RegulonDB

Gene
Name:	acrR
Synonym(s):	ECK0458, EG12116, b0464, ybaH
Genome position(nucleotides):	485761 --> 486408 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	45.37
External database links:

ASAP:	ABE-0001608
CGSC:	35809
ECHOBASE:	EB2039
OU-MICROARRAY:	b0464
PortEco:	acrR
STRING:	511145.b0464
COLOMBOS:	acrR

Product

Name:

DNA-binding transcriptional repressor AcrR

Synonym(s):

AcrR, YbaH

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

TetR/AcrR

Cellular location:

cytosol

Molecular weight:

24.767

Isoelectric point:

5.703

Motif(s):

Type	Positions	Sequence
	16 -> 62	ILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI
	29 -> 29	V
	33 -> 52	SLGEIAKAAGVTRGAIYWHF
	84 -> 202	DPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMY
	10 -> 70	QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNI

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
		5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding GO:0003700 - DNA-binding transcription factor activity GO:0043565 - sequence-specific DNA binding GO:0008144 - drug binding GO:0000976 - transcription regulatory region sequence-specific DNA binding
	biological_process	GO:0006351 - transcription, DNA-templated GO:0006355 - regulation of transcription, DNA-templated GO:0042493 - response to drug GO:0045892 - negative regulation of transcription, DNA-templated

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[GEA] Gene expression analysis
[IEP] Inferred from expression pattern
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Ma D., et al., 1996

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PDB:

PDB:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMR:

SWISSMODEL:

UNIPROT:

Operon

Name:

acrR

Operon arrangement:

Transcription unit	Promoter
acrR	acrRp

Transcriptional Regulation
	Display Regulation
Repressed by:	AcrR

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_623 (cluster)	485648	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 485647 to 485648, where the highest frequency is at position 484872. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[2]
promoter	TSS_624	485663	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_625	485716	forward	nd	[RS-EPT-CBR]	[2]
promoter	TSS_626 (cluster)	485980	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 485979 to 485980, where the highest frequency is at position 485204. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[2]
promoter	TSS_627	486041	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_628	486086	reverse	nd	[RS-EPT-CBR]	[2]
promoter	TSS_629	487911	forward	nd	[RS-EPT-CBR]	[2]
promoter	TSS_630	488317	forward	nd	[RS-EPT-CBR]	[2]
promoter	TSS_631	489636	forward	nd	[RS-EPT-CBR]	[2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Ma D., Alberti M., Lynch C., Nikaido H., Hearst JE., 1996, The local repressor AcrR plays a modulating role in the regulation of acrAB genes of Escherichia coli by global stress signals., Mol Microbiol 19(1):101-12
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Ma D., Alberti M., Lynch C., Nikaido H., Hearst JE., 1996, The local repressor AcrR plays a modulating role in the regulation of acrAB genes of Escherichia coli by global stress signals., Mol Microbiol 19(1):101-12

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.