RegulonDB RegulonDB 11.1: Gene Form
   

ccmF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ccmF ccmH ccmG ccmE ccmD ccmC ccmHp1 ccmHp1

Gene      
Name: ccmF    Texpresso search in the literature
Synonym(s): ECK2188, EG12054, b2196, yejR
Genome position(nucleotides): 2292961 <-- 2294904
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 57.1
External database links:  
ASAP:
 ABE-0007269
CGSC:
 36590
ECHOBASE:
 EB1985
ECOLIHUB:
 ccmF
OU-MICROARRAY:
 b2196
STRING:
 511145.b2196
COLOMBOS: ccmF


Product      
Name: holocytochrome c synthase CcmF component
Synonym(s): CcmF, YejR, cytochrome c heme lyase, cytochrome c maturation protein F, cytochrome c synthetase
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 71.389
Isoelectric point: 9.955
Motif(s):
 
Type Positions Sequence Comment
9 -> 29 LLCLALGIALLLSVYPLWGVA UniProt: Helical.
41 -> 63 LFAWLLFMSVAGAFLVLVNAFVV Hydrophobic region, predicted to be in the periplasm by similarity to the CcmF protein from Rhodobacter sphaeroides
42 -> 62 FAWLLFMSVAGAFLVLVNAFV UniProt: Helical.
89 -> 296 WGAHEGSLLLWVLLMSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRDLNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIFLTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWTLLLAISAFSLCLLGTFLVRS
94 -> 114 GSLLLWVLLMSGWTFAVAIFS UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.15 - large molecule carriers --> 1.6.15.1 - cytochromes
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005515 - protein binding
GO:0015232 - heme transmembrane transporter activity
GO:0020037 - heme binding
biological_process GO:0017004 - cytochrome complex assembly
GO:0015886 - heme transport
GO:0018063 - cytochrome c-heme linkage
Note(s): Note(s): ...[more].
Evidence: [EXP-IGI] Inferred from genetic interaction
[EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Ahuja U., et al., 2003
[2] San Francisco B., et al., 2014
[3] San Francisco B., et al., 2014
[4] Thony-Meyer L., et al., 1995
External database links:  
ALPHAFOLD:
 P33927
DIP:
 DIP-9256N
ECOCYC:
 EG12054-MONOMER
ECOLIWIKI:
 b2196
ECOO157CYC:
 CCMF-MONOMER
INTERPRO:
 IPR003568
INTERPRO:
 IPR003567
INTERPRO:
 IPR002541
INTERPRO:
 IPR032523
MINT:
 P33927
PFAM:
 PF01578
PFAM:
 PF16327
PRIDE:
 P33927
PRINTS:
 PR01411
PRINTS:
 PR01410
PRODB:
 PRO_000022262
REFSEQ:
 NP_416700
UNIPROT:
 P33927


Operon      
Name: napFDAGHBC-ccmABCDEFGH         
Operon arrangement:
Transcription unit        Promoter
napFDAGHBC-ccmABCDEFGH
napFDAGHBC-ccmABCDEFGH
napFDAGHBC
ccmABCDEFGH


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, ModE, FlhDC, NarP
Repressed by: NarL, NarP


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ccmHp1 2292621 reverse nd [COMP-AINF] [5]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Ahuja U., Thony-Meyer L., 2003, Dynamic features of a heme delivery system for cytochrome C maturation., J Biol Chem 278(52):52061-70

 [2] San Francisco B., Kranz RG., 2014, Interaction of holoCcmE with CcmF in heme trafficking and cytochrome c biosynthesis., J Mol Biol 426(3):570-85

 [3] San Francisco B., Sutherland MC., Kranz RG., 2014, The CcmFH complex is the system I holocytochrome c synthetase: engineering cytochrome c maturation independent of CcmABCDE., Mol Microbiol 91(5):996-1008

 [4] Thony-Meyer L., Fischer F., Kunzler P., Ritz D., Hennecke H., 1995, Escherichia coli genes required for cytochrome c maturation., J Bacteriol 177(15):4321-6

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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