RegulonDB RegulonDB 10.7: Gene Form
   

pnuC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nadA zitB pnuC ybgS zitBp2 zitBp2 zitBp1 zitBp1 TSS_913 TSS_913 TSS_912 TSS_912 TSS_911 TSS_911 TSS_910 TSS_910 TSS_909 TSS_909 TSS_908 TSS_908 TSS_907 TSS_907 TSS_906 (cluster) TSS_906 (cluster) TSS_905 TSS_905 TSS_904 (cluster) TSS_904 (cluster) TSS_903 (cluster) TSS_903 (cluster) TSS_902 TSS_902

Gene      
Name: pnuC    Texpresso search in the literature
Synonym(s): ECK0740, EG11700, b0751
Genome position(nucleotides): 783166 --> 783885 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
48.19
External database links:  
ASAP:
ABE-0002547
ECHOBASE:
EB1651
OU-MICROARRAY:
b0751
PORTECO:
pnuC
REGULONDB:
b0751
STRING:
511145.b0751
M3D: pnuC
COLOMBOS: pnuC
PortEco: b0751


Product      
Name: nicotinamide riboside transporter
Synonym(s): PnuC
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 26.996
Isoelectric point: 7.337
Motif(s):
 
Type Positions Sequence
185 -> 192 WLLWVIIN
36 -> 36 C
11 -> 11 V
110 -> 127 LSWLAVCVVSIGLMTVFI
158 -> 177 AFPFWDSCMMVLSIVAMILM

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.33 - nucleotide and nucleoside conversions
  4 - transport --> 4.9.A - Transporters of Unknown Classification
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0034257 - nicotinamide riboside transmembrane transporter activity
biological_process GO:0034258 - nicotinamide riboside transport
Note(s): Note(s): ...[more].
Reference(s): [1] Bali AP., et al., 2018
External database links:  
ECOCYC:
PNUC-MONOMER
ECOLIWIKI:
b0751
INTERPRO:
IPR006419
PANTHER:
PTHR36122
PFAM:
PF04973
PRIDE:
P0AFK2
PRODB:
PRO_000023564
PROTEINMODELPORTAL:
P0AFK2
REFSEQ:
NP_415272
SMR:
P0AFK2
UNIPROT:
P0AFK2


Operon      
Name: nadA-pnuC         
Operon arrangement:
Transcription unit        Promoter
 


Transcriptional Regulation      
Display Regulation             
Repressed by: NadR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_902 782098 forward nd [RS-EPT-CBR] [2]
  promoter TSS_903 (cluster) 782352 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_904 (cluster) 782372 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_905 782376 forward nd [RS-EPT-CBR] [2]
  promoter TSS_906 (cluster) 782394 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_907 782424 forward nd [RS-EPT-CBR] [2]
  promoter TSS_908 783540 forward nd [RS-EPT-CBR] [2]
  promoter TSS_909 783611 forward nd [RS-EPT-CBR] [2]
  promoter TSS_910 783630 forward nd [RS-EPT-CBR] [2]
  promoter TSS_911 784191 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_912 784253 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_913 784403 reverse nd [RS-EPT-CBR] [2]
  promoter zitBp1 784848 reverse Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter zitBp2 784858 reverse Similarity to the consensus
Read more >
[ICWHO] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Bali AP., Genee HJ., Sommer MOA., 2018, Directed Evolution of Membrane Transport Using Synthetic Selections., ACS Synth Biol 7(3):789-793

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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