RegulonDB RegulonDB 10.8: Gene Form
   

mnmA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mnmA hflD nudJ TSS_1478 TSS_1478 TSS_1477 TSS_1477 TSS_1476 TSS_1476 hflDp hflDp TSS_1475 TSS_1475

Gene      
Name: mnmA    Texpresso search in the literature
Synonym(s): ECK1119, EG11344, asuE, b1133, trmU, ycfB
Genome position(nucleotides): 1192667 <-- 1193773 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.75
External database links:  
ASAP:
 ABE-0003815
CGSC:
 37613
ECHOBASE:
 EB1320
MIM:
 580000
MIM:
 613070
OU-MICROARRAY:
 b1133
PortEco:
 mnmA
STRING:
 511145.b1133
COLOMBOS: mnmA


Product      
Name: tRNA-specific 2-thiouridylase
Synonym(s): AsuE, MnmA, TrmU, YcfB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 40.959
Isoelectric point: 4.685
Motif(s):
 
Type Positions Sequence
200 -> 200 F
37 -> 37 M
102 -> 102 C
151 -> 151 Q
128 -> 128 H

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0003723 - RNA binding
GO:0016783 - sulfurtransferase activity
GO:0000049 - tRNA binding
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
biological_process GO:0006400 - tRNA modification
GO:0008033 - tRNA processing
GO:0002143 - tRNA wobble position uridine thiolation
Note(s): Note(s): ...[more].
External database links:  
DIP:
 DIP-11035N
ECOCYC:
 EG11344-MONOMER
ECOLIWIKI:
 b1133
INTERPRO:
 IPR023382
INTERPRO:
 IPR014729
INTERPRO:
 IPR004506
MODBASE:
 P25745
PANTHER:
 PTHR11933
PDB:
 2DET
PDB:
 2DER
PDB:
 2DEU
PFAM:
 PF03054
PRIDE:
 P25745
PRODB:
 PRO_000023254
REFSEQ:
 NP_415651
SMR:
 P25745
UNIPROT:
 P25745


Operon      
Name: mnmA         
Operon arrangement:
Transcription unit        Promoter
mnmA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1475 1192505 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1476 1192966 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1477 1193560 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_1478 1193801 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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