RegulonDB RegulonDB 10.8: Gene Form
   

sulA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ompA sxy sulA yccS CRP-Sxy LexA RcdA RcdA CRP RseX MicA anti-anti-terminator anti-terminator terminator TSS_1285 TSS_1285 sxyp sxyp TSS_1283 TSS_1283 sulAp sulAp TSS_1282 TSS_1282 ompAp1 ompAp1 ompAp2 ompAp2 TSS_1280 TSS_1280 TSS_1279 TSS_1279 TSS_1278 TSS_1278 TSS_1277 TSS_1277 TSS_1276 TSS_1276 TSS_1275 TSS_1275 TSS_1274 TSS_1274 TSS_1273 TSS_1273 TSS_1272 TSS_1272 TSS_1271 TSS_1271 TSS_1270 TSS_1270 TSS_1269 TSS_1269 TSS_1268 TSS_1268 TSS_1267 TSS_1267 TSS_1266 TSS_1266 TSS_1265 TSS_1265 TSS_1264 TSS_1264 TSS_1263 TSS_1263 TSS_1262 TSS_1262 TSS_1261 TSS_1261

Gene      
Name: sulA    Texpresso search in the literature
Synonym(s): ECK0949, EG10984, b0958, sfiA, suf
Genome position(nucleotides): 1020410 <-- 1020919 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
48.24
External database links:  
ASAP:
ABE-0003244
CGSC:
144
ECHOBASE:
EB0977
OU-MICROARRAY:
b0958
PortEco:
sulA
STRING:
511145.b0958
COLOMBOS: sulA


Product      
Name: cell division inhibitor SulA
Synonym(s): SOS cell division inhibitor, SfiA, Suf, SulA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 18.801
Isoelectric point: 7.161
Motif(s):
 
Type Positions Sequence
39 -> 39 R
72 -> 72 K
83 -> 83 L
163 -> 163 I
76 -> 76 E

 

Classification:
Multifun Terms (GenProtEC)  
  3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional
  5 - cell processes --> 5.1 - cell division
  5 - cell processes --> 5.8 - SOS response
Gene Ontology Terms (GO)  
cellular_component GO:0005886 - plasma membrane
molecular_function GO:0005515 - protein binding
biological_process GO:0051301 - cell division
GO:0006974 - cellular response to DNA damage stimulus
GO:0007049 - cell cycle
GO:0009432 - SOS response
GO:0000917 - division septum assembly
GO:0051782 - negative regulation of cell division
GO:0032466 - negative regulation of cytokinesis
GO:0032272 - negative regulation of protein polymerization
GO:0034260 - negative regulation of GTPase activity
GO:2000245 - negative regulation of FtsZ-dependent cytokinesis
Note(s): Note(s): ...[more].
Evidence: [IDA] Inferred from direct assay
Reference(s): [1] Aksenov SV. 1999
[2] Bi E., et al., 1990
[3] Burton P., et al., 1983
[4] Burton P., et al., 1983
[5] Caldeira de Araujo A., et al., 1986
[6] Canceill D., et al., 1990
[7] Cegielska A., et al., 1980
[8] Cegielska A., et al., 1985
[9] D'Ari R., et al., 1982
[10] Freudl R., et al., 1987
[11] Gottesman S., et al., 1981
[12] Holland IB., et al., 1985
[13] Huisman O., et al., 1983
[14] Huisman O., et al., 1982
[15] Huisman O., et al., 1983
[16] Jaffe A., et al., 1985
[17] Johnson BF. 1977
[18] Koukalova B., et al., 1986
[19] Kwon AR., et al., 2004
[20] Lee YY., et al., 2003
[21] Mukherjee A., et al., 1998
[22] Nazir A., et al., 2016
[23] Preobrajenskaya O., et al., 1994
[24] Quillardet P., et al., 1984
[25] Quillardet P., et al., 1982
[26] Schoemaker JM., et al., 1984
[27] Smirnova GV., et al., 1994
[28] Trempy JE., et al., 1989
[29] Van Melderen L., et al., 1999
[30] Vasil'eva SV., et al., 2003
[31] el-Hajj HH., et al., 1988
External database links:  
DIP:
DIP-10945N
ECOCYC:
EG10984-MONOMER
ECOLIWIKI:
b0958
INTERPRO:
IPR027417
INTERPRO:
IPR004596
MODBASE:
P0AFZ5
PFAM:
PF03846
PRIDE:
P0AFZ5
PRODB:
PRO_000024013
REFSEQ:
NP_415478
SMR:
P0AFZ5
UNIPROT:
P0AFZ5


Operon      
Name: sulA         
Operon arrangement:
Transcription unit        Promoter
sulA


Transcriptional Regulation      
Display Regulation             
Repressed by: LexA, RcdA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1261 1019168 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1262 1019186 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1263 1019190 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1264 1019205 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1265 1019248 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1266 1019250 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1267 1019362 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1268 1019382 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1269 1019401 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1270 1019732 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1271 1019778 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1272 1019848 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1273 1019850 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1274 1019917 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1275 1019929 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1276 1019931 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1277 1019980 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1278 1020044 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1279 1020049 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1280 1020184 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1282 1020205 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1283 1020949 reverse nd [RS-EPT-CBR] [32]
  promoter TSS_1285 1021378 forward nd [RS-EPT-CBR] [32]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Aksenov SV., 1999, Induction of the SOS Response in Ultraviolet-Irradiated Escherichia coli Analyzed by Dynamics of LexA, RecA and SulA Proteins., J Biol Phys 25(2-3):263-77

 [2] Bi E., Lutkenhaus J., 1990, Interaction between the min locus and ftsZ., J Bacteriol 172(10):5610-6

 [3] Burton P., Holland IB., 1983, Two pathways of division inhibition in UV-irradiated E. coli., Mol Gen Genet 190(1):128-32

 [4] Burton P., Holland IB., 1983, Two pathways of division inhibition in UV-irradiated E. coli., Mol Gen Genet 190(2):309-14

 [5] Caldeira de Araujo A., Favre A., 1986, Near ultraviolet DNA damage induces the SOS responses in Escherichia coli., EMBO J 5(1):175-9

 [6] Canceill D., Dervyn E., Huisman O., 1990, Proteolysis and modulation of the activity of the cell division inhibitor SulA in Escherichia coli lon mutants., J Bacteriol 172(12):7297-300

 [7] Cegielska A., Kupryszewski G., Taylor A., 1980, Specific response of Escherichia coli K12 sfiA mutants to the presence of ethyl ester of N-alpha-palmitoyl-L-lysyl-L-lysine dihydrochloride (PLL) in a nutrient medium., Mol Gen Genet 180(2):483-5

 [8] Cegielska A., Taylor A., 1985, The sfiA11 mutation prevents filamentation in a response to cell wall damage only in a recA+ genetic background., Mol Gen Genet 201(3):537-42

 [9] D'Ari R., Huisman O., 1982, DNA replication and indirect induction of the SOS response in Escherichia coli., Biochimie 64(8-9):623-7

 [10] Freudl R., Braun G., Honore N., Cole ST., 1987, Evolution of the enterobacterial sulA gene: a component of the SOS system encoding an inhibitor of cell division., Gene 52(1):31-40

 [11] Gottesman S., Halpern E., Trisler P., 1981, Role of sulA and sulB in filamentation by lon mutants of Escherichia coli K-12., J Bacteriol 148(1):265-73

 [12] Holland IB., Jones C., 1985, The role of the FtsZ protein (SfiB) in UV-induced division inhibition and in the normal Escherichia coli cell division cycle., Ann Inst Pasteur Microbiol 136A(1):165-71

 [13] Huisman O., D'Ari R., 1983, Effect of suppressors of SOS-mediated filamentation on sfiA operon expression in Escherichia coli., J Bacteriol 153(1):169-75

 [14] Huisman O., D'Ari R., Casaregola S., 1982, How Escherichia coli sets different basal levels in SOS operons., Biochimie 64(8-9):709-12

 [15] Huisman O., Jacques M., D'ari R., Caro L., 1983, Role of the sfiA-dependent cell division regulation system in Escherichia coli., J Bacteriol 153(2):1072-4

 [16] Jaffe A., D'Ari R., 1985, Regulation of chromosome segregation in Escherichia coli., Ann Inst Pasteur Microbiol 136A(1):159-64

 [17] Johnson BF., 1977, Fine structure mapping and properties of mutations suppressing the lon mutation in Escherichia coli K-12 and B strains., Genet Res 30(3):273-86

 [18] Koukalova B., Soska J., Kuhrova V., Reich J., 1986, SOS induction of the gene sulA is partly inhibited in Escherichia coli K12 cells overproducing the RecA protein., Mutat Res 175(1):17-21

 [19] Kwon AR., Trame CB., McKay DB., 2004, Kinetics of protein substrate degradation by HslUV., J Struct Biol 146(1-2):141-7

 [20] Lee YY., Chang CF., Kuo CL., Chen MC., Yu CH., Lin PI., Wu WF., 2003, Subunit oligomerization and substrate recognition of the Escherichia coli ClpYQ (HslUV) protease implicated by in vivo protein-protein interactions in the yeast two-hybrid system., J Bacteriol 185(8):2393-401

 [21] Mukherjee A., Cao C., Lutkenhaus J., 1998, Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli., Proc Natl Acad Sci U S A 95(6):2885-90

 [22] Nazir A., Harinarayanan R., 2016, Inactivation of Cell Division Protein FtsZ by SulA Makes Lon Indispensable for the Viability of a ppGpp0 Strain of Escherichia coli., J Bacteriol 198(4):688-700

 [23] Preobrajenskaya O., Boullard A., Boubrik F., Schnarr M., Rouviere-Yaniv J., 1994, The protein HU can displace the LexA repressor from its DNA-binding sites., Mol Microbiol 13(3):459-67

 [24] Quillardet P., Hofnung M., 1984, Induction by UV light of the SOS function sfiA in Escherichia coli strains deficient or proficient in excision repair., J Bacteriol 157(1):35-8

 [25] Quillardet P., Huisman O., D'Ari R., Hofnung M., 1982, SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity., Proc Natl Acad Sci U S A 79(19):5971-5

 [26] Schoemaker JM., Gayda RC., Markovitz A., 1984, Regulation of cell division in Escherichia coli: SOS induction and cellular location of the sulA protein, a key to lon-associated filamentation and death., J Bacteriol 158(2):551-61

 [27] Smirnova GV., Oktyabrsky ON., Moshonkina EV., Zakirova NV., 1994, Induction of the alkylation-inducible aidB gene of Escherichia coli by cytoplasmic acidification and N-ethylmaleimide., Mutat Res 314(1):51-6

 [28] Trempy JE., Gottesman S., 1989, Alp, a suppressor of lon protease mutants in Escherichia coli., J Bacteriol 171(6):3348-53

 [29] Van Melderen L., Gottesman S., 1999, Substrate sequestration by a proteolytically inactive Lon mutant., Proc Natl Acad Sci U S A 96(11):6064-71

 [30] Vasil'eva SV., Makhova EV., 2003, [Heat shock inhibits the induced expression of the SOS genes and SoxRS regulons in Escherichia coli], Genetika 39(8):1033-8

 [31] el-Hajj HH., Zhang H., Weiss B., 1988, Lethality of a dut (deoxyuridine triphosphatase) mutation in Escherichia coli., J Bacteriol 170(3):1069-75

 [32] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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