RegulonDB RegulonDB 10.8: Gene Form
   

nuoM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nuoL nuoM nuoN nuoK TSS_2539 TSS_2539 TSS_2538 TSS_2538 TSS_2537 TSS_2537 TSS_2536 TSS_2536 TSS_2535 TSS_2535 TSS_2534 TSS_2534 nuoNp1 nuoNp1 TSS_2533 TSS_2533 TSS_2532 TSS_2532 TSS_2531 TSS_2531 TSS_2530 TSS_2530

Gene      
Name: nuoM    Texpresso search in the literature
Synonym(s): ECK2271, EG11773, b2277, nuoA
Genome position(nucleotides): 2391512 <-- 2393041 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.71
External database links:  
ASAP:
ABE-0007529
CGSC:
32639
ECHOBASE:
EB1722
MIM:
535000
MIM:
540000
MIM:
500001
OU-MICROARRAY:
b2277
PortEco:
nuoM
STRING:
511145.b2277
COLOMBOS: nuoM


Product      
Name: NADH:quinone oxidoreductase subunit M
Synonym(s): NADH:ubiquinone oxidoreductase, membrane subunit M, NuoA, NuoM
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 56.525
Isoelectric point: 8.682
Motif(s):
 
Type Positions Sequence
122 -> 142 WILGGVIGVFLAIDMFLFFFF
134 -> 430 IDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSGVEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLV
465 -> 485 LFMILLLVVLLVLLGFYPQPI
1 -> 21 MLLPWLILIPFIGGFLCWQTE
314 -> 334 LIAYTSVSHMGFVLIAIYTGS

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.6 - aerobic respiration
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  1 - metabolism --> 1.4 - energy production/transport --> 1.4.1 - electron donors
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.D - Oxidoreduction-driven Active Transporters
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0030964 - NADH dehydrogenase complex
GO:0045271 - respiratory chain complex I
GO:0045272 - plasma membrane respiratory chain complex I
molecular_function GO:0008137 - NADH dehydrogenase (ubiquinone) activity
GO:0048038 - quinone binding
GO:0048039 - ubiquinone binding
biological_process GO:0009060 - aerobic respiration
GO:0015990 - electron transport coupled proton transport
GO:0042773 - ATP synthesis coupled electron transport
GO:0055114 - oxidation-reduction process
Note(s): Note(s): ...[more].
Reference(s): [1] Knuuti J., et al., 2013
[2] Zhu S., et al., 2015
External database links:  
DIP:
DIP-59255N
ECOCYC:
NUOM-MONOMER
ECOLIWIKI:
b2277
INTERPRO:
IPR003918
INTERPRO:
IPR010227
INTERPRO:
IPR001750
PANTHER:
PTHR43507
PFAM:
PF00361
PRIDE:
P0AFE8
PRINTS:
PR01437
PRODB:
PRO_000023440
REFSEQ:
NP_416780
SMR:
P0AFE8
UNIPROT:
P0AFE8


Operon      
Name: nuoABCEFGHIJKLMN         
Operon arrangement:
Transcription unit        Promoter
nuoABCEFGHIJKLMN
nuoABCEFGHIJKLMN
nuoMN


Transcriptional Regulation      
Display Regulation             
Activated by: NarL, Fis
Repressed by: FNR, IHF, ArcA


Regulation by small RNA    
  Display Regulation
small RNA ryhB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2530 2390949 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2531 2391121 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2532 2391223 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2533 2391462 forward nd [RS-EPT-CBR] [3]
  promoter nuoNp1 2391717 reverse Similarity to the consensus
Read more >
[ICWHO] [4]
  promoter TSS_2534 2393605 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2535 2393635 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2536 2393749 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2537 2394474 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2538 2394595 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2539 2394680 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Knuuti J., Belevich G., Sharma V., Bloch DA., Verkhovskaya M., 2013, A single amino acid residue controls ROS production in the respiratory Complex I from Escherichia coli., Mol Microbiol 90(6):1190-200

 [2] Zhu S., Vik SB., 2015, Constraining the Lateral Helix of Respiratory Complex I by Cross-linking Does Not Impair Enzyme Activity or Proton Translocation., J Biol Chem 290(34):20761-73

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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