RegulonDB

Gene
Name:	recX
Synonym(s):	ECK2693, EG12080, b2698, oraA
Genome position(nucleotides):	2822139 <-- 2822639 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.9
External database links:

ASAP:	ABE-0008872
ECHOBASE:	EB2005
OU-MICROARRAY:	b2698
PortEco:	recX
STRING:	511145.b2698
COLOMBOS:	recX

Product

Name:

RecA inhibitor RecX

Synonym(s):

OraA, RecX, regulatory protein RecX; inhibitor of RecA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

19.424

Isoelectric point:

8.799

Motif(s):

Type	Positions	Sequence
	50 -> 162	DATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGISREATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQEIWR
	19 -> 19	V
	120 -> 120	L

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.1 - DNA related --> 2.1.3 - DNA recombination
		2 - information transfer --> 2.1 - DNA related --> 2.1.4 - DNA repair
		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.3 - inhibition / activation of enzymes
		5 - cell processes --> 5.6 - protection --> 5.6.1 - radiation
		5 - cell processes --> 5.8 - SOS response
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0019899 - enzyme binding
	biological_process	GO:0006281 - DNA repair GO:0006974 - cellular response to DNA damage stimulus GO:0009432 - SOS response GO:0006282 - regulation of DNA repair GO:0043086 - negative regulation of catalytic activity

Note(s):

Note(s): ...[more].

Evidence:

[IDA] Inferred from direct assay

Reference(s):

[1] Bakhlanova IV., et al., 2010
[2] De Mot R., et al., 1994
[3] Dudkina AV., et al., null
[4] Long JE., et al., 2008
[5] Long JE., et al., 2009
[6] Moore JM., et al., 2012
[7] Stohl EA., et al., 2002
[8] Stohl EA., et al., 2003
[9] Venkatesh R., et al., 2002
[10] Zaitsev E., et al., 1994

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PANTHER:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

recAX

Operon arrangement:

Transcription unit	Promoter
recA	recAp
recAX	recAp

Transcriptional Regulation
	Display Regulation
Repressed by:	LexA

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_3006	2819585	reverse	nd	[RS-EPT-CBR]	[11]
promoter	TSS_3007 (cluster)	2819687	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2819686 to 2819687, where the highest frequency is at position 2817708. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[11]
promoter	TSS_3008	2821435	reverse	nd	[RS-EPT-CBR]	[11]
promoter	TSS_3009	2822024	reverse	nd	[RS-EPT-CBR]	[11]
promoter	TSS_3010	2822039	reverse	nd	[RS-EPT-CBR]	[11]
promoter	TSS_3011	2822087	reverse	nd	[RS-EPT-CBR]	[11]
promoter	TSS_3013	2823573	reverse	nd	[RS-EPT-CBR]	[11]
promoter	TSS_3014	2823575	reverse	nd	[RS-EPT-CBR]	[11]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Bakhlanova IV., Dudkina AV., Baitin DM., Knight KL., Cox MM., Lanzov VA., 2010, Modulating cellular recombination potential through alterations in RecA structure and regulation., Mol Microbiol 78(6):1523-38
[2] De Mot R., Schoofs G., Vanderleyden J., 1994, A putative regulatory gene downstream of recA is conserved in gram-negative and gram-positive bacteria., Nucleic Acids Res 22(7):1313-4
[3] Dudkina AV., Shvetsov AV., Bakhlanova IV., Baitin DM., null, [Changing of filamentation dynamics of RecA protein, induced by D112R amino acid substitution or ATP to dATP replacement, results in filament steadiness TO THE RecX protein action]., Mol Biol (Mosk) 45(3):546-53
[4] Long JE., Renzette N., Centore RC., Sandler SJ., 2008, Differential requirements of two recA mutants for constitutive SOS expression in Escherichia coli K-12., PLoS One 3(12):e4100
[5] Long JE., Renzette N., Sandler SJ., 2009, Suppression of constitutive SOS expression by recA4162 (I298V) and recA4164 (L126V) requires UvrD and RecX in Escherichia coli K-12., Mol Microbiol 73(2):226-39
[6] Moore JM., Wimberly H., Thornton PC., Rosenberg SM., Hastings PJ., 2012, Gross chromosomal rearrangement mediated by DNA replication in stressed cells: evidence from Escherichia coli., Ann N Y Acad Sci 1267:103-9
[7] Stohl EA., Blount L., Seifert HS., 2002, Differential cross-complementation patterns of Escherichia coli and Neisseria gonorrhoeae RecA proteins., Microbiology 148(Pt 6):1821-31
[8] Stohl EA., Brockman JP., Burkle KL., Morimatsu K., Kowalczykowski SC., Seifert HS., 2003, Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo., J Biol Chem 278(4):2278-85
[9] Venkatesh R., Ganesh N., Guhan N., Reddy MS., Chandrasekhar T., Muniyappa K., 2002, RecX protein abrogates ATP hydrolysis and strand exchange promoted by RecA: insights into negative regulation of homologous recombination., Proc Natl Acad Sci U S A 99(19):12091-6
[10] Zaitsev E., Alexseyev A., Lanzov V., Satin L., Clark AJ., 1994, Nucleotide sequence between recA and alaSp in E. coli K12 and the sequence change in four recA mutations., Mutat Res 323(4):173-7
[11] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.