RegulonDB RegulonDB 10.8: Gene Form
   

gmhB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rrsH metN gmhB metI Fis Fis Fis Fis MetJ MetJ DksA DksA-ppGpp DksA-ppGpp ppGpp TSS_419 TSS_419 TSS_418 TSS_418 rrsHp2 rrsHp2 rrsHp1 rrsHp1 gmhBp gmhBp metNp metNp TSS_415 TSS_415 TSS_414 TSS_414 TSS_413 (cluster) TSS_413 (cluster)

Gene      
Name: gmhB    Texpresso search in the literature
Synonym(s): ECK0200, EG11736, b0200, yaeD
Genome position(nucleotides): 222833 --> 223408 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.17
External database links:  
ASAP:
 ABE-0000675
ECHOBASE:
 EB1687
MIM:
 609560
MIM:
 617069
OU-MICROARRAY:
 b0200
PortEco:
 gmhB
STRING:
 511145.b0200
COLOMBOS: gmhB


Product      
Name: D-glycero-β-D-manno-heptose-1,7-bisphosphate 7-phosphatase
Synonym(s): D,D-heptose 1,7-bisphosphate phosphatase, GmhB, YaeD
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 21.294
Isoelectric point: 4.774
Motif(s):
 
Type Positions Sequence
92 -> 92 C
107 -> 107 C
111 -> 111 K
110 -> 111 RK
137 -> 137 K

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.2 - core region
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016787 - hydrolase activity
GO:0046872 - metal ion binding
GO:0016791 - phosphatase activity
GO:0008270 - zinc ion binding
GO:0000287 - magnesium ion binding
GO:0034200 - D,D-heptose 1,7-bisphosphate phosphatase activity
biological_process GO:0005975 - carbohydrate metabolic process
GO:0009103 - lipopolysaccharide biosynthetic process
GO:0009244 - lipopolysaccharide core region biosynthetic process
GO:0016311 - dephosphorylation
GO:0097171 - ADP-L-glycero-beta-D-manno-heptose biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Li T., et al., 2014
External database links:  
DIP:
 DIP-47998N
ECOCYC:
 EG11736-MONOMER
ECOLIWIKI:
 b0200
INTERPRO:
 IPR036412
INTERPRO:
 IPR023214
INTERPRO:
 IPR006549
INTERPRO:
 IPR006543
INTERPRO:
 IPR004446
MODBASE:
 P63228
PANTHER:
 PTHR42891
PDB:
 3L8E
PDB:
 3L8F
PDB:
 3L8G
PDB:
 3ESR
PDB:
 2GMW
PDB:
 3ESQ
PDB:
 3L1V
PDB:
 3L1U
PFAM:
 PF13242
PRIDE:
 P63228
PRODB:
 PRO_000022821
REFSEQ:
 NP_414742
SMR:
 P63228
UNIPROT:
 P63228


Operon      
Name: gmhB         
Operon arrangement:
Transcription unit        Promoter
gmhB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_413 (cluster) 221681 reverse For this promoter, there
Read more > 		[RS-EPT-CBR] [2]
  promoter TSS_414 221903 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_415 221928 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_418 223594 forward nd [RS-EPT-CBR] [2]
  promoter TSS_419 223656 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Li T., Wen L., Williams A., Wu B., Li L., Qu J., Meisner J., Xiao Z., Fang J., Wang PG., 2014, Chemoenzymatic synthesis of ADP-d-glycero-β-d-manno-heptose and study of the substrate specificity of HldE., Bioorg Med Chem 22(3):1139-47

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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