RegulonDB

Gene
Name:	thrL
Synonym(s):	ECK0001, EG11277, b0001
Genome position(nucleotides):	190 --> 255 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.52
External database links:

ASAP:	ABE-0000006
ECHOBASE:	EB1255
OU-MICROARRAY:	b0001
PORTECO:	thrL
REGULONDB:	b0001
STRING:	511145.b0001
M3D:	thrL
COLOMBOS:	thrL
PortEco:	b0001

Product

Name:

thr operon leader peptide

Synonym(s):

ThrL

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

2.138

Isoelectric point:

11.652

Motif(s):

Type	Positions	Sequence
	1 -> 21	MKRISTTITTTITITTGNGAG

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.8 - threonine
		3 - regulation
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	biological_process	GO:0008652 - cellular amino acid biosynthetic process GO:0009088 - threonine biosynthetic process GO:0031554 - regulation of DNA-templated transcription, termination GO:0031555 - transcriptional attenuation GO:0031556 - transcriptional attenuation by ribosome

Note(s):

Note(s): ...[more].

Evidence:

[IDA] Inferred from direct assay
[IMP] Inferred from mutant phenotype

Reference(s):

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

thrLABC

Operon arrangement:

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_1	38	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_3 (cluster)	683	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 683 to 684, where the highest frequency is at position 683. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[4]
promoter	TSS_4	688	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_5	703	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_6	1009	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_7	1176	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_8	1886	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_9	1965	forward	nd	[RS-EPT-CBR]	[4]
promoter	TSS_10	2581	forward	nd	[RS-EPT-CBR]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Johnson EJ., Cohen GN., Saint-Girons I., 1977, Threonyl-transfer ribonucleic acid synthetase and the regulation of the threonine operon in Escherichia coli., J Bacteriol 129(1):66-70
[2] Lynn SP., Burton WS., Donohue TJ., Gould RM., Gumport RI., Gardner JF., 1987, Specificity of the attenuation response of the threonine operon of Escherichia coli is determined by the threonine and isoleucine codons in the leader transcript., J Mol Biol 194(1):59-69
[3] Lynn SP., Gardner JF., Reznikoff WS., 1982, Attenuation regulation in the thr operon of Escherichia coli K-12: molecular cloning and transcription of the controlling region., J Bacteriol 152(1):363-71
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.