RegulonDB RegulonDB 10.8: Gene Form
   

metQ gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

metQ metI rcsF metN trmO anti-anti-terminator anti-terminator terminator TSS_412 TSS_412 TSS_411 (cluster) TSS_411 (cluster) TSS_410 TSS_410 TSS_409 TSS_409 TSS_408 (cluster) TSS_408 (cluster) TSS_407 TSS_407 TSS_406 TSS_406 TSS_405 TSS_405 TSS_404 TSS_404 TSS_403 TSS_403 TSS_402 TSS_402 TSS_401 TSS_401 TSS_400 TSS_400 TSS_399 TSS_399 TSS_398 TSS_398

Gene      
Name: metQ    Texpresso search in the literature
Synonym(s): ECK0197, EG11504, b0197, metD, yaeC
Genome position(nucleotides): 220113 <-- 220928 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.14
Reference(s): [1] Merlin C., et al., 2002
External database links:  
ASAP:
 ABE-0000666
ECHOBASE:
 EB1467
OU-MICROARRAY:
 b0197
PortEco:
 metQ
STRING:
 511145.b0197
COLOMBOS: metQ


Product      
Name: L-methionine/D-methionine ABC transporter membrane anchored binding protein
Synonym(s): MetD, MetQ, YaeC
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,periplasmic space
Molecular weight: 29.432
Isoelectric point: 4.889
Motif(s):
 
Type Positions Sequence
23 -> 271 CGQDEKDPNHIKVGVIVGAEQQVAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYKLVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDGVGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGIFVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW
33 -> 271 IKVGVIVGAEQQVAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYKLVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDGVGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGIFVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW
200 -> 200 V
1 -> 22 MAFKFKTFAAVGALIGSLALVG
229 -> 229 N

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.p - ABC superfamily, periplasmic binding component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0055052 - ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
GO:0046658 - anchored component of plasma membrane
molecular_function GO:0005515 - protein binding
biological_process GO:0006865 - amino acid transport
GO:0048473 - D-methionine transport
GO:0015821 - methionine transport
Note(s): Note(s): ...[more].
Reference(s): [2] Gonnet P., et al., 2004
External database links:  
DIP:
 DIP-11196N
ECOCYC:
 METQ-MONOMER
ECOLIWIKI:
 b0197
INTERPRO:
 IPR004872
MODBASE:
 P28635
PANTHER:
 PTHR30429
PDB:
 4YAH
PDB:
 6CVL
PFAM:
 PF03180
PRIDE:
 P28635
PRODB:
 PRO_000023219
PROSITE:
 PS51257
REFSEQ:
 NP_414739
SMR:
 P28635
UNIPROT:
 P28635


Operon      
Name: metNIQ         
Operon arrangement:
Transcription unit        Promoter
metNIQ


Transcriptional Regulation      
Display Regulation             
Activated by: HypT
Repressed by: MetJ


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_398 220022 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_399 220386 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_400 220394 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_401 220539 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_402 220543 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_403 220638 forward nd [RS-EPT-CBR] [3]
  promoter TSS_404 220964 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_405 220970 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_406 220975 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_407 221009 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_408 (cluster) 221015 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [3]
  promoter TSS_409 221017 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_410 221019 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_411 (cluster) 221041 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [3]
  promoter TSS_412 221053 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Merlin C., Gardiner G., Durand S., Masters M., 2002, The Escherichia coli metD locus encodes an ABC transporter which includes Abc (MetN), YaeE (MetI), and YaeC (MetQ)., J Bacteriol 184(19):5513-7

 [2] Gonnet P., Rudd KE., Lisacek F., 2004, Fine-tuning the prediction of sequences cleaved by signal peptidase II: a curated set of proven and predicted lipoproteins of Escherichia coli K-12., Proteomics 4(6):1597-613

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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