RegulonDB RegulonDB 10.7: Gene Form
   

tonB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tonB yciA yciI Fur Fur Fur Fur TSS_1684 TSS_1684 tonBp tonBp yciIp yciIp

Gene      
Name: tonB    Texpresso search in the literature
Synonym(s): ECK1246, EG11012, T1rec, b1252, exbA
Genome position(nucleotides): 1311089 --> 1311808 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.44
External database links:  
ASAP:
ABE-0004196
CGSC:
90
ECHOBASE:
EB1005
OU-MICROARRAY:
b1252
PORTECO:
tonB
REGULONDB:
b1252
STRING:
511145.b1252
M3D: tonB
COLOMBOS: tonB
PortEco: b1252


Product      
Name: Ton complex subunit TonB
Synonym(s): ExbA, TonB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,periplasmic space
Molecular weight: 26.094
Isoelectric point: 10.277
Motif(s):
 
Type Positions Sequence
34 -> 154 QVIELPAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPR
78 -> 79 IP
80 -> 81 EP
93 -> 94 KP
95 -> 96 KP

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.4 - energy production/transport
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.C - Ion-gradient-driven energizers
  5 - cell processes --> 5.5 - adaptations --> 5.5.7 - Fe aquisition
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
GO:0071575 - integral component of external side of plasma membrane
GO:0098797 - plasma membrane protein complex
molecular_function GO:0019904 - protein domain specific binding
GO:0005515 - protein binding
GO:0015343 - siderophore transmembrane transporter activity
GO:0031992 - energy transducer activity
biological_process GO:0098002 - receptor-mediated bacteriophage irreversible attachment to host cell
GO:0015031 - protein transport
GO:0015889 - cobalamin transport
GO:0015891 - siderophore transport
GO:0043213 - bacteriocin transport
GO:0055085 - transmembrane transport
GO:0042914 - colicin transport
GO:0044718 - siderophore transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Chu BC., et al., 2007
[2] Ghosh J., et al., 2004
[3] Ghosh J., et al., 2005
[4] Gudmundsdottir A., et al., 1989
[5] Hantke K., et al., 1975
[6] Higgs PI., et al., 2002
[7] Howard SP., et al., 2001
[8] Kadner RJ., et al., 1995
[9] Kadner RJ., et al., 1978
[10] Karlsson M., et al., 1993
[11] Khursigara CM., et al., 2005
[12] Killmann H., et al., 1994
[13] Kodding J., et al., 2004
[14] Koedding J., et al., 2004
[15] Larsen RA., et al., 1997
[16] Larsen RA., et al., 2003
[17] Larsen RA., et al., 1996
[18] Larsen RA., et al., 2001
[19] Larsen RA., et al., 1994
[20] Lill Y., et al., 2016
[21] Moeck GS., et al., 1997
[22] Postle K. 2007
[23] Skare JT., et al., 1991
[24] Traub I., et al., 1993
[25] Wang CC., et al., 1971
[26] Zhai YF., et al., 2003
External database links:  
DIP:
DIP-48111N
DISPROT:
DP00043
ECOCYC:
EG11012-MONOMER
ECOLIWIKI:
b1252
INTERPRO:
IPR037682
INTERPRO:
IPR006260
INTERPRO:
IPR003538
MODBASE:
P02929
PDB:
2GSK
PDB:
2GRX
PDB:
1XX3
PDB:
1U07
PDB:
1QXX
PDB:
1IHR
PFAM:
PF03544
PRIDE:
P02929
PRINTS:
PR01374
PRODB:
PRO_000024083
PROTEINMODELPORTAL:
P02929
REFSEQ:
NP_415768
SMR:
P02929
UNIPROT:
P02929


Operon      
Name: tonB         
Operon arrangement:
Transcription unit        Promoter
tonB


Transcriptional Regulation      
Display Regulation             
Activated by: Fur
Repressed by: Fur


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1684 1311057 forward nd [RS-EPT-CBR] [27]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Chu BC., Peacock RS., Vogel HJ., 2007, Bioinformatic analysis of the TonB protein family., Biometals 20(3-4):467-83

 [2] Ghosh J., Postle K., 2004, Evidence for dynamic clustering of carboxy-terminal aromatic amino acids in TonB-dependent energy transduction., Mol Microbiol 51(1):203-13

 [3] Ghosh J., Postle K., 2005, Disulphide trapping of an in vivo energy-dependent conformation of Escherichia coli TonB protein., Mol Microbiol 55(1):276-88

 [4] Gudmundsdottir A., Bell PE., Lundrigan MD., Bradbeer C., Kadner RJ., 1989, Point mutations in a conserved region (TonB box) of Escherichia coli outer membrane protein BtuB affect vitamin B12 transport., J Bacteriol 171(12):6526-33

 [5] Hantke K., Braun V., 1975, Membrane receptor dependent iron transport in Escherichia coli., FEBS Lett 49(3):301-5

 [6] Higgs PI., Letain TE., Merriam KK., Burke NS., Park H., Kang C., Postle K., 2002, TonB interacts with nonreceptor proteins in the outer membrane of Escherichia coli., J Bacteriol 184(6):1640-8

 [7] Howard SP., Herrmann C., Stratilo CW., Braun V., 2001, In vivo synthesis of the periplasmic domain of TonB inhibits transport through the FecA and FhuA iron siderophore transporters of Escherichia coli., J Bacteriol 183(20):5885-95

 [8] Kadner RJ., Heller KJ., 1995, Mutual inhibition of cobalamin and siderophore uptake systems suggests their competition for TonB function., J Bacteriol 177(17):4829-35

 [9] Kadner RJ., McElhaney G., 1978, Outer membrane-dependent transport systems in Escherichia coli: turnover of TonB function., J Bacteriol 134(3):1020-9

 [10] Karlsson M., Hannavy K., Higgins CF., 1993, A sequence-specific function for the N-terminal signal-like sequence of the TonB protein., Mol Microbiol 8(2):379-88

 [11] Khursigara CM., De Crescenzo G., Pawelek PD., Coulton JW., 2005, Deletion of the proline-rich region of TonB disrupts formation of a 2:1 complex with FhuA, an outer membrane receptor of Escherichia coli., Protein Sci 14(5):1266-73

 [12] Killmann H., Braun V., 1994, Energy-dependent receptor activities of Escherichia coli K-12: mutated TonB proteins alter FhuA receptor activities to phages T5, T1, phi 80 and to colicin M., FEMS Microbiol Lett 119(1-2):71-6

 [13] Kodding J., Killig F., Polzer P., Howard SP., Diederichs K., Welte W., 2004, Crystal structure of a 92-residue C-terminal fragment of TonB from Escherichia coli reveals significant conformational changes compared to structures of smaller TonB fragments., J Biol Chem 280(4):3022-8

 [14] Koedding J., Howard P., Kaufmann L., Polzer P., Lustig A., Welte W., 2004, Dimerization of TonB is not essential for its binding to the outer membrane siderophore receptor FhuA of Escherichia coli., J Biol Chem 279(11):9978-86

 [15] Larsen RA., Foster-Hartnett D., McIntosh MA., Postle K., 1997, Regions of Escherichia coli TonB and FepA proteins essential for in vivo physical interactions., J Bacteriol 179(10):3213-21

 [16] Larsen RA., Letain TE., Postle K., 2003, In vivo evidence of TonB shuttling between the cytoplasmic and outer membrane in Escherichia coli., Mol Microbiol 49(1):211-8

 [17] Larsen RA., Myers PS., Skare JT., Seachord CL., Darveau RP., Postle K., 1996, Identification of TonB homologs in the family Enterobacteriaceae and evidence for conservation of TonB-dependent energy transduction complexes., J Bacteriol 178(5):1363-73

 [18] Larsen RA., Postle K., 2001, Conserved residues Ser(16) and His(20) and their relative positioning are essential for TonB activity, cross-linking of TonB with ExbB, and the ability of TonB to respond to proton motive force., J Biol Chem 276(11):8111-7

 [19] Larsen RA., Thomas MG., Wood GE., Postle K., 1994, Partial suppression of an Escherichia coli TonB transmembrane domain mutation (delta V17) by a missense mutation in ExbB., Mol Microbiol 13(4):627-40

 [20] Lill Y., Jordan LD., Smallwood CR., Newton SM., Lill MA., Klebba PE., Ritchie K., 2016, Confined Mobility of TonB and FepA in Escherichia coli Membranes., PLoS One 11(12):e0160862

 [21] Moeck GS., Coulton JW., Postle K., 1997, Cell envelope signaling in Escherichia coli. Ligand binding to the ferrichrome-iron receptor fhua promotes interaction with the energy-transducing protein TonB., J Biol Chem 272(45):28391-7

 [22] Postle K., 2007, TonB system, in vivo assays and characterization., Methods Enzymol 422:245-69

 [23] Skare JT., Postle K., 1991, Evidence for a TonB-dependent energy transduction complex in Escherichia coli., Mol Microbiol 5(12):2883-90

 [24] Traub I., Gaisser S., Braun V., 1993, Activity domains of the TonB protein., Mol Microbiol 8(2):409-23

 [25] Wang CC., Newton A., 1971, An additional step in the transport of iron defined by the tonB locus of Escherichia coli., J Biol Chem 246(7):2147-51

 [26] Zhai YF., Heijne W., Saier MH., 2003, Molecular modeling of the bacterial outer membrane receptor energizer, ExbBD/TonB, based on homology with the flagellar motor, MotAB., Biochim Biophys Acta 1614(2):201-10

 [27] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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