RegulonDB

Gene
Name:	gcl
Synonym(s):	ECK0500, EG11583, b0507
Genome position(nucleotides):	533916 --> 535697 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.92
External database links:

ASAP:	ABE-0001754
CGSC:	31107
ECHOBASE:	EB1542
OU-MICROARRAY:	b0507
PortEco:	gcl
STRING:	511145.b0507
COLOMBOS:	gcl

Product

Name:

glyoxylate carboligase

Synonym(s):

Gcl

Sequence:

Get amino acid sequence Fasta Format

Molecular weight:

64.732

Isoelectric point:

5.982

Motif(s):

Type	Positions	Sequence
	393 -> 553	IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEV
	4 -> 172	MRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIE
	193 -> 329	IEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLL
	2 -> 593	AKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINADAAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAADAPTETCFMHYE

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.21 - glyoxylate degradation
		1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.25 - glycolate metabolism
Gene Ontology Terms (GO)
	molecular_function	GO:0003824 - catalytic activity GO:0016829 - lyase activity GO:0009028 - tartronate-semialdehyde synthase activity GO:0000287 - magnesium ion binding GO:0030976 - thiamine pyrophosphate binding GO:0042802 - identical protein binding GO:0071949 - FAD binding
	biological_process	GO:0009436 - glyoxylate catabolic process GO:0046296 - glycolate catabolic process

Note(s):

Note(s): ...[more].

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MINT:

PANTHER:

PDB:

PFAM:

PFAM:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

gcl-hyi-glxR-ybbW-allB-ybbY-glxK

Operon arrangement:

Transcription unit	Promoter
gcl-hyi-glxR-ybbVW-allB-ybbY-glxK	gclp
allB	allBp3
allB	allBp2
allB	allBp
ybbY-glxK	ybbYp
glxK	glxKp

Transcriptional Regulation

Display Regulation

Repressed by:

AllR

Growth Conditions:

[1]	C: Escherichia coli\| allS knockout mutant\| minimal medium\| D-xylose 120 µM; ammonium sulfate 20 mM\| mid exponential phase E: Escherichia coli\| allS knockout mutant\| minimal medium\| D-xylose 120 µM; ammonium sulfate 20 mM; glyoxylate\| mid exponential phase
[2]	C: Escherichia coli\| minimal medium\| D-xylose 120 µM; ammonium sulfate 20 mM\| mid exponential phase E: Escherichia coli\| minimal medium\| D-xylose 120 µM; ammonium sulfate 20 mM; glyoxylate\| mid exponential phase

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_671	533637	forward	nd	[RS-EPT-CBR]	[1]
promoter	TSS_672	534816	forward	nd	[RS-EPT-CBR]	[1]
promoter	TSS_673	534825	forward	nd	[RS-EPT-CBR]	[1]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.