RegulonDB RegulonDB 11.1: Gene Form
   

fixA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

caiT fixB fixA CaiF CRP CaiF CaiF CRP CaiF ArcA ArcA FNR FNR CRP ArcA ArcA fixAp fixAp caiTp caiTp

Gene      
Name: fixA    Texpresso search in the literature
Synonym(s): ECK0042, EG11562, b0041, yaaQ
Genome position(nucleotides): 42403 --> 43173
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.53
Reference(s): [1] Eichler K., et al., 1994
External database links:  
ASAP:
 ABE-0000145
ECHOBASE:
 EB1523
ECOLIHUB:
 fixA
MIM:
 231680
OU-MICROARRAY:
 b0041
STRING:
 511145.b0041
COLOMBOS: fixA


Product      
Name: putative electron transfer flavoprotein FixA
Synonym(s): FixA, YaaQ, putative electron transfer flavoprotein subunit, ETFP adenine nucleotide-binding domain
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space
Molecular weight: 27.144
Isoelectric point: 4.215
Motif(s):
 
Type Positions Sequence Comment
28 -> 210 SKADAKISQYDLNAIEAACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTASALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVERELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAAD
216 -> 217 EA UniProt: In Ref. 1; CAA50797..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.4 - energy production/transport
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
molecular_function GO:0005515 - protein binding
GO:0009055 - electron transfer activity
biological_process GO:0009437 - carnitine metabolic process
GO:0042413 - carnitine catabolic process
GO:0022900 - electron transport chain
Note(s): Note(s): ...[more].
Evidence: [COMP-HINF-FN-FROM-SEQ] Human inference of function from sequence
[EXP-IMP] Inferred from mutant phenotype
Reference(s): [2] Eichler K., et al., 1995
[3] Walt A., et al., 2002
External database links:  
ALPHAFOLD:
 P60566
ECOCYC:
 EG11562-MONOMER
ECOLIWIKI:
 b0041
INTERPRO:
 IPR033948
INTERPRO:
 IPR023463
INTERPRO:
 IPR014730
INTERPRO:
 IPR014729
INTERPRO:
 IPR012255
INTERPRO:
 IPR000049
MODBASE:
 P60566
PANTHER:
 PTHR21294
PFAM:
 PF01012
PRIDE:
 P60566
PRODB:
 PRO_000022628
PROSITE:
 PS01065
REFSEQ:
 NP_414583
SMART:
 SM00893
SMR:
 P60566
UNIPROT:
 P60566


Operon      
Name: fixABCX         
Operon arrangement:
Transcription unit        Promoter
fixABCX


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, FNR, CaiF


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Eichler K., Bourgis F., Buchet A., Kleber HP., Mandrand-Berthelot MA., 1994, Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli., Mol Microbiol 13(5):775-86

 [2] Eichler K., Buchet A., Bourgis F., Kleber HP., Mandrand-Berthelot MA., 1995, The fix Escherichia coli region contains four genes related to carnitine metabolism., J Basic Microbiol 35(4):217-27

 [3] Walt A., Kahn ML., 2002, The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli., J Bacteriol 184(14):4044-7

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