RegulonDB RegulonDB 10.8: Gene Form
   

otsA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

otsA araH otsB uspC otsBp otsBp TSS_2235 TSS_2235 otsBp2 otsBp2 otsAp otsAp

Gene      
Name: otsA    Texpresso search in the literature
Synonym(s): ECK1895, EG11751, b1896
Genome position(nucleotides): 1980188 <-- 1981612 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
49.19
External database links:  
ASAP:
ABE-0006318
CGSC:
18073
ECHOBASE:
EB1701
OU-MICROARRAY:
b1896
PortEco:
otsA
STRING:
511145.b1896
COLOMBOS: otsA


Product      
Name: trehalose-6-phosphate synthase
Synonym(s): OtsA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 53.611
Isoelectric point: 6.854
Motif(s):
 
Type Positions Sequence
2 -> 474 SRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSGETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPAWDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAESQQRDKVATFPKLA
366 -> 370 LVAKE
17 -> 452 HAASAGGLAVGILGALKAAGGLWFGWSGETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPAWDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLK
22 -> 23 GG

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.9 - misc. glucose metabolism
  5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
  5 - cell processes --> 5.5 - adaptations --> 5.5.2 - temperature extremes
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016757 - transferase activity, transferring glycosyl groups
GO:0016740 - transferase activity
GO:0003825 - alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity
biological_process GO:0006950 - response to stress
GO:0006970 - response to osmotic stress
GO:0006974 - cellular response to DNA damage stimulus
GO:0005992 - trehalose biosynthetic process
GO:0070415 - trehalose metabolism in response to cold stress
Note(s): Note(s): ...[more].
Reference(s): [1] Guo W., et al., 2012
[2] Joseph TC., et al., 2010
[3] Kong X., et al., 2001
[4] Li H., et al., 2012
[5] Nguyen Ado Q., et al., 2013
[6] Peng S., et al., 2014
[7] Schultz T., et al., 2007
[8] Stoebel DM., et al., 2010
[9] Tondervik A., et al., 2006
[10] Worden CR., et al., 2009
External database links:  
CAZY:
GT20
DIP:
DIP-10417N
ECOCYC:
TREHALOSE6PSYN-MONOMER
ECOLIWIKI:
b1896
ECOO157CYC:
OTSA-MONOMER
INTERPRO:
IPR012766
INTERPRO:
IPR001830
MODBASE:
P31677
PDB:
2WTX
PDB:
1UQT
PDB:
1GZ5
PDB:
1UQU
PFAM:
PF00982
PRIDE:
P31677
PRODB:
PRO_000023471
REFSEQ:
NP_416410
SMR:
P31677
UNIPROT:
P31677


Operon      
Name: otsBA         
Operon arrangement:
Transcription unit        Promoter
otsA
otsBA
otsBA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2235 1982444 reverse nd [RS-EPT-CBR] [11]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Guo W., Hao H., Dai M., Wang Y., Huang L., Peng D., Wang X., Wang H., Yao M., Sun Y., Liu Z., Yuan Z., 2012, Development of quinoxaline 1, 4-dioxides resistance in Escherichia coli and molecular change under resistance selection., PLoS One 7(8):e43322

 [2] Joseph TC., Rajan LA., Thampuran N., James R., 2010, Functional characterization of trehalose biosynthesis genes from E. coli: an osmolyte involved in stress tolerance., Mol Biotechnol 46(1):20-5

 [3] Kong X., Liu Y., Gou X., Zhang H., Wang X., Zhang J., 2001, Directed evolution of operon of trehalose-6-phosphate synthase/phosphatase from Escherichia coli., Biochem Biophys Res Commun 280(1):396-400

 [4] Li H., Su H., Kim SB., Chang YK., Hong SK., Seo YG., Kim CJ., 2012, Enhanced production of trehalose in Escherichia coli by homologous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarity., J Biosci Bioeng 113(2):224-32

 [5] Nguyen Ado Q., Kim YG., Kim SB., Kim CJ., 2013, Improved tolerance of recombinant Escherichia coli to the toxicity of crude glycerol by overexpressing trehalose biosynthetic genes (otsBA) for the production of β-carotene., Bioresour Technol 143:531-7

 [6] Peng S., Stephan R., Hummerjohann J., Tasara T., 2014, Transcriptional analysis of different stress response genes in Escherichia coli strains subjected to sodium chloride and lactic acid stress., FEMS Microbiol Lett 361(2):131-7

 [7] Schultz T., Liu J., Capasso P., de Marco A., 2007, The solubility of recombinant proteins expressed in Escherichia coli is increased by otsA and otsB co-transformation., Biochem Biophys Res Commun 355(1):234-9

 [8] Stoebel DM., Dorman CJ., 2010, The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli., Mol Biol Evol 27(9):2105-12

 [9] Tondervik A., Torgersen HR., Botnmark HK., Strom AR., 2006, Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli., J Bacteriol 188(12):4218-26

 [10] Worden CR., Kovac WK., Dorn LA., Sandrin TR., 2009, Environmental pH affects transcriptional responses to cadmium toxicity in Escherichia coli K-12 (MG1655)., FEMS Microbiol Lett 293(1):58-64

 [11] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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