RegulonDB RegulonDB 10.8: Gene Form
   

wbbH gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rfbX wbbH glf wbbI wbbJ rfbC TSS_2388 TSS_2388 rfcp11 rfcp11 rfcp7 rfcp7 rfcp10 rfcp10 rfcp8 rfcp8 TSS_2387 TSS_2387 TSS_2386 TSS_2386 TSS_2385 TSS_2385

Gene      
Name: wbbH    Texpresso search in the literature
Synonym(s): ECK2029, EG11982, b2035, rfc, yefF
Genome position(nucleotides): 2106060 <-- 2107226 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 32.31
External database links:  
ASAP:
 ABE-0006753
ECHOBASE:
 EB1925
OU-MICROARRAY:
 b2035
PortEco:
 rfc
STRING:
 511145.b2035
COLOMBOS: wbbH


Product      
Name: putative O-antigen polymerase
Synonym(s): Rfc, WbbH, YefF
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 44.744
Isoelectric point: 9.092
Motif(s):
 
Type Positions Sequence
23 -> 43 IFYPAVCVNIIFALVLLGYEI
97 -> 117 VHNVGLLVISFSMIYICMRLS
338 -> 358 YISVKIFYSYFIYTFSFIFYH
180 -> 200 IVFIFAIILNTGKQIVFMVII
215 -> 235 VYLITAVGVLFSLYMLFLRGL

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.1 - O antigen
  6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
biological_process GO:0009103 - lipopolysaccharide biosynthetic process
Note(s): Note(s): ...[more].
Evidence: [HIFS] Human inference of function from sequence
Reference(s): [1] Stevenson G., et al., 1994
External database links:  
ECOCYC:
 EG11982-MONOMER
ECOLIWIKI:
 b2035
PRIDE:
 P37748
PRODB:
 PRO_000023731
REFSEQ:
 NP_416539
UNIPROT:
 P37748


Operon      
Name: glf-wbbH         
Operon arrangement:
Transcription unit        Promoter
glf-rfc


Regulation by small RNA    
  Display Regulation
small RNA gcvB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2385 2105457 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2386 2106618 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2387 2106661 reverse nd [RS-EPT-CBR] [2]
  promoter rfcp8 2107234 reverse Similarity to the consensus
Read more > 		[ICWHO] [3]
  promoter rfcp10 2107283 reverse Similarity to the consensus
Read more > 		[ICWHO] [3]
  promoter rfcp7 2107394 reverse Similarity to the consensus
Read more > 		[ICWHO] [3]
  promoter rfcp11 2107444 reverse Similarity to the consensus
Read more > 		[ICWHO] [3]
  promoter TSS_2388 2108494 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Stevenson G., Neal B., Liu D., Hobbs M., Packer NH., Batley M., Redmond JW., Lindquist L., Reeves P., 1994, Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster., J Bacteriol 176(13):4144-56

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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