RegulonDB RegulonDB 10.7: Gene Form
   

thrL gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

thrA yjtD thrL DksA-ppGpp DksA-ppGpp Cis-reg; leader terminator anti-terminator anti-anti-terminator TSS_10 TSS_10 TSS_9 TSS_9 TSS_8 TSS_8 TSS_7 TSS_7 TSS_6 TSS_6 TSS_5 TSS_5 TSS_4 TSS_4 TSS_3 (cluster) TSS_3 (cluster) thrLp thrLp TSS_1 TSS_1

Gene      
Name: thrL    Texpresso search in the literature
Synonym(s): ECK0001, EG11277, b0001
Genome position(nucleotides): 190 --> 255 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.52
External database links:  
ASAP:
ABE-0000006
ECHOBASE:
EB1255
OU-MICROARRAY:
b0001
PORTECO:
thrL
REGULONDB:
b0001
STRING:
511145.b0001
M3D: thrL
COLOMBOS: thrL
PortEco: b0001


Product      
Name: thr operon leader peptide
Synonym(s): ThrL
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 2.138
Isoelectric point: 11.652
Motif(s):
 
Type Positions Sequence
1 -> 21 MKRISTTITTTITITTGNGAG

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.8 - threonine
  3 - regulation
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009088 - threonine biosynthetic process
GO:0031554 - regulation of DNA-templated transcription, termination
GO:0031555 - transcriptional attenuation
GO:0031556 - transcriptional attenuation by ribosome
Note(s): Note(s): ...[more].
Evidence: [IDA] Inferred from direct assay
[IMP] Inferred from mutant phenotype
Reference(s): [1] Johnson EJ., et al., 1977
[2] Lynn SP., et al., 1987
[3] Lynn SP., et al., 1982
External database links:  
ECOCYC:
EG11277-MONOMER
ECOLIWIKI:
b0001
INTERPRO:
IPR011720
PFAM:
PF08254
PRIDE:
P0AD86
REFSEQ:
NP_414542
UNIPROT:
P0AD86


Operon      
Name: thrLABC         
Operon arrangement:
Transcription unit        Promoter
thrL
thrLABC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1 38 forward nd [RS-EPT-CBR] [4]
  promoter TSS_3 (cluster) 683 forward For this promoter, there
Read more >
[RS-EPT-CBR] [4]
  promoter TSS_4 688 forward nd [RS-EPT-CBR] [4]
  promoter TSS_5 703 forward nd [RS-EPT-CBR] [4]
  promoter TSS_6 1009 forward nd [RS-EPT-CBR] [4]
  promoter TSS_7 1176 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_8 1886 forward nd [RS-EPT-CBR] [4]
  promoter TSS_9 1965 forward nd [RS-EPT-CBR] [4]
  promoter TSS_10 2581 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Johnson EJ., Cohen GN., Saint-Girons I., 1977, Threonyl-transfer ribonucleic acid synthetase and the regulation of the threonine operon in Escherichia coli., J Bacteriol 129(1):66-70

 [2] Lynn SP., Burton WS., Donohue TJ., Gould RM., Gumport RI., Gardner JF., 1987, Specificity of the attenuation response of the threonine operon of Escherichia coli is determined by the threonine and isoleucine codons in the leader transcript., J Mol Biol 194(1):59-69

 [3] Lynn SP., Gardner JF., Reznikoff WS., 1982, Attenuation regulation in the thr operon of Escherichia coli K-12: molecular cloning and transcription of the controlling region., J Bacteriol 152(1):363-71

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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