RegulonDB RegulonDB 10.8: Gene Form
   

sucD gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

sucB sucC sucD sdhX TSS_860 TSS_860 TSS_859 TSS_859 TSS_858 TSS_858 TSS_857 TSS_857 TSS_856 TSS_856 TSS_855 TSS_855 TSS_854 (cluster) TSS_854 (cluster) TSS_853 (cluster) TSS_853 (cluster) TSS_852 TSS_852 TSS_851 TSS_851

Gene      
Name: sucD    Texpresso search in the literature
Synonym(s): ECK0717, EG10982, b0729
Genome position(nucleotides): 764180 --> 765049 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.79
External database links:  
ASAP:
 ABE-0002485
CGSC:
 17842
ECHOBASE:
 EB0975
MIM:
 245400
OU-MICROARRAY:
 b0729
PortEco:
 sucD
STRING:
 511145.b0729
COLOMBOS: sucD


Product      
Name: succinyl-CoA synthetase subunit α
Synonym(s): alpha; subunit, SucD
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 29.777
Isoelectric point: 6.781
Motif(s):
 
Type Positions Sequence
151 -> 271 VSRSGTLTYEAVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEAAAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGV
17 -> 20 TGSQ
209 -> 209 E
2 -> 289 SILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGGTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPTLDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYEAVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEAAAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRSLADIGEALKTVLK
6 -> 99 DKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKGGTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEG

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.4 - TCA cycle
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0009361 - succinate-CoA ligase complex (ADP-forming)
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016874 - ligase activity
GO:0000166 - nucleotide binding
GO:0048037 - cofactor binding
GO:0004550 - nucleoside diphosphate kinase activity
GO:0004775 - succinate-CoA ligase (ADP-forming) activity
biological_process GO:0006099 - tricarboxylic acid cycle
GO:0009142 - nucleoside triphosphate biosynthetic process
GO:0046777 - protein autophosphorylation
GO:0006165 - nucleoside diphosphate phosphorylation
GO:0006105 - succinate metabolic process
GO:0006104 - succinyl-CoA metabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Freestone P., et al., 1995
[2] Luo GX., et al., 1992
External database links:  
DIP:
 DIP-31851N
ECOCYC:
 SUCCCOASYN-ALPHA
ECOLIWIKI:
 b0729
INTERPRO:
 IPR036291
INTERPRO:
 IPR016102
INTERPRO:
 IPR017440
INTERPRO:
 IPR033847
INTERPRO:
 IPR005810
INTERPRO:
 IPR003781
INTERPRO:
 IPR005811
MINT:
 MINT-1219451
PDB:
 2SCU
PDB:
 2NUA
PDB:
 2NU8
PDB:
 2NU7
PDB:
 2NU6
PDB:
 1SCU
PDB:
 1JLL
PDB:
 1JKJ
PDB:
 1CQJ
PDB:
 1CQI
PDB:
 2NU9
PFAM:
 PF02629
PFAM:
 PF00549
PRIDE:
 P0AGE9
PRINTS:
 PR01798
PRODB:
 PRO_000024005
PROSITE:
 PS01216
PROSITE:
 PS00399
REFSEQ:
 NP_415257
SMART:
 SM00881
SMR:
 P0AGE9
SWISSMODEL:
 P0AGE9
UNIPROT:
 P0AGE9


Operon      
Name: sdhCDAB-sucABCD-sdhX         
Operon arrangement:
Transcription unit        Promoter
sdhCDAB-sucABCD-sdhX
sdhDAB
sucAB
sucABCD


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, Fur, ArcA
Repressed by: FNR, IHF, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_851 761724 forward nd [RS-EPT-CBR] [3]
  promoter TSS_852 761850 forward nd [RS-EPT-CBR] [3]
  promoter TSS_853 (cluster) 762415 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [3]
  promoter TSS_854 (cluster) 762749 forward For this promoter, there
Read more > 		[RS-EPT-CBR] [3]
  promoter TSS_855 762984 forward nd [RS-EPT-CBR] [3]
  promoter TSS_856 762991 forward nd [RS-EPT-CBR] [3]
  promoter TSS_857 763299 forward nd [RS-EPT-CBR] [3]
  promoter TSS_858 764187 forward nd [RS-EPT-CBR] [3]
  promoter TSS_859 764211 forward nd [RS-EPT-CBR] [3]
  promoter TSS_860 764423 forward nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Freestone P., Grant S., Toth I., Norris V., 1995, Identification of phosphoproteins in Escherichia coli., Mol Microbiol 15(3):573-80

 [2] Luo GX., Nishimura JS., 1992, Adenosine 5'-tetraphosphate is synthesized by the histidine alpha 142----asparagine mutant of Escherichia coli succinyl-CoA synthetase., J Biol Chem 267(14):9516-20

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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