RegulonDB

Gene
Name:	gcvA
Synonym(s):	ECK2803, EG11795, b2808
Genome position(nucleotides):	2941650 <-- 2942567 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	49.13
External database links:

ASAP:	ABE-0009202
CGSC:	28676
ECHOBASE:	EB1743
ECOLIHUB:	gcvA
OU-MICROARRAY:	b2808
STRING:	511145.b2808
COLOMBOS:	gcvA

Shine dalgarno
Sequence:	catttaacatCAAAGTttaatagcCAT

Product

Name:

DNA-binding transcriptional dual regulator GcvA

Synonym(s):

GcvA, positive regulator of gcv operon

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

LysR

Cellular location:

cytosol

Molecular weight:

34.402

Isoelectric point:

9.311

Motif(s):

Type	Positions	Sequence
	6 -> 63	PPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLT
	8 -> 67	LNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQ
	23 -> 42	FTRAAEELFVTQAAVSHQIK
	91 -> 292	SAKGALTVSLLPSFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKA

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.1 - carbon utilization --> 1.1.3 - amino acids
		1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.17 - formyl-THF biosynthesis
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.2 - activator
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0003700 - DNA-binding transcription factor activity GO:0043565 - sequence-specific DNA binding
	biological_process	GO:0006351 - transcription, DNA-templated GO:0006355 - regulation of transcription, DNA-templated GO:0009411 - response to UV

Note(s):

Note(s): ...[more].

Evidence:

[BPP] Binding of purified proteins
[GEA] Gene expression analysis
[HIFS] Human inference of function from sequence

Reference(s):

[1] Okamura-Ikeda K., et al., 1993
[2] Sargentini NJ., et al., 2016
[3] Wilson RL., et al., 1994
[4] Wilson RL., et al., 1995

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

gcvA

Operon arrangement:

Transcription unit	Promoter
gcvA	gcvAp

Transcriptional Regulation
	Display Regulation
Repressed by:	GcvA

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	ygdDp2	2941721	reverse	nd	[ICWHO]	[5]
promoter	TSS_3118	2942729	forward	nd	[RS-EPT-CBR]	[6]
promoter	TSS_3119	2942760	forward	nd	[RS-EPT-CBR]	[6]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Okamura-Ikeda K., Ohmura Y., Fujiwara K., Motokawa Y., 1993, Cloning and nucleotide sequence of the gcv operon encoding the Escherichia coli glycine-cleavage system., Eur J Biochem 216(2):539-48
[2] Sargentini NJ., Gularte NP., Hudman DA., 2016, Screen for genes involved in radiation survival of Escherichia coli and construction of a reference database., Mutat Res 793-794:1-14
[3] Wilson RL., Stauffer GV., 1994, DNA sequence and characterization of GcvA, a LysR family regulatory protein for the Escherichia coli glycine cleavage enzyme system., J Bacteriol 176(10):2862-8
[4] Wilson RL., Urbanowski ML., Stauffer GV., 1995, DNA binding sites of the LysR-type regulator GcvA in the gcv and gcvA control regions of Escherichia coli., J Bacteriol 177(17):4940-6
[5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.