator	terminator	20937	20982	forward	nd	[ICA]	[4]
promoter	TSS_47	21020		reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_48	21045		reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_49	21094		reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_50	21098		reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_51	21112		reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_53	21155		reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_54	21157		reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_55	21159		reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_56 (cluster)	21208		reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 21207 to 21208, where the highest frequency is at position 21207. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICA] Inferred by computational analysis

Reference(s)
[1] Daya-Grosjean L., Reinbolt J., Pongs O., Garrett RA., 1974, A study of the regions of ribosomal proteins S4, S8, S15 and S20 that interact with 16 S RNA of Escherichia coli., FEBS Lett 44(3):253-6
[2] Ungewickell E., Garrett R., Ehresmann C., Stiegler P., Fellner P., 1975, An investigation of the 16-S RNA binding sites of ribosomal proteins S4, S8, S15, and S20 FROM Escherichia coli., Eur J Biochem 51(1):165-80
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[4] Merino E, Yanofsky C., 2005, Transcription attenuation: a highly conserved regulatory strategy used by bacteria., Trends Genet. 2005 May;21(5):260-4.