RegulonDB

Gene
Name:	glnK
Synonym(s):	ECK0444, EG12191, b0450, ybaI
Genome position(nucleotides):	472598 --> 472936 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.74
External database links:

ASAP:	ABE-0001562
ECHOBASE:	EB2108
OU-MICROARRAY:	b0450
PORTECO:	glnK
REGULONDB:	b0450
STRING:	511145.b0450
M3D:	glnK
COLOMBOS:	glnK
PortEco:	b0450

Shine dalgarno
Sequence:	aattctgaccGGAGGGgatctATG

Product

Name:

nitrogen regulator GlnK

Synonym(s):

GlnK, PII2, YbaI, [protein PII2], nitrogen regulator PII 2, nitrogen regulatory protein GlnK, unmodified protein PII 2

Sequence:

Get amino acid sequence Fasta Format

Molecular weight:

12.259

Isoelectric point:

5.919

Motif(s):

Type	Positions	Sequence
	4 -> 105	VTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAELYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRIRTG

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.8 - metabolism of other compounds --> 1.8.3 - nitrogen metabolism
		3 - regulation --> 3.1 - type of regulation --> 3.1.4 - unknown
Gene Ontology Terms (GO)
	molecular_function	GO:0005515 - protein binding GO:0030234 - enzyme regulator activity GO:0000166 - nucleotide binding GO:0042802 - identical protein binding
	biological_process	GO:0050790 - regulation of catalytic activity GO:0006808 - regulation of nitrogen utilization GO:0045848 - positive regulation of nitrogen utilization

Reference(s):

[1] van Heeswijk WC., et al., 1996

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MINT:

MODBASE:

PANTHER:

PDB:

PDB:

PDB:

PDB:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

PROSITE:

PROTEINMODELPORTAL:

REFSEQ:

SMART:

SMR:

UNIPROT:

Operon

Name:

glnK-amtB

Operon arrangement:

Transcription unit	Promoter
glnK-amtB	glnKp

Transcriptional Regulation
	Display Regulation
Activated by:	GadX, Fur, FNR, NtrC
Repressed by:	Fur, FNR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_609	472557		forward	nd	[RS-EPT-CBR]	[2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] van Heeswijk WC., Hoving S., Molenaar D., Stegeman B., Kahn D., Westerhoff HV., 1996, An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli., Mol Microbiol 21(1):133-46
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] van Heeswijk WC., Hoving S., Molenaar D., Stegeman B., Kahn D., Westerhoff HV., 1996, An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli., Mol Microbiol 21(1):133-46

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.