RegulonDB RegulonDB 10.7: Gene Form
   

nfsA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rimK nfsA ybjC grxA SoxS Rob MarA OxyR SoxS OxyR OxyR rimKp rimKp ybjCp ybjCp grxAp grxAp

Gene      
Name: nfsA    Texpresso search in the literature
Synonym(s): ECK0842, EG11261, b0851, mda18, mdaA, ybjB
Genome position(nucleotides): 891184 --> 891906 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.56
External database links:  
ASAP:
ABE-0002900
ECHOBASE:
EB1241
OU-MICROARRAY:
b0851
PORTECO:
nfsA
REGULONDB:
b0851
STRING:
511145.b0851
M3D: nfsA
COLOMBOS: nfsA
PortEco: b0851


Product      
Name: NADPH-dependent nitroreductase NfsA
Synonym(s): MdaA, NfsA, YbjB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 26.801
Isoelectric point: 6.942
Motif(s):
 
Type Positions Sequence
203 -> 203 R
208 -> 208 R
11 -> 159 HRSIRHFTDEPISEAQREAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGW
167 -> 169 KPR
128 -> 131 YIGG

 

Classification:
Multifun Terms (GenProtEC)  
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016491 - oxidoreductase activity
GO:0016657 - oxidoreductase activity, acting on NAD(P)H, nitrogenous group as acceptor
GO:0010181 - FMN binding
GO:0042803 - protein homodimerization activity
GO:0034567 - chromate reductase activity
biological_process GO:0055114 - oxidation-reduction process
Note(s): Note(s): ...[more].
Reference(s): [1] Merkley ED., et al., 2010
[2] Valle A., et al., 2012
External database links:  
DIP:
DIP-10334N
ECOCYC:
EG11261-MONOMER
ECOLIWIKI:
b0851
INTERPRO:
IPR000415
INTERPRO:
IPR016446
INTERPRO:
IPR029479
MODBASE:
P17117
PANTHER:
PTHR43425
PDB:
1F5V
PFAM:
PF00881
PRIDE:
P17117
PRODB:
PRO_000023377
PROTEINMODELPORTAL:
P17117
REFSEQ:
NP_415372
SMR:
P17117
UNIPROT:
P17117


Operon      
Name: ybjC-nfsA-rimK-ybjN         
Operon arrangement:
Transcription unit        Promoter
ybjC-nfsA-rimK-ybjN
rimK-ybjN
ybjN


Transcriptional Regulation      
Display Regulation             
Activated by: MarA, Rob, SoxS
Repressed by: OxyR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Merkley ED., Parson WW., Daggett V., 2010, Temperature dependence of the flexibility of thermophilic and mesophilic flavoenzymes of the nitroreductase fold., Protein Eng Des Sel 23(5):327-36

 [2] Valle A., Le Borgne S., Bolivar J., Cabrera G., Cantero D., 2012, Study of the role played by NfsA, NfsB nitroreductase and NemA flavin reductase from Escherichia coli in the conversion of ethyl 2-(2'-nitrophenoxy)acetate to 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), a benzohydroxamic acid with interesting biological properties., Appl Microbiol Biotechnol 94(1):163-71


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