RegulonDB RegulonDB 10.7: Gene Form
   

dadA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ycgB dadA dadX Lrp Lrp Lrp CRP CRP Lrp CRP Lrp Lrp Lrp Lrp Lrp Lrp Lrp terminator dadAp3 dadAp3 dadAp2 dadAp2 dadAp1 dadAp1 ycgBp ycgBp TSS_1516 TSS_1516 TSS_1515 TSS_1515 TSS_1514 TSS_1514 TSS_1513 TSS_1513

Gene      
Name: dadA    Texpresso search in the literature
Synonym(s): ECK1177, EG11407, b1189, dadR
Genome position(nucleotides): 1237571 --> 1238869 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
56.27
External database links:  
ASAP:
ABE-0003995
CGSC:
884
CGSC:
18433
ECHOBASE:
EB1379
OU-MICROARRAY:
b1189
PORTECO:
dadA
REGULONDB:
b1189
STRING:
511145.b1189
M3D: dadA
COLOMBOS: dadA
PortEco: b1189


Product      
Name: D-amino acid dehydrogenase
Synonym(s): DadA, DadR
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,inner membrane
Molecular weight: 47.607
Isoelectric point: 6.605
Motif(s):
 
Type Positions Sequence
2 -> 397 RVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDAGVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDL
3 -> 17 VVILGSGVVGVASAW

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.3 - amino acids
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0016020 - membrane
GO:0005886 - plasma membrane
molecular_function GO:0016491 - oxidoreductase activity
GO:0008718 - D-amino-acid dehydrogenase activity
biological_process GO:0019478 - D-amino acid catabolic process
GO:0055114 - oxidation-reduction process
GO:0019480 - L-alanine oxidation to pyruvate via D-alanine
GO:0055130 - D-alanine catabolic process
Reference(s): [1] Franklin FC., et al., 1981
External database links:  
DIP:
DIP-6852N
ECOCYC:
DALADEHYDROGA-MONOMER
ECOLIWIKI:
b1189
INTERPRO:
IPR006076
INTERPRO:
IPR023080
INTERPRO:
IPR036188
MODBASE:
P0A6J5
PFAM:
PF01266
PRIDE:
P0A6J5
PRODB:
PRO_000022396
PROTEINMODELPORTAL:
P0A6J5
REFSEQ:
NP_415707
SMR:
P0A6J5
UNIPROT:
P0A6J5


Operon      
Name: dadAX         
Operon arrangement:
Transcription unit        Promoter
dadAX
dadAX
dadAX


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: CRP, Lrp


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1513 1236798 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1514 1236840 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1515 1237036 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_1516 1237229 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Franklin FC., Venables WA., Wijsman HJ., 1981, Genetic studies of D-alanine-dehydrogenase-less mutants of Escherichia coli K12., Genet Res 38(2):197-208

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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