RegulonDB RegulonDB 11.1: Gene Form
   

mdtE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mdtF mdtE gadF TSS_4128 TSS_4128 mdtEp3 mdtEp3 mdtEp2 mdtEp2 mdtEp1 mdtEp1

Gene      
Name: mdtE    Texpresso search in the literature
Synonym(s): ECK3497, EG12240, b3513, yhiU
Genome position(nucleotides): 3659232 --> 3660389
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.58
External database links:  
ASAP:
ABE-0011478
ECHOBASE:
EB2151
ECOLIHUB:
mdtE
OU-MICROARRAY:
b3513
STRING:
511145.b3513
COLOMBOS: mdtE


Product      
Name: multidrug efflux pump membrane fusion protein MdtE
Synonym(s): MdtE, YhiU
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane,periplasmic space
Molecular weight: 41.191
Isoelectric point: 5.563
Motif(s):
 
Type Positions Sequence Comment
1 -> 20 MNRRRKLLIPLLFCGAMLTA
21 -> 385 CDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ UniProt: Multidrug resistance protein MdtE.
35 -> 356 EVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVI
60 -> 289 EVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYV

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.8.A - Accessory Factors Involved in Transport
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0031226 - intrinsic component of plasma membrane
molecular_function GO:0015125 - bile acid transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
GO:0042802 - identical protein binding
biological_process GO:0009636 - response to toxic substance
GO:0046677 - response to antibiotic
GO:0055085 - transmembrane transport
GO:0042908 - xenobiotic transport
GO:0015721 - bile acid and bile salt transport
Note(s): Note(s): ...[more].
Reference(s): [1] Elkins CA., et al., 2003
[2] Jindal S., et al., 2019
[3] Rao S., et al., 2020
External database links:  
ALPHAFOLD:
P37636
ECOCYC:
EG12240-MONOMER
ECOLIWIKI:
b3513
INTERPRO:
IPR043602
INTERPRO:
IPR006143
INTERPRO:
IPR032317
MODBASE:
P37636
PFAM:
PF00529
PFAM:
PF16576
PRIDE:
P37636
PRODB:
PRO_000023185
PROSITE:
PS51257
REFSEQ:
NP_417970
SMR:
P37636
SWISSMODEL:
P37636
UNIPROT:
P37636


Operon      
Name: gadEF-mdtEF         
Operon arrangement:
Transcription unit        Promoter
gadEF
gadE-mdtEF
gadEF
gadEF-mdtEF
mdtEF
mdtEF
mdtEF


Transcriptional Regulation      
Display Regulation             
Activated by: EvgA, GadX, YdeO, GadW, GadE
Repressed by: FliZ, CRP, H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4128 3659216 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Elkins CA., Nikaido H., 2003, Chimeric analysis of AcrA function reveals the importance of its C-terminal domain in its interaction with the AcrB multidrug efflux pump., J Bacteriol 185(18):5349-56

 [2] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [3] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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