RegulonDB RegulonDB 10.7: Gene Form
   

otsB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

otsA araH otsB uspC otsBp otsBp TSS_2235 TSS_2235 otsBp2 otsBp2 otsAp otsAp

Gene      
Name: otsB    Texpresso search in the literature
Synonym(s): ECK1896, EG11752, b1897, otsP
Genome position(nucleotides): 1981587 <-- 1982387 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
50.56
External database links:  
ASAP:
ABE-0006320
CGSC:
18070
ECHOBASE:
EB1702
OU-MICROARRAY:
b1897
PORTECO:
otsB
REGULONDB:
b1897
STRING:
511145.b1897
M3D: otsB
COLOMBOS: otsB
PortEco: b1897


Product      
Name: trehalose-6-phosphate phosphatase
Synonym(s): OtsB, OtsP
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 29.175
Isoelectric point: 5.334
Motif(s):
 
Type Positions Sequence
18 -> 229 FFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAIARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQQGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWR
20 -> 22 DLD

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.9 - misc. glucose metabolism
  5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016787 - hydrolase activity
GO:0046872 - metal ion binding
GO:0004805 - trehalose-phosphatase activity
GO:0000287 - magnesium ion binding
biological_process GO:0006970 - response to osmotic stress
GO:0016311 - dephosphorylation
GO:0005992 - trehalose biosynthetic process
GO:0070415 - trehalose metabolism in response to cold stress
Note(s): Note(s): ...[more].
Reference(s): [1] Joseph TC., et al., 2010
[2] Kaasen I., et al., 1994
[3] Klein W., et al., 1991
[4] Kong X., et al., 2001
[5] Li H., et al., 2012
[6] Nguyen Ado Q., et al., 2013
[7] Schultz T., et al., 2007
[8] Seo HS., et al., 2000
[9] Stoebel DM., et al., 2010
[10] Tondervik A., et al., 2006
[11] Woodruff LB., et al., 2013
External database links:  
ECOCYC:
TREHALOSEPHOSPHASYN-MONOMER
ECOLIWIKI:
b1897
INTERPRO:
IPR036412
INTERPRO:
IPR023214
INTERPRO:
IPR006379
INTERPRO:
IPR003337
MODBASE:
P31678
PFAM:
PF02358
PRIDE:
P31678
PRODB:
PRO_000023472
PROTEINMODELPORTAL:
P31678
REFSEQ:
NP_416411
SMR:
P31678
UNIPROT:
P31678


Operon      
Name: otsBA         
Operon arrangement:
Transcription unit        Promoter
otsA
otsBA
otsBA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2235 1982444 reverse nd [RS-EPT-CBR] [12]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Joseph TC., Rajan LA., Thampuran N., James R., 2010, Functional characterization of trehalose biosynthesis genes from E. coli: an osmolyte involved in stress tolerance., Mol Biotechnol 46(1):20-5

 [2] Kaasen I., McDougall J., Strom AR., 1994, Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex., Gene 145(1):9-15

 [3] Klein W., Ehmann U., Boos W., 1991, The repression of trehalose transport and metabolism in Escherichia coli by high osmolarity is mediated by trehalose-6-phosphate phosphatase., Res Microbiol 142(4):359-71

 [4] Kong X., Liu Y., Gou X., Zhang H., Wang X., Zhang J., 2001, Directed evolution of operon of trehalose-6-phosphate synthase/phosphatase from Escherichia coli., Biochem Biophys Res Commun 280(1):396-400

 [5] Li H., Su H., Kim SB., Chang YK., Hong SK., Seo YG., Kim CJ., 2012, Enhanced production of trehalose in Escherichia coli by homologous expression of otsBA in the presence of the trehalase inhibitor, validamycin A, at high osmolarity., J Biosci Bioeng 113(2):224-32

 [6] Nguyen Ado Q., Kim YG., Kim SB., Kim CJ., 2013, Improved tolerance of recombinant Escherichia coli to the toxicity of crude glycerol by overexpressing trehalose biosynthetic genes (otsBA) for the production of β-carotene., Bioresour Technol 143:531-7

 [7] Schultz T., Liu J., Capasso P., de Marco A., 2007, The solubility of recombinant proteins expressed in Escherichia coli is increased by otsA and otsB co-transformation., Biochem Biophys Res Commun 355(1):234-9

 [8] Seo HS., Koo YJ., Lim JY., Song JT., Kim CH., Kim JK., Lee JS., Choi YD., 2000, Characterization of a bifunctional enzyme fusion of trehalose-6-phosphate synthetase and trehalose-6-phosphate phosphatase of Escherichia coli., Appl Environ Microbiol 66(6):2484-90

 [9] Stoebel DM., Dorman CJ., 2010, The effect of mobile element IS10 on experimental regulatory evolution in Escherichia coli., Mol Biol Evol 27(9):2105-12

 [10] Tondervik A., Torgersen HR., Botnmark HK., Strom AR., 2006, Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli., J Bacteriol 188(12):4218-26

 [11] Woodruff LB., Boyle NR., Gill RT., 2013, Engineering improved ethanol production in Escherichia coli with a genome-wide approach., Metab Eng 17:1-11

 [12] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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