RegulonDB RegulonDB 10.8: Gene Form
   

trpL gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

trpE yciV trpL yciO TrpR TrpR TrpR Cis-reg; leader anti-anti-terminator anti-terminator terminator terminator TSS_1702 TSS_1702 yciVp yciVp trpLp trpLp TSS_1700 TSS_1700 TSS_1699 TSS_1699 TSS_1698 TSS_1698 TSS_1697 TSS_1697 TSS_1696 TSS_1696 TSS_1695 TSS_1695

Gene      
Name: trpL    Texpresso search in the literature
Synonym(s): ECK1259, EG11274, b1265, trpEE
Genome position(nucleotides): 1323038 <-- 1323082 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 44.44
External database links:  
ASAP:
 ABE-0004246
ECHOBASE:
 EB1252
OU-MICROARRAY:
 b1265
PortEco:
 trpL
COLOMBOS: trpL


Product      
Name: trp operon leader peptide
Synonym(s): TrpL
Sequence: Get amino acid sequence Fasta Format
Molecular weight: 1.723
Isoelectric point: 11.828
Motif(s):
 
Type Positions Sequence
1 -> 14 MKAIFVLKGWWRTS

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.15 - tryptophan
Gene Ontology Terms (GO)  
biological_process GO:0000162 - tryptophan biosynthetic process
GO:0008652 - cellular amino acid biosynthetic process
GO:0009073 - aromatic amino acid family biosynthetic process
GO:0090357 - regulation of tryptophan metabolic process
Note(s): Note(s): ...[more].
Evidence: [IDA] Inferred from direct assay
Reference(s): [1] Dallaire P., et al., 2016
[2] Yanofsky C. 2000
[3] Yanofsky C., et al., 1981
External database links:  
ECOCYC:
 EG11274-MONOMER
ECOLIWIKI:
 b1265
INTERPRO:
 IPR013205
PFAM:
 PF08255
PRIDE:
 P0AD92
PRODB:
 PRO_000024123
REFSEQ:
 NP_415781
UNIPROT:
 P0AD92


Operon      
Name: trpLEDCBA         
Operon arrangement:
Transcription unit        Promoter
trpCBA
trpL
trpLEDCBA


Transcriptional Regulation      
Display Regulation             
Repressed by: TrpR


RNA cis-regulatory element    
   
Cis-reg; leader Tryptophan operon leader
   
   


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1695 1321481 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_1696 1321882 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_1697 1321905 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_1698 1322038 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_1699 1322047 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_1700 1322139 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_1702 1323345 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Dallaire P., Major F., 2016, Exploring Alternative RNA Structure Sets Using MC-Flashfold and db2cm., Methods Mol Biol 1490:237-51

 [2] Yanofsky C., 2000, Transcription attenuation: once viewed as a novel regulatory strategy., J Bacteriol 182(1):1-8

 [3] Yanofsky C., Platt T., Crawford IP., Nichols BP., Christie GE., Horowitz H., VanCleemput M., Wu AM., 1981, The complete nucleotide sequence of the tryptophan operon of Escherichia coli., Nucleic Acids Res 9(24):6647-68

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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