RegulonDB RegulonDB 10.8: Gene Form
   

codA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

prpE codA codB cynR PurR Nac codBp codBp

Gene      
Name: codA    Texpresso search in the literature
Synonym(s): ECK0334, EG11326, b0337
Genome position(nucleotides): 356171 --> 357454 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.43
External database links:  
ASAP:
ABE-0001161
CGSC:
913
ECHOBASE:
EB1302
OU-MICROARRAY:
b0337
PortEco:
codA
STRING:
511145.b0337
COLOMBOS: codA


Product      
Name: cytosine/isoguanine deaminase
Synonym(s): CodA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 47.591
Isoelectric point: 6.097
Motif(s):
 
Type Positions Sequence
2 -> 427 SNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR
48 -> 405 DAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKVI
218 -> 218 E
13 -> 13 R
247 -> 247 H

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.33 - nucleotide and nucleoside conversions
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0102480 - 5-fluorocytosine deaminase activity
GO:0016787 - hydrolase activity
GO:0046872 - metal ion binding
GO:0016810 - hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
GO:0016814 - hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines
GO:0008270 - zinc ion binding
GO:0004131 - cytosine deaminase activity
GO:0008198 - ferrous iron binding
GO:0042802 - identical protein binding
GO:0035888 - isoguanine deaminase activity
biological_process GO:0019858 - cytosine metabolic process
GO:0006209 - cytosine catabolic process
Reference(s): [1] Austin EA., et al., 1993
External database links:  
DIP:
DIP-9306N
ECOCYC:
CYTDEAM-MONOMER
ECOLIWIKI:
b0337
INTERPRO:
IPR032466
INTERPRO:
IPR013108
INTERPRO:
IPR011059
MINT:
MINT-1307853
MODBASE:
P25524
PDB:
1RA0
PDB:
1RA5
PDB:
1RAK
PDB:
3G77
PDB:
3O7U
PDB:
3R0D
PDB:
3RN6
PDB:
1R9Z
PDB:
1R9X
PDB:
1R9Y
PDB:
1K70
PDB:
1K6W
PFAM:
PF07969
PRIDE:
P25524
PRODB:
PRO_000022316
REFSEQ:
NP_414871
SMR:
P25524
UNIPROT:
P25524


Operon      
Name: codBA         
Operon arrangement:
Transcription unit        Promoter
codBA


Transcriptional Regulation      
Display Regulation             
Activated by: Nac
Repressed by: PurR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Austin EA., Huber BE., 1993, A first step in the development of gene therapy for colorectal carcinoma: cloning, sequencing, and expression of Escherichia coli cytosine deaminase., Mol Pharmacol 43(3):380-7


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