RegulonDB RegulonDB 8.6: Gene Form
   

gadB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pqqL gadC gadB GadX GadW GadX GadW GadX GadW Fis GadX GadW RcsB CRP GadE Fis GadX Fis GadX Fis terminator TSS_1670 TSS_1670 gadBp gadBp TSS_1669 TSS_1669 gadCp1 gadCp1 gadCp6 gadCp6 gadCp5 gadCp5

Gene      
Name: gadB    Texpresso search in the literature
Synonym(s): b1493
Genome position(nucleotides): 1568669 <-- 1570069 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.32
External database links:  
ECOCYC:
EG11490
GENBANK:
1787769
GENBANK_RefSeq:
16129452
OU-MICROARRAY:
b1493
M3D: gadB
COLOMBOS: gadB
PortEco: b1493


Product      
Name: glutamate decarboxylase B subunit
Synonym(s): GadB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,membrane
Molecular weight: 52.668
Isoelectric point: 5.14
Motif(s):
 
Type Positions Sequence
276 -> 276 K
126 -> 127 SS

 

Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.5 - adaptations --> 5.5.4 - pH
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0016831 - carboxy-lyase activity
GO:0004351 - glutamate decarboxylase activity
GO:0030170 - pyridoxal phosphate binding
biological_process GO:0019752 - carboxylic acid metabolic process
GO:0006536 - glutamate metabolic process
GO:0051454 - intracellular pH elevation
Note(s): Note(s): ...[more].
Reference(s): [1] De Biase D., et al., 1999
[2] Lin J., et al., 1995
[3] Smith DK., et al., 1992
External database links:  
ECOCYC:
GLUTDECARBOXB-MONOMER
EXPASY:
EC-4.1.1.15
GENPROTEC:
b1493
PDB:
2DGM
PDB:
3FZ6
PDB:
3FZ7
PDB:
3FZ8
PDB:
2DGK
PDB:
1PMO
PDB:
1PMM
PDB:
2DGL


Operon      
Name: gadBC         
Operon arrangement:
Transcription unit        Promoter
gadC
gadC
gadC
gadBC


Regulation by small RNA    
  Display Regulation
small RNA oxyS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1669 1569644 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_1670 1570097 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] De Biase D., Tramonti A., Bossa F., Visca P., 1999, The response to stationary-phase stress conditions in Escherichia coli: role and regulation of the glutamic acid decarboxylase system., Mol Microbiol. 32(6):1198-211

 [2] Lin J., Lee IS., Frey J., Slonczewski JL., Foster JW., 1995, Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli., J Bacteriol. 177(14):4097-104

 [3] Smith DK., Kassam T., Singh B., Elliott JF., 1992, Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci., J Bacteriol. 174(18):5820-6

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu¿¿iz-Rascado L, Garc¿¿a-Sotelo JS, Weiss V, Solano-Lira H, Mart¿¿nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern¿¿ndez S, Alquicira-Hern¿¿ndez K, L¿¿pez-Fuentes A, Porr¿¿n-Sotelo L, Huerta AM, Bonavides-Mart¿¿nez C, Balderas-Mart¿¿nez YI, Pannier L, Olvera M, Labastida A, Jim¿¿nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch¿¿vez V, Hern¿¿ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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