RegulonDB RegulonDB 10.7: Gene Form
   

panB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

yadC panC panB panBp panBp TSS_272 TSS_272 panCp2 panCp2 panCp4 panCp4

Gene      
Name: panB    Texpresso search in the literature
Synonym(s): ECK0133, EG11675, b0134
Genome position(nucleotides): 148807 <-- 149601 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.59
External database links:  
ASAP:
ABE-0000467
CGSC:
427
ECHOBASE:
EB1626
OU-MICROARRAY:
b0134
PORTECO:
panB
REGULONDB:
b0134
STRING:
511145.b0134
M3D: panB
COLOMBOS: panB
PortEco: b0134


Product      
Name: 3-methyl-2-oxobutanoate hydroxymethyltransferase
Synonym(s): PanB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 28.237
Isoelectric point: 4.952
Motif(s):
 
Type Positions Sequence
7 -> 8 SL
124 -> 124 Q
4 -> 258 TTISLLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVGDSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAATVMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAGDQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDAFGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPG
15 -> 15 E
12 -> 12 Y

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.5 - Coenzyme A and its modification
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0003864 - 3-methyl-2-oxobutanoate hydroxymethyltransferase activity
GO:0000287 - magnesium ion binding
GO:0042802 - identical protein binding
biological_process GO:0015940 - pantothenate biosynthetic process
GO:0033317 - pantothenate biosynthetic process from valine
Note(s): Note(s): ...[more].
Reference(s): [1] Lobley CM., et al., 2003
[2] Merkel WK., et al., 1996
[3] Vallari DS., et al., 1985
External database links:  
DIP:
DIP-10436N
ECOCYC:
3-CH3-2-OXOBUTANOATE-OH-CH3-XFER-MONOMER
ECOLIWIKI:
b0134
INTERPRO:
IPR040442
INTERPRO:
IPR003700
INTERPRO:
IPR015813
MINT:
MINT-1249247
MODBASE:
P31057
PANTHER:
PTHR20881
PDB:
1M3U
PFAM:
PF02548
PRIDE:
P31057
PRODB:
PRO_000023488
PROTEINMODELPORTAL:
P31057
REFSEQ:
NP_414676
SMR:
P31057
UNIPROT:
P31057


Operon      
Name: panBC         
Operon arrangement:
Transcription unit        Promoter
panBC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter panCp4 148910 reverse Similarity to the consensus
Read more >
[ICWHO] [4]
  promoter panCp2 148920 reverse Similarity to the consensus
Read more >
[ICWHO] [4]
  promoter TSS_272 149617 reverse nd [RS-EPT-CBR] [5]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Lobley CM., Schmitzberger F., Kilkenny ML., Whitney H., Ottenhof HH., Chakauya E., Webb ME., Birch LM., Tuck KL., Abell C., Smith AG., Blundell TL., 2003, Structural insights into the evolution of the pantothenate-biosynthesis pathway., Biochem Soc Trans 31(Pt 3):563-71

 [2] Merkel WK., Nichols BP., 1996, Characterization and sequence of the Escherichia coli panBCD gene cluster., FEMS Microbiol Lett 143(2-3):247-52

 [3] Vallari DS., Rock CO., 1985, Isolation and characterization of Escherichia coli pantothenate permease (panF) mutants., J Bacteriol 164(1):136-42

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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