RegulonDB

Gene
Name:	yeeA
Synonym(s):	ECK2002, EG11891, b2008
Genome position(nucleotides):	2079533 <-- 2080591 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.8
External database links:

ASAP:	ABE-0006667
ECHOBASE:	EB1837
OU-MICROARRAY:	b2008
PortEco:	yeeA
STRING:	511145.b2008
COLOMBOS:	yeeA

Product

Name:

putative transporter YeeA

Synonym(s):

YeeA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

39.955

Isoelectric point:

9.78

Motif(s):

Type	Positions	Sequence
	26 -> 46	VALAFLLTFLIIRLFTIPEST
	21 -> 254	VHGTRVALAFLLTFLIIRLFTIPESTWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCAAAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTGIWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKETRIPKSIYEGIQTINRNLVCMLELQINAYWA
	69 -> 89	AFERIGGTVLGSILGLIALQL
	91 -> 111	LISLPLMLVWCAAAMFLCGWL
	47 -> 67	WPLVTMVVIMGPISFWGNVVP

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.9.B - Putative uncharacterized transport protein
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0022857 - transmembrane transporter activity
	biological_process	GO:0055085 - transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Jindal S., et al., 2019

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

yeeA

Operon arrangement:

Transcription unit	Promoter

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2312	2079341	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_2313	2079386	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_2314	2079416	reverse	nd	[RS-EPT-CBR]	[2]
promoter	yeeXp4	2079417	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.966. P-value: 5.829e-5 Read more >	[ICWHO]	[3]
promoter	TSS_2315 (cluster)	2079469	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2079468 to 2079469, where the highest frequency is at position 2077492. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[2]
promoter	yeeAp6	2080661	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 6.086. P-value: 1.526e-5 Read more >	[ICWHO]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78