RegulonDB RegulonDB 10.8: Gene Form
   

recX gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

alaS recA recX AlaS anti-anti-terminator anti-terminator terminator TSS_3014 TSS_3014 TSS_3013 TSS_3013 alaSp alaSp TSS_3011 TSS_3011 TSS_3010 TSS_3010 TSS_3009 TSS_3009 TSS_3008 TSS_3008 TSS_3007 (cluster) TSS_3007 (cluster) TSS_3006 TSS_3006

Gene      
Name: recX    Texpresso search in the literature
Synonym(s): ECK2693, EG12080, b2698, oraA
Genome position(nucleotides): 2822139 <-- 2822639 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.9
External database links:  
ASAP:
ABE-0008872
ECHOBASE:
EB2005
OU-MICROARRAY:
b2698
PortEco:
recX
STRING:
511145.b2698
COLOMBOS: recX


Product      
Name: RecA inhibitor RecX
Synonym(s): OraA, RecX, regulatory protein RecX; inhibitor of RecA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 19.424
Isoelectric point: 8.799
Motif(s):
 
Type Positions Sequence
50 -> 162 DATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGISREATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQEIWR
19 -> 19 V
120 -> 120 L

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.1 - DNA related --> 2.1.3 - DNA recombination
  2 - information transfer --> 2.1 - DNA related --> 2.1.4 - DNA repair
  3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.3 - inhibition / activation of enzymes
  5 - cell processes --> 5.6 - protection --> 5.6.1 - radiation
  5 - cell processes --> 5.8 - SOS response
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0019899 - enzyme binding
biological_process GO:0006281 - DNA repair
GO:0006974 - cellular response to DNA damage stimulus
GO:0009432 - SOS response
GO:0006282 - regulation of DNA repair
GO:0043086 - negative regulation of catalytic activity
Note(s): Note(s): ...[more].
Evidence: [IDA] Inferred from direct assay
Reference(s): [1] Bakhlanova IV., et al., 2010
[2] De Mot R., et al., 1994
[3] Dudkina AV., et al., null
[4] Long JE., et al., 2008
[5] Long JE., et al., 2009
[6] Moore JM., et al., 2012
[7] Stohl EA., et al., 2002
[8] Stohl EA., et al., 2003
[9] Venkatesh R., et al., 2002
[10] Zaitsev E., et al., 1994
External database links:  
DIP:
DIP-10410N
ECOCYC:
EG12080-MONOMER
ECOLIWIKI:
b2698
INTERPRO:
IPR036388
INTERPRO:
IPR003783
PANTHER:
PTHR33602
PDB:
2A7Z
PFAM:
PF02631
PRIDE:
P33596
PRODB:
PRO_000023709
REFSEQ:
NP_417178
SMR:
P33596
UNIPROT:
P33596


Operon      
Name: recAX         
Operon arrangement:
Transcription unit        Promoter
recA
recAX


Transcriptional Regulation      
Display Regulation             
Repressed by: LexA


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3006 2819585 reverse nd [RS-EPT-CBR] [11]
  promoter TSS_3007 (cluster) 2819687 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [11]
  promoter TSS_3008 2821435 reverse nd [RS-EPT-CBR] [11]
  promoter TSS_3009 2822024 reverse nd [RS-EPT-CBR] [11]
  promoter TSS_3010 2822039 reverse nd [RS-EPT-CBR] [11]
  promoter TSS_3011 2822087 reverse nd [RS-EPT-CBR] [11]
  promoter TSS_3013 2823573 reverse nd [RS-EPT-CBR] [11]
  promoter TSS_3014 2823575 reverse nd [RS-EPT-CBR] [11]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Bakhlanova IV., Dudkina AV., Baitin DM., Knight KL., Cox MM., Lanzov VA., 2010, Modulating cellular recombination potential through alterations in RecA structure and regulation., Mol Microbiol 78(6):1523-38

 [2] De Mot R., Schoofs G., Vanderleyden J., 1994, A putative regulatory gene downstream of recA is conserved in gram-negative and gram-positive bacteria., Nucleic Acids Res 22(7):1313-4

 [3] Dudkina AV., Shvetsov AV., Bakhlanova IV., Baitin DM., null, [Changing of filamentation dynamics of RecA protein, induced by D112R amino acid substitution or ATP to dATP replacement, results in filament steadiness TO THE RecX protein action]., Mol Biol (Mosk) 45(3):546-53

 [4] Long JE., Renzette N., Centore RC., Sandler SJ., 2008, Differential requirements of two recA mutants for constitutive SOS expression in Escherichia coli K-12., PLoS One 3(12):e4100

 [5] Long JE., Renzette N., Sandler SJ., 2009, Suppression of constitutive SOS expression by recA4162 (I298V) and recA4164 (L126V) requires UvrD and RecX in Escherichia coli K-12., Mol Microbiol 73(2):226-39

 [6] Moore JM., Wimberly H., Thornton PC., Rosenberg SM., Hastings PJ., 2012, Gross chromosomal rearrangement mediated by DNA replication in stressed cells: evidence from Escherichia coli., Ann N Y Acad Sci 1267:103-9

 [7] Stohl EA., Blount L., Seifert HS., 2002, Differential cross-complementation patterns of Escherichia coli and Neisseria gonorrhoeae RecA proteins., Microbiology 148(Pt 6):1821-31

 [8] Stohl EA., Brockman JP., Burkle KL., Morimatsu K., Kowalczykowski SC., Seifert HS., 2003, Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo., J Biol Chem 278(4):2278-85

 [9] Venkatesh R., Ganesh N., Guhan N., Reddy MS., Chandrasekhar T., Muniyappa K., 2002, RecX protein abrogates ATP hydrolysis and strand exchange promoted by RecA: insights into negative regulation of homologous recombination., Proc Natl Acad Sci U S A 99(19):12091-6

 [10] Zaitsev E., Alexseyev A., Lanzov V., Satin L., Clark AJ., 1994, Nucleotide sequence between recA and alaSp in E. coli K12 and the sequence change in four recA mutations., Mutat Res 323(4):173-7

 [11] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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