RegulonDB RegulonDB 10.8: Gene Form
   

secA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

secA secM mutT terminator anti-terminator anti-anti-terminator terminator TSS_221 TSS_221 TSS_220 (cluster) TSS_220 (cluster) TSS_219 TSS_219 TSS_218 TSS_218 TSS_217 TSS_217 TSS_216 (cluster) TSS_216 (cluster) TSS_215 (cluster) TSS_215 (cluster) TSS_214 TSS_214 TSS_213 TSS_213 TSS_212 TSS_212 TSS_211 TSS_211 TSS_210 TSS_210 TSS_209 (cluster) TSS_209 (cluster) TSS_208 TSS_208 TSS_207 TSS_207 TSS_206 TSS_206 TSS_205 TSS_205 TSS_204 TSS_204 TSS_203 TSS_203 TSS_202 TSS_202 TSS_201 TSS_201 TSS_200 TSS_200 TSS_199 TSS_199 TSS_198 TSS_198 TSS_197 TSS_197

Gene      
Name: secA    Texpresso search in the literature
Synonym(s): ECK0099, EG10936, azi, b0098, pea, prlD
Genome position(nucleotides): 108279 --> 110984 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.4
Reference(s): [1] Schmidt MG., et al., 1988
External database links:  
ASAP:
 ABE-0000343
CGSC:
 176
ECHOBASE:
 EB0929
OU-MICROARRAY:
 b0098
PortEco:
 secA
STRING:
 511145.b0098
COLOMBOS: secA


Product      
Name: protein translocation ATPase
Synonym(s): Azi, Pea, PrlD, SecA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,inner membrane
Molecular weight: 102.023
Isoelectric point: 5.308
Motif(s):
 
Type Positions Sequence
7 -> 402 TKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFS
134 -> 134 Y
198 -> 210 RVQRKLHYALVDE
102 -> 109 MRTGEGKT
488 -> 488 A

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.5 - The Type II (General) Secretory Pathway (IISP) Family
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0031522 - cell envelope Sec protein transport complex
molecular_function GO:0005515 - protein binding
GO:0043021 - ribonucleoprotein complex binding
GO:0043022 - ribosome binding
GO:0046872 - metal ion binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0008270 - zinc ion binding
GO:0042802 - identical protein binding
GO:0070678 - preprotein binding
GO:0015462 - ATPase-coupled protein transmembrane transporter activity
biological_process GO:0015031 - protein transport
GO:0006605 - protein targeting
GO:0006886 - intracellular protein transport
GO:0017038 - protein import
GO:0065002 - intracellular protein transmembrane transport
GO:0043952 - protein transport by the Sec complex
GO:0061077 - chaperone-mediated protein folding
External database links:  
DIP:
 DIP-10840N
ECOCYC:
 SECA
ECOLIWIKI:
 b0098
INTERPRO:
 IPR036670
INTERPRO:
 IPR036266
INTERPRO:
 IPR027417
INTERPRO:
 IPR020937
INTERPRO:
 IPR014018
INTERPRO:
 IPR011130
INTERPRO:
 IPR011116
INTERPRO:
 IPR011115
INTERPRO:
 IPR004027
INTERPRO:
 IPR000185
MINT:
 P10408
MODBASE:
 P10408
PANTHER:
 PTHR30612
PDB:
 6GOX
PDB:
 3BXZ
PDB:
 2VDA
PDB:
 2FSH
PDB:
 2FSI
PDB:
 1TM6
PDB:
 2FSF
PDB:
 2FSG
PFAM:
 PF02810
PFAM:
 PF07516
PFAM:
 PF07517
PFAM:
 PF01043
PRIDE:
 P10408
PRINTS:
 PR00906
PRODB:
 PRO_000023923
PROSITE:
 PS01312
PROSITE:
 PS51196
REFSEQ:
 NP_414640
SMART:
 SM00958
SMART:
 SM00957
SMR:
 P10408
SWISSMODEL:
 P10408
UNIPROT:
 P10408


Operon      
Name: secMA-mutT         
Operon arrangement:
Transcription unit        Promoter
secMA-mutT


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_197 108025 forward nd [RS-EPT-CBR] [2]
  promoter TSS_198 108134 forward nd [RS-EPT-CBR] [2]
  promoter TSS_199 108703 forward nd [RS-EPT-CBR] [2]
  promoter TSS_200 108722 forward nd [RS-EPT-CBR] [2]
  promoter TSS_201 108821 forward nd [RS-EPT-CBR] [2]
  promoter TSS_202 109037 forward nd [RS-EPT-CBR] [2]
  promoter TSS_203 109101 forward nd [RS-EPT-CBR] [2]
  promoter TSS_204 109106 forward nd [RS-EPT-CBR] [2]
  promoter TSS_205 109414 forward nd [RS-EPT-CBR] [2]
  promoter TSS_206 109478 forward nd [RS-EPT-CBR] [2]
  promoter TSS_207 110273 forward nd [RS-EPT-CBR] [2]
  promoter TSS_208 110296 forward nd [RS-EPT-CBR] [2]
  promoter TSS_209 (cluster) 110304 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_210 110313 forward nd [RS-EPT-CBR] [2]
  promoter TSS_211 110324 forward nd [RS-EPT-CBR] [2]
  promoter TSS_212 110327 forward nd [RS-EPT-CBR] [2]
  promoter TSS_213 110338 forward nd [RS-EPT-CBR] [2]
  promoter TSS_214 110340 forward nd [RS-EPT-CBR] [2]
  promoter TSS_215 (cluster) 110667 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_216 (cluster) 110671 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_217 110684 forward nd [RS-EPT-CBR] [2]
  promoter TSS_218 110686 forward nd [RS-EPT-CBR] [2]
  promoter TSS_219 110690 forward nd [RS-EPT-CBR] [2]
  promoter TSS_220 (cluster) 110694 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_221 110709 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Schmidt MG., Rollo EE., Grodberg J., Oliver DB., 1988, Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli., J Bacteriol 170(8):3404-14

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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