RegulonDB RegulonDB 10.8: Gene Form
   

nrdD gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nrdD treC nrdG FNR FNR NrdR NrdR nrdDp nrdDp

Gene      
Name: nrdD    Texpresso search in the literature
Synonym(s): ECK4233, EG11417, b4238
Genome position(nucleotides): 4460522 <-- 4462660 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.53
External database links:  
ASAP:
ABE-0013865
CGSC:
34589
ECHOBASE:
EB1388
OU-MICROARRAY:
b4238
PortEco:
nrdD
STRING:
511145.b4238
COLOMBOS: nrdD


Product      
Name: anaerobic ribonucleoside-triphosphate reductase
Synonym(s): NrdD
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 80.023
Isoelectric point: 6.81
Motif(s):
 
Type Positions Sequence
647 -> 647 C
682 -> 682 Y
420 -> 420 A
644 -> 644 C
662 -> 662 C

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.15 - 2'-deoxyribonucleotide metabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0031250 - anaerobic ribonucleoside-triphosphate reductase complex
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016491 - oxidoreductase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0008270 - zinc ion binding
GO:0008998 - ribonucleoside-triphosphate reductase activity
GO:0042803 - protein homodimerization activity
GO:0051065 - CTP reductase activity
GO:0032556 - pyrimidine deoxyribonucleotide binding
GO:0032564 - dATP binding
GO:0032567 - dGTP binding
biological_process GO:0006260 - DNA replication
GO:0009265 - 2'-deoxyribonucleotide biosynthetic process
GO:0015949 - nucleobase-containing small molecule interconversion
GO:0055114 - oxidation-reduction process
Note(s): Note(s): ...[more].
Reference(s): [1] Eliasson R., et al., 1992
[2] Harder J., et al., 1992
[3] Ollagnier S., et al., 1996
[4] Ollagnier S., et al., 1997
[5] Pontis E., et al., 1991
[6] Tamarit J., et al., 1999
[7] Wei Y., et al., 2014
External database links:  
DIP:
DIP-10358N
ECOCYC:
RIBONUCLEOSIDE-TRIP-REDUCT-MONOMER
ECOLIWIKI:
b4238
INTERPRO:
IPR019777
INTERPRO:
IPR012833
INTERPRO:
IPR005144
INTERPRO:
IPR001150
MODBASE:
P28903
PFAM:
PF03477
PFAM:
PF13597
PRIDE:
P28903
PROSITE:
PS51161
PROSITE:
PS51149
PROSITE:
PS00850
REFSEQ:
NP_418659
SMR:
P28903
SWISSMODEL:
P28903
UNIPROT:
P28903


Operon      
Name: nrdDG         
Operon arrangement:
Transcription unit        Promoter
nrdDG
nrdG


Transcriptional Regulation      
Display Regulation             
Activated by: FNR
Repressed by: H-NS, NrdR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Eliasson R., Pontis E., Fontecave M., Gerez C., Harder J., Jornvall H., Krook M., Reichard P., 1992, Characterization of components of the anaerobic ribonucleotide reductase system from Escherichia coli., J Biol Chem 267(35):25541-7

 [2] Harder J., Eliasson R., Pontis E., Ballinger MD., Reichard P., 1992, Activation of the anaerobic ribonucleotide reductase from Escherichia coli by S-adenosylmethionine., J Biol Chem 267(35):25548-52

 [3] Ollagnier S., Mulliez E., Gaillard J., Eliasson R., Fontecave M., Reichard P., 1996, The anaerobic Escherichia coli ribonucleotide reductase. Subunit structure and iron sulfur center., J Biol Chem 271(16):9410-6

 [4] Ollagnier S., Mulliez E., Schmidt PP., Eliasson R., Gaillard J., Deronzier C., Bergman T., Graslund A., Reichard P., Fontecave M., 1997, Activation of the anaerobic ribonucleotide reductase from Escherichia coli. The essential role of the iron-sulfur center for S-adenosylmethionine reduction., J Biol Chem 272(39):24216-23

 [5] Pontis E., Sun XY., Jornvall H., Krook M., Reichard P., 1991, ClpB proteins copurify with the anaerobic Escherichia coli reductase., Biochem Biophys Res Commun 180(3):1222-6

 [6] Tamarit J., Mulliez E., Meier C., Trautwein A., Fontecave M., 1999, The anaerobic ribonucleotide reductase from Escherichia coli. The small protein is an activating enzyme containing a [4fe-4s](2+) center., J Biol Chem 274(44):31291-6

 [7] Wei Y., Mathies G., Yokoyama K., Chen J., Griffin RG., Stubbe J., 2014, A chemically competent thiosulfuranyl radical on the Escherichia coli class III ribonucleotide reductase., J Am Chem Soc 136(25):9001-13


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