RegulonDB RegulonDB 10.8: Gene Form
   

hyfB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hyfB hyfC hyfA bcp FNR FhlA HyfR CRP hyfAp hyfAp

Gene      
Name: hyfB    Texpresso search in the literature
Synonym(s): ECK2478, EG11282, b2482, yffF
Genome position(nucleotides): 2601818 --> 2603836 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.73
Reference(s): [1] Andrews SC., et al., 1997
External database links:  
ASAP:
 ABE-0008181
ECHOBASE:
 EB1259
OU-MICROARRAY:
 b2482
PortEco:
 hyfB
STRING:
 511145.b2482
COLOMBOS: hyfB


Product      
Name: hydrogenase 4 component B
Synonym(s): HyfB, YffF
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 72.583
Isoelectric point: 9.063
Motif(s):
 
Type Positions Sequence
6 -> 26 LLTWSLILYLFASLASLFLLG
428 -> 448 AGPIAMVMLAVTGGLAVMCFV
80 -> 100 LSAFMVLVISLLVVVCSLYSL
200 -> 220 LSLSPGLASAVFLLAFFGFGA
273 -> 293 LWWGILVMAIGAISALLGVLY

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0044569 - [Ni-Fe] hydrogenase complex
molecular_function GO:0005515 - protein binding
GO:0016491 - oxidoreductase activity
GO:0008137 - NADH dehydrogenase (ubiquinone) activity
biological_process GO:0042773 - ATP synthesis coupled electron transport
GO:0055114 - oxidation-reduction process
Note(s): Note(s): ...[more].
External database links:  
DIP:
 DIP-9984N
ECOCYC:
 MONOMER0-153
ECOLIWIKI:
 b2482
INTERPRO:
 IPR001750
INTERPRO:
 IPR003918
MINT:
 MINT-1254904
PFAM:
 PF00361
PRIDE:
 P23482
PRINTS:
 PR01437
PRODB:
 PRO_000022964
REFSEQ:
 NP_416977
SMR:
 P23482
UNIPROT:
 P23482


Operon      
Name: hyfABCDEFGHIJR-focB         
Operon arrangement:
Transcription unit        Promoter
hyfABCDEFGHIJR-focB


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, FhlA, HyfR
Repressed by: FNR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Andrews SC., Berks BC., McClay J., Ambler A., Quail MA., Golby P., Guest JR., 1997, A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system., Microbiology 143 ( Pt 11):3633-47

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