RegulonDB

RegulonDB RegulonDB 10.8: Gene Form

bamE gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	bamE
Synonym(s):	ECK2613, EG10952, b2617, smpA, smqA
Genome position(nucleotides):	2753605 --> 2753946 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	49.42
External database links:

ASAP:	ABE-0008614
ECHOBASE:	EB0945
OU-MICROARRAY:	b2617
PortEco:	smpA
STRING:	511145.b2617
COLOMBOS:	bamE

Product

Name:

outer membrane protein assembly factor BamE

Synonym(s):

BamE, SmpA, SmqA, small membrane protein A

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

outer membrane

Molecular weight:

12.302

Isoelectric point:

8.726

Motif(s):

Type	Positions	Sequence
	40 -> 43	ANDV
	64 -> 64	M
	91 -> 91	L
	101 -> 107	LTNIDNK
	52 -> 58	QQQVAYA

Type	Positions	Sequence
	40 -> 43	ANDV
	64 -> 64	M
	91 -> 91	L
	101 -> 107	LTNIDNK
	52 -> 58	QQQVAYA
	66 -> 66	D
	1 -> 19	MRCKTLTAAAAVLLMLTAG
	32 -> 32	I
	34 -> 34	Q
	46 -> 46	I
	55 -> 55	V
	88 -> 88	Q
	90 -> 95	TLTLTF
	35 -> 35	G
	37 -> 37	Y
	38 -> 38	L
	78 -> 78	R
	72 -> 75	TWFY
	74 -> 74	F
	75 -> 75	Y
	95 -> 95	F
	59 -> 59	L
	73 -> 73	W
	76 -> 76	V
	20 -> 20	C
	36 -> 36	N
	68 -> 68	F
	93 -> 93	L

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0009279 - cell outer membrane GO:0019867 - outer membrane GO:0016020 - membrane GO:0045203 - integral component of cell outer membrane GO:1990063 - Bam protein complex
	molecular_function	GO:1901612 - cardiolipin binding GO:0005515 - protein binding GO:0030674 - protein-macromolecule adaptor activity GO:0042802 - identical protein binding
	biological_process	GO:0046677 - response to antibiotic GO:0043165 - Gram-negative-bacterium-type cell outer membrane assembly GO:0051205 - protein insertion into membrane

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[IHBCE] Inferred by a human based on computational evidence
[IPI] Inferred from physical interaction

Reference(s):

[1] Han L., et al., 2016
[2] Hart EM., et al., 2019
[3] Miczak A., et al., 1991
[4] Sabina J., et al., 2003
[5] Sklar JG., et al., 2007
[6] Tata M., et al., 2019
[7] Wei Y., et al., 2001

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MINT:

PANTHER:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

bamE

Operon arrangement:

Transcription unit	Promoter
bamE	bamEp

Transcriptional Regulation
	Display Regulation
Repressed by:	NsrR, CpxR

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2964	2752658	reverse	nd	[RS-EPT-CBR]	[8]
promoter	TSS_2965	2753488	forward	nd	[RS-EPT-CBR]	[8]
promoter	TSS_2966	2753555	forward	nd	[RS-EPT-CBR]	[8]
promoter	TSS_2967	2753588	forward	nd	[RS-EPT-CBR]	[8]
promoter	TSS_2968	2753594	forward	nd	[RS-EPT-CBR]	[8]
promoter	TSS_2969	2753599	forward	nd	[RS-EPT-CBR]	[8]
promoter	TSS_2970	2753605	forward	nd	[RS-EPT-CBR]	[8]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Han L., Zheng J., Wang Y., Yang X., Liu Y., Sun C., Cao B., Zhou H., Ni D., Lou J., Zhao Y., Huang Y., 2016, Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins., Nat Struct Mol Biol 23(3):192-6
[2] Hart EM., Gupta M., Wuhr M., Silhavy TJ., 2019, The Synthetic Phenotype of Δ bamB Δ bamE Double Mutants Results from a Lethal Jamming of the Bam Complex by the Lipoprotein RcsF., MBio 10(3)
[3] Miczak A., Chauhan AK., Apirion D., 1991, Two new genes located between 2758 and 2761 kilobase pairs on the Escherichia coli genome., J Bacteriol 173(11):3271-2
[4] Sabina J., Dover N., Templeton LJ., Smulski DR., Soll D., LaRossa RA., 2003, Interfering with different steps of protein synthesis explored by transcriptional profiling of Escherichia coli K-12., J Bacteriol 185(20):6158-70
[5] Sklar JG., Wu T., Gronenberg LS., Malinverni JC., Kahne D., Silhavy TJ., 2007, Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli., Proc Natl Acad Sci U S A 104(15):6400-5
[6] Tata M., Konovalova A., 2019, Improper Coordination of BamA and BamD Results in Bam Complex Jamming by a Lipoprotein Substrate., MBio 10(3)
[7] Wei Y., Lee JM., Richmond C., Blattner FR., Rafalski JA., LaRossa RA., 2001, High-density microarray-mediated gene expression profiling of Escherichia coli., J Bacteriol 183(2):545-56
[8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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