RegulonDB

Gene
Name:	rfbX
Synonym(s):	ECK2031, EG11980, b2037, wzx, wzxB
Genome position(nucleotides):	2108337 <-- 2109584 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	35.42
External database links:

ASAP:	ABE-0006757
CGSC:	37353
ECHOBASE:	EB1923
OU-MICROARRAY:	b2037
PortEco:	rfbX
STRING:	511145.b2037
COLOMBOS:	rfbX

Product

Name:

polyisoprenol-linked O-antigen repeat unit flippase

Synonym(s):

RfbX, Wzx, WzxB

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

45.383

Isoelectric point:

10.292

Motif(s):

Type	Positions	Sequence
	118 -> 138	VIIWSFVPALVGNLIYPIWLF
	221 -> 241	FISTSAISLYSTGIVIILGFI
	12 -> 32	VIYLAVVQGSNYLLPLLTFPY
	84 -> 104	IFWAVIFSKIVLIVITLIFLT
	329 -> 349	IVLMIISPLPFLISLSNVYGI

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.1 - O antigen
		4 - transport --> 4.9.A - Transporters of Unknown Classification
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	biological_process	GO:0006974 - cellular response to DNA damage stimulus GO:0009103 - lipopolysaccharide biosynthetic process GO:0009243 - O antigen biosynthetic process GO:0046677 - response to antibiotic

Note(s):

Note(s): ...[more].

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

rfbBDACX

Operon arrangement:

Transcription unit	Promoter
rfbACX	rfbAp
rfbBDACX

Regulation by small RNA
	Display Regulation
small RNA	gcvB

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	rfcp8	2107234	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.46. P-value: 3.717e-5 Read more >	[ICWHO]	[1]
promoter	rfcp10	2107283	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.834. P-value: 4.678e-5 Read more >	[ICWHO]	[1]
promoter	rfcp7	2107394	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 3.395. P-value: 4.540e-5 Read more >	[ICWHO]	[1]
promoter	rfcp11	2107444	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.013. P-value: 3.013e-5 Read more >	[ICWHO]	[1]
promoter	TSS_2388	2108494	reverse	nd	[RS-EPT-CBR]	[2]
promoter	rfbXp6	2109586	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.12. P-value: 3.431e-5 Read more >	[ICWHO]	[1]
promoter	rfbXp5	2109676	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 3.715. P-value: 4.233e-5 Read more >	[ICWHO]	[1]
promoter	rfbXp8	2109716	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.876. P-value: 8.872e-5 Read more >	[ICWHO]	[1]
promoter	rfbXp7	2109718	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.63. P-value: 2.953e-5 Read more >	[ICWHO]	[1]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.