RegulonDB RegulonDB 11.1: Gene Form
   

nrfF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nrfE gltP nrfD nrfG nrfF nrfC gltPp1 gltPp1 gltPp6 gltPp6 gltPp8 gltPp8

Gene      
Name: nrfF    Texpresso search in the literature
Synonym(s): ECK4068, EG11949, b4075, yjcM
Genome position(nucleotides): 4293163 --> 4293546
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.34
External database links:  
ASAP:
 ABE-0013352
CGSC:
 34361
ECHOBASE:
 EB1892
ECOLIHUB:
 nrfF
OU-MICROARRAY:
 b4075
STRING:
 511145.b4075
COLOMBOS: nrfF


Product      
Name: putative formate-dependent nitrite reductase complex subunit NrfF
Synonym(s): NrfF, YjcM, activator of formate-dependent nitrite reductase complex
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,periplasmic space
Molecular weight: 14.523
Isoelectric point: 8.887
Motif(s):
 
Type Positions Sequence Comment
1 -> 27 MNKGLLTLLLLFTCFAHAQVVDTWQFA
8 -> 126 LLLLFTCFAHAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVRAK
28 -> 127 NPQQQQQALNIASQLRCPQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQTLVLWALPVVLLLLMALILWRVRAKR UniProt: Formate-dependent nitrite reductase complex subunit NrfF.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.15 - large molecule carriers --> 1.6.15.1 - cytochromes
  2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
Gene Ontology Terms (GO)  
cellular_component GO:0042597 - periplasmic space
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
molecular_function GO:0046872 - metal ion binding
biological_process GO:0018378 - cytochrome c-heme linkage via heme-L-cysteine
Note(s): Note(s): ...[more].
Evidence: [EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Eaves DJ., et al., 1998
External database links:  
ALPHAFOLD:
 P32711
ECOCYC:
 EG11949-MONOMER
ECOLIWIKI:
 b4075
INTERPRO:
 IPR005616
INTERPRO:
 IPR017565
INTERPRO:
 IPR038297
PFAM:
 PF03918
PRIDE:
 P32711
REFSEQ:
 NP_418499
SMR:
 P32711
UNIPROT:
 P32711


Operon      
Name: nrfABCDEFG         
Operon arrangement:
Transcription unit        Promoter
nrfABCDEFG


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, IHF, NarL, FlhDC, NarP
Repressed by: NsrR, IHF, NarL, Fis


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter gltPp8 4294268 forward nd [COMP-AINF] [2]
  promoter gltPp6 4294307 forward nd [COMP-AINF] [2]
  promoter gltPp1 4294378 forward nd [COMP-AINF] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Eaves DJ., Grove J., Staudenmann W., James P., Poole RK., White SA., Griffiths I., Cole JA., 1998, Involvement of products of the nrfEFG genes in the covalent attachment of haem c to a novel cysteine-lysine motif in the cytochrome c552 nitrite reductase from Escherichia coli., Mol Microbiol 28(1):205-16

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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