RegulonDB RegulonDB 10.7: Gene Form
   

tolA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tolB tolA tolR terminator anti-terminator anti-anti-terminator TSS_894 TSS_894 TSS_893 TSS_893 TSS_892 TSS_892 TSS_891 TSS_891 tolBp3 tolBp3 tolBp6 tolBp6 TSS_890 (cluster) TSS_890 (cluster)

Gene      
Name: tolA    Texpresso search in the literature
Synonym(s): ECK0728, EG11007, b0739, cim, excC, lky, tol-2
Genome position(nucleotides): 776342 --> 777607 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.29
External database links:  
ASAP:
ABE-0002515
CGSC:
99
ECHOBASE:
EB1000
OU-MICROARRAY:
b0739
PORTECO:
tolA
REGULONDB:
b0739
STRING:
511145.b0739
M3D: tolA
COLOMBOS: tolA
PortEco: b0739


Product      
Name: Tol-Pal system protein TolA
Synonym(s): Cim, ExcC, Lky, TolA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 43.157
Isoelectric point: 9.765
Motif(s):
 
Type Positions Sequence
26 -> 26 F
251 -> 255 EKAAA
241 -> 245 EKAAA
224 -> 292 EKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAA
246 -> 250 DKKAA

 

Classification:
Multifun Terms (GenProtEC)  
  6 - cell structure --> 6.1 - membrane
  8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
  8 - extrachromosomal --> 8.4 - colicin related
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0032153 - cell division site
molecular_function GO:0097718 - disordered domain specific binding
GO:0019904 - protein domain specific binding
GO:0005515 - protein binding
GO:0046790 - virion binding
GO:0019534 - toxin transmembrane transporter activity
biological_process GO:0051301 - cell division
GO:0007049 - cell cycle
GO:0017038 - protein import
GO:0043213 - bacteriocin transport
GO:0046718 - viral entry into host cell
GO:1901998 - toxin transport
GO:0071237 - cellular response to bacteriocin
Note(s): Note(s): ...[more].
Reference(s): [1] Anderluh G., et al., 2003
[2] Bouveret E., et al., 1998
[3] Click EM., et al., 1997
[4] Deprez C., et al., 2002
[5] Germon P., et al., 2001
[6] Gokce I., et al., 2000
[7] Hecht O., et al., 2009
[8] Levengood-Freyermuth SK., et al., 1993
[9] McBroom AJ., et al., 2006
[10] Raggett EM., et al., 1998
[11] Schendel SL., et al., 1997
External database links:  
DIP:
DIP-11005N
ECOCYC:
EG11007-MONOMER
ECOLIWIKI:
b0739
INTERPRO:
IPR014161
MINT:
P19934
MODBASE:
P19934
PDB:
3QDR
PDB:
3QDP
PDB:
1TOL
PDB:
1S62
PFAM:
PF06519
PRIDE:
P19934
PRODB:
PRO_000024078
PROTEINMODELPORTAL:
P19934
REFSEQ:
NP_415267
SMR:
P19934
UNIPROT:
P19934


Operon      
Name: ybgC-tolQRA         
Operon arrangement:
Transcription unit        Promoter
ybgC-tolQRA
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_890 (cluster) 777229 forward For this promoter, there
Read more >
[RS-EPT-CBR] [12]
  promoter tolBp6 777537 forward Similarity to the consensus
Read more >
[ICWHO] [13]
  promoter tolBp3 777560 forward Similarity to the consensus
Read more >
[ICWHO] [13]
  promoter TSS_891 777769 forward nd [RS-EPT-CBR] [12]
  promoter TSS_892 778129 forward nd [RS-EPT-CBR] [12]
  promoter TSS_893 778824 forward nd [RS-EPT-CBR] [12]
  promoter TSS_894 778979 forward nd [RS-EPT-CBR] [12]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Anderluh G., Hong Q., Boetzel R., MacDonald C., Moore GR., Virden R., Lakey JH., 2003, Concerted folding and binding of a flexible colicin domain to its periplasmic receptor TolA., J Biol Chem 278(24):21860-8

 [2] Bouveret E., Rigal A., Lazdunski C., Benedetti H., 1998, Distinct regions of the colicin A translocation domain are involved in the interaction with TolA and TolB proteins upon import into Escherichia coli., Mol Microbiol 27(1):143-57

 [3] Click EM., Webster RE., 1997, Filamentous phage infection: required interactions with the TolA protein., J Bacteriol 179(20):6464-71

 [4] Deprez C., Blanchard L., Guerlesquin F., Gavioli M., Simorre JP., Lazdunski C., Marion D., Lloubes R., 2002, Macromolecular import into Escherichia coli: the TolA C-terminal domain changes conformation when interacting with the colicin A toxin., Biochemistry 41(8):2589-98

 [5] Germon P., Ray MC., Vianney A., Lazzaroni JC., 2001, Energy-dependent conformational change in the TolA protein of Escherichia coli involves its N-terminal domain, TolQ, and TolR., J Bacteriol 183(14):4110-4

 [6] Gokce I., Raggett EM., Hong Q., Virden R., Cooper A., Lakey JH., 2000, The TolA-recognition site of colicin N. ITC, SPR and stopped-flow fluorescence define a crucial 27-residue segment., J Mol Biol 304(4):621-32

 [7] Hecht O., Ridley H., Lakey JH., Moore GR., 2009, A common interaction for the entry of colicin N and filamentous phage into Escherichia coli., J Mol Biol 388(4):880-93

 [8] Levengood-Freyermuth SK., Click EM., Webster RE., 1993, Role of the carboxyl-terminal domain of TolA in protein import and integrity of the outer membrane., J Bacteriol 175(1):222-8

 [9] McBroom AJ., Johnson AP., Vemulapalli S., Kuehn MJ., 2006, Outer membrane vesicle production by Escherichia coli is independent of membrane instability., J Bacteriol 188(15):5385-92

 [10] Raggett EM., Bainbridge G., Evans LJ., Cooper A., Lakey JH., 1998, Discovery of critical Tol A-binding residues in the bactericidal toxin colicin N: a biophysical approach., Mol Microbiol 28(6):1335-43

 [11] Schendel SL., Click EM., Webster RE., Cramer WA., 1997, The TolA protein interacts with colicin E1 differently than with other group A colicins., J Bacteriol 179(11):3683-90

 [12] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [13] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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