RegulonDB

Gene
Name:	nfsA
Synonym(s):	ECK0842, EG11261, b0851, mda18, mdaA, ybjB
Genome position(nucleotides):	891184 --> 891906 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.56
External database links:

ASAP:	ABE-0002900
ECHOBASE:	EB1241
OU-MICROARRAY:	b0851
PortEco:	nfsA
STRING:	511145.b0851
COLOMBOS:	nfsA

Product

Name:

NADPH-dependent nitroreductase NfsA

Synonym(s):

MdaA, NfsA, YbjB

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

26.801

Isoelectric point:

6.942

Motif(s):

Type	Positions	Sequence
	203 -> 203	R
	208 -> 208	R
	11 -> 159	HRSIRHFTDEPISEAQREAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGW
	167 -> 169	KPR
	128 -> 131	YIGG

Classification:

Multifun Terms (GenProtEC)
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0016491 - oxidoreductase activity GO:0016657 - oxidoreductase activity, acting on NAD(P)H, nitrogenous group as acceptor GO:0010181 - FMN binding GO:0042803 - protein homodimerization activity GO:0034567 - chromate reductase activity
	biological_process	GO:0055114 - oxidation-reduction process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Merkley ED., et al., 2010
[2] Valle A., et al., 2012

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	MarA, Rob, SoxS
Repressed by:	OxyR

Reference(s)
[1] Merkley ED., Parson WW., Daggett V., 2010, Temperature dependence of the flexibility of thermophilic and mesophilic flavoenzymes of the nitroreductase fold., Protein Eng Des Sel 23(5):327-36
[2] Valle A., Le Borgne S., Bolivar J., Cabrera G., Cantero D., 2012, Study of the role played by NfsA, NfsB nitroreductase and NemA flavin reductase from Escherichia coli in the conversion of ethyl 2-(2'-nitrophenoxy)acetate to 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), a benzohydroxamic acid with interesting biological properties., Appl Microbiol Biotechnol 94(1):163-71