RegulonDB

RegulonDB RegulonDB 10.8: Gene Form

mazF gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	mazF
Synonym(s):	ECK2776, EG11249, b2782, chpA, chpAK
Genome position(nucleotides):	2910756 <-- 2911091 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	46.13
External database links:

ASAP:	ABE-0009119
CGSC:	33287
ECHOBASE:	EB1229
OU-MICROARRAY:	b2782
PortEco:	chpA
STRING:	511145.b2782
COLOMBOS:	mazF

Product

Name:

endoribonuclease toxin MazF

Synonym(s):

ChpA, ChpAK, MazF, MazF toxin of the MazF-MazE toxin-antitoxin system that exhibits ribonuclease activity

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

12.098

Isoelectric point:

8.478

Motif(s):

Type	Positions	Sequence
	10 -> 109	GDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLIKAKINVL
	17 -> 28	FDPTKGSEQAGH
	53 -> 61	TQSKGYPFE
	24 -> 24	E
	28 -> 28	H

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.4 - RNA degradation
		2 - information transfer --> 2.3 - protein related --> 2.3.2 - translation
		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.1 - translation attenuation and efficiency
		5 - cell processes --> 5.10 - defense/survival
		5 - cell processes --> 5.5 - adaptations --> 5.5.3 - starvation
		5 - cell processes --> 5.5 - adaptations --> 5.5.6 - other (mechanical, nutritional, oxidative stress)
		5 - cell processes --> 5.6 - protection --> 5.6.3 - cell killing
		5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:0032991 - protein-containing complex
	molecular_function	GO:0003677 - DNA binding GO:0004518 - nuclease activity GO:0004519 - endonuclease activity GO:0005515 - protein binding GO:0016787 - hydrolase activity GO:0003723 - RNA binding GO:0004521 - endoribonuclease activity GO:0042803 - protein homodimerization activity GO:0044877 - protein-containing complex binding
	biological_process	GO:0043068 - positive regulation of programmed cell death GO:0006417 - regulation of translation GO:0009372 - quorum sensing GO:0006402 - mRNA catabolic process GO:0030308 - negative regulation of cell growth GO:0016075 - rRNA catabolic process GO:0051607 - defense response to virus GO:0090305 - nucleic acid phosphodiester bond hydrolysis GO:0090502 - RNA phosphodiester bond hydrolysis, endonucleolytic

Note(s):

Note(s): ...[more].

External database links:

DISPROT:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PDB:

PDB:

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PDB:

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PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

SWISSMODEL:

UNIPROT:

Operon

Name:

relA-mazEFG

Operon arrangement:

Transcription unit	Promoter
mazEFG	mazEp2
mazEFG	mazEp1
relA	relAp3
relA-mazEF	relAp1
relA-mazEF	relAp2
relA	relAp4

Transcriptional Regulation

Display Regulation

Activated by:

CRP, H-NS, Fis

Repressed by:

HipAB, MazE, MazE-MazF, HipB

Growth Conditions:

[1]	C: Escherichia coli\| plasmid pALS13 (not induced truncated relA) mutant\| LB medium\| 37.0 C\| mid exponential phase E: Escherichia coli\| plasmid pALS13 (IPTG-induced truncated relA) mutant\| LB medium\| 37.0 C\| mid exponential phase

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	mazGp4	2910860	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.735. P-value: 5.947e-5 Read more >	[ICWHO]	[1]
promoter	TSS_3085	2913146	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_3086	2913158	reverse	nd	[RS-EPT-CBR]	[2]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

RegulonDB