RegulonDB RegulonDB 8.6: Gene Form
   

tufB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

tufB secE thrT terminator anti-terminator anti-anti-terminator TSS_4353 TSS_4353 TSS_4352 TSS_4352 TSS_4351 (cluster) TSS_4351 (cluster) TSS_4350 (cluster) TSS_4350 (cluster) TSS_4349 (cluster) TSS_4349 (cluster) TSS_4348 (cluster) TSS_4348 (cluster) TSS_4347 TSS_4347 TSS_4346 (cluster) TSS_4346 (cluster) TSS_4345 TSS_4345 secEp secEp TSS_4344 TSS_4344 TSS_4343 TSS_4343 TSS_4342 TSS_4342 TSS_4341 TSS_4341 TSS_4340 (cluster) TSS_4340 (cluster) TSS_4339 (cluster) TSS_4339 (cluster) TSS_4338 TSS_4338 TSS_4337 (cluster) TSS_4337 (cluster) TSS_4336 TSS_4336 TSS_4335 (cluster) TSS_4335 (cluster) TSS_4334 TSS_4334 TSS_4333 TSS_4333 TSS_4332 TSS_4332 TSS_4331 TSS_4331 TSS_4330 TSS_4330 TSS_4329 TSS_4329 TSS_4328 TSS_4328 TSS_4327 TSS_4327 TSS_4326 (cluster) TSS_4326 (cluster) TSS_4325 TSS_4325 TSS_4324 TSS_4324 TSS_4323 TSS_4323 TSS_4322 TSS_4322 TSS_4321 TSS_4321 TSS_4320 TSS_4320 TSS_4319 (cluster) TSS_4319 (cluster)

Gene      
Name: tufB    Texpresso search in the literature
Synonym(s): kirT, pulT
Genome position(nucleotides): 4173967 --> 4175151 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.42
External database links:  
ECOCYC:
EG11037
OU-MICROARRAY:
b3980
M3D: tufB
COLOMBOS: tufB
PortEco: b3980


Product      
Name: elongation factor Tu
Synonym(s): EF-Tu, KirT, PulT, TufB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,inner membrane
Molecular weight: 43.314
Isoelectric point: 5.14
Motif(s):
 
Type Positions Sequence
19 -> 26 GHVDHGKT
136 -> 139 NKCD
2 -> 394 SKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS
137 -> 137 K
349 -> 349 E

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.2 - translation
  5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
Gene Ontology Terms (GO)  
cellular_component GO:0005622 - intracellular
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
GO:0005886 - plasma membrane
molecular_function GO:0003746 - translation elongation factor activity
GO:0000166 - nucleotide binding
GO:0003924 - GTPase activity
GO:0005525 - GTP binding
biological_process GO:0006412 - translation
GO:0006414 - translational elongation
GO:0006970 - response to osmotic stress
GO:0046677 - response to antibiotic
GO:0006184 - GTP catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Weijland A., et al., 1992
External database links:  
ECOCYC:
EG11037-MONOMER
GENPROTEC:
b3980
PDB:
1EFU
PDB:
1LS2
PDB:
1OB2
PDB:
2BVN
PDB:
3FIH
PDB:
3IZV
PDB:
3IZW
PDB:
3MMP
PDB:
1EFM
PDB:
1DG1
PDB:
1QZD


Operon      
Name: thrU-tyrU-glyT-thrT-tufB         
Operon arrangement:
Transcription unit        Promoter
thrU-tyrU-glyT-thrT-tufB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4319 (cluster) 4173892 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4320 4173897 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4321 4173906 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4322 4173940 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4323 4173943 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4324 4173949 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4325 4173951 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4326 (cluster) 4173981 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4327 4173991 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4328 4174139 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4329 4174348 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4330 4174355 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4331 4174452 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4332 4174466 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4333 4174468 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4334 4174475 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4335 (cluster) 4174609 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4336 4174614 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4337 (cluster) 4174616 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4338 4174620 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4339 (cluster) 4174682 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4340 (cluster) 4174692 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4341 4174727 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4342 4174750 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4343 4174762 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4344 4174998 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4345 4175236 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4346 (cluster) 4175266 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4347 4175269 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4348 (cluster) 4175301 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4349 (cluster) 4175318 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4350 (cluster) 4175324 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4351 (cluster) 4175352 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_4352 4175363 forward nd [RS-EPT-CBR] [2]
  promoter TSS_4353 4175466 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Weijland A., Harmark K., Cool RH., Anborgh PH., Parmeggiani A., 1992, Elongation factor Tu: a molecular switch in protein biosynthesis., Mol Microbiol. 6(6):683-8

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu¿¿iz-Rascado L, Garc¿¿a-Sotelo JS, Weiss V, Solano-Lira H, Mart¿¿nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern¿¿ndez S, Alquicira-Hern¿¿ndez K, L¿¿pez-Fuentes A, Porr¿¿n-Sotelo L, Huerta AM, Bonavides-Mart¿¿nez C, Balderas-Mart¿¿nez YI, Pannier L, Olvera M, Labastida A, Jim¿¿nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch¿¿vez V, Hern¿¿ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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