RegulonDB

Gene
Name:	acrR
Synonym(s):	ECK0458, EG12116, b0464, ybaH
Genome position(nucleotides):	485761 --> 486408 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	45.37
External database links:

ASAP:	ABE-0001608
CGSC:	35809
ECHOBASE:	EB2039
ECOLIHUB:	acrR
OU-MICROARRAY:	b0464
STRING:	511145.b0464
COLOMBOS:	acrR

Product

Name:

DNA-binding transcriptional repressor AcrR

Synonym(s):

AcrR, YbaH

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

TetR/AcrR

Cellular location:

cytosol

Molecular weight:

24.767

Isoelectric point:

5.703

Motif(s):

Type	Positions	Sequence
	16 -> 62	ILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI
	29 -> 29	V
	33 -> 52	SLGEIAKAAGVTRGAIYWHF
	84 -> 202	DPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMY
	10 -> 70	QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNI

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
		5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding GO:0015643 - toxic substance binding GO:0003700 - DNA-binding transcription factor activity GO:0043565 - sequence-specific DNA binding GO:0000976 - transcription cis-regulatory region binding GO:0000984 - bacterial-type RNA polymerase transcription regulatory region sequence-specific DNA binding
	biological_process	GO:0006355 - regulation of transcription, DNA-templated GO:0009410 - response to xenobiotic stimulus GO:0045892 - negative regulation of transcription, DNA-templated

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[GEA] Gene expression analysis
[IEP] Inferred from expression pattern
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Gu R., et al., 2008
[2] Ma D., et al., 1996

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PDB:

PDB:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMR:

SWISSMODEL:

UNIPROT:

Operon

Name:

acrR

Operon arrangement:

Transcription unit	Promoter
acrR	acrRp

Transcriptional Regulation
	Display Regulation
Repressed by:	AcrR

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_623 (cluster)	485648	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_624	485663	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_625	485716	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_626 (cluster)	485980	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_627	486041	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_628	486086	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_629	487911	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_630	488317	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_631	489636	forward	nd	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Gu R., Li M., Su CC., Long F., Routh MD., Yang F., McDermott G., Yu EW., 2008, Conformational change of the AcrR regulator reveals a possible mechanism of induction., Acta Crystallogr Sect F Struct Biol Cryst Commun 64(Pt 7):584-8
[2] Ma D., Alberti M., Lynch C., Nikaido H., Hearst JE., 1996, The local repressor AcrR plays a modulating role in the regulation of acrAB genes of Escherichia coli by global stress signals., Mol Microbiol 19(1):101-12
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.