RegulonDB RegulonDB 10.8: Gene Form
   

ispF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

truD umpG ispD ispF ftsB pcm ispFp3 ispFp3 ispFp2 ispFp2 surEp2 surEp2

Gene      
Name: ispF    Texpresso search in the literature
Synonym(s): ECK2741, EG11816, b2746, ygbB
Genome position(nucleotides): 2871301 <-- 2871780 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.75
External database links:  
ASAP:
ABE-0009013
ECHOBASE:
EB1763
OU-MICROARRAY:
b2746
PortEco:
ispF
STRING:
511145.b2746
COLOMBOS: ispF


Product      
Name: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Synonym(s): IspF, YgbB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 16.898
Isoelectric point: 6.504
Motif(s):
 
Type Positions Sequence
34 -> 35 HS
131 -> 135 ATTTE
142 -> 142 R
8 -> 10 DVH
61 -> 65 FPDTD

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.11 - menaquinone, ubiquinone
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016829 - lyase activity
GO:0046872 - metal ion binding
GO:0008685 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
GO:0008270 - zinc ion binding
GO:0030145 - manganese ion binding
GO:0042802 - identical protein binding
biological_process GO:0006744 - ubiquinone biosynthetic process
GO:0008299 - isoprenoid biosynthetic process
GO:0016114 - terpenoid biosynthetic process
GO:0019288 - isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
External database links:  
DIP:
DIP-48029N
ECOCYC:
EG11816-MONOMER
ECOLIWIKI:
b2746
INTERPRO:
IPR036571
INTERPRO:
IPR020555
INTERPRO:
IPR003526
MODBASE:
P62617
PDB:
1GX1
PDB:
1KNK
PDB:
1U3L
PDB:
1U3P
PDB:
1U40
PDB:
1U43
PDB:
1YQN
PDB:
2AMT
PDB:
2GZL
PDB:
3ELC
PDB:
3EOR
PDB:
3ERN
PDB:
3ESJ
PDB:
3FBA
PDB:
1JY8
PDB:
1KNJ
PDB:
1H48
PDB:
1H47
PFAM:
PF02542
PRIDE:
P62617
PRODB:
PRO_000023038
PROSITE:
PS01350
REFSEQ:
NP_417226
SMR:
P62617
UNIPROT:
P62617


Operon      
Name: ispDF         
Operon arrangement:
Transcription unit        Promoter
ispDF


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ispFp2 2871911 reverse Similarity to the consensus
Read more >
[ICWHO] [1]
  promoter ispFp3 2871984 reverse Similarity to the consensus
Read more >
[ICWHO] [1]


Evidence    

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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