RegulonDB RegulonDB 10.8: Gene Form
   

mdtE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mdtF mdtE gadF TSS_4128 TSS_4128 mdtEp3 mdtEp3 mdtEp2 mdtEp2 mdtEp1 mdtEp1

Gene      
Name: mdtE    Texpresso search in the literature
Synonym(s): ECK3497, EG12240, b3513, yhiU
Genome position(nucleotides): 3659232 --> 3660389 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.58
External database links:  
ASAP:
 ABE-0011478
ECHOBASE:
 EB2151
OU-MICROARRAY:
 b3513
PortEco:
 mdtE
STRING:
 511145.b3513
COLOMBOS: mdtE


Product      
Name: multidrug efflux pump membrane fusion protein MdtE
Synonym(s): MdtE, YhiU
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space,inner membrane
Molecular weight: 41.191
Isoelectric point: 5.563
Motif(s):
 
Type Positions Sequence
35 -> 356 EVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVI
21 -> 385 CDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ
60 -> 289 EVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYV
1 -> 20 MNRRRKLLIPLLFCGAMLTA

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.8.A - Accessory Factors Involved in Transport
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0005886 - plasma membrane
GO:0031226 - intrinsic component of plasma membrane
molecular_function GO:0015125 - bile acid transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
GO:0042802 - identical protein binding
GO:0042910 - xenobiotic transmembrane transporter activity
biological_process GO:0009636 - response to toxic substance
GO:0046677 - response to antibiotic
GO:0055085 - transmembrane transport
GO:0042908 - xenobiotic transport
GO:0015721 - bile acid and bile salt transport
Note(s): Note(s): ...[more].
Reference(s): [1] Elkins CA., et al., 2003
[2] Jindal S., et al., 2019
External database links:  
ECOCYC:
 EG12240-MONOMER
ECOLIWIKI:
 b3513
INTERPRO:
 IPR032317
INTERPRO:
 IPR006143
MODBASE:
 P37636
PFAM:
 PF00529
PFAM:
 PF16576
PRIDE:
 P37636
PRODB:
 PRO_000023185
PROSITE:
 PS51257
REFSEQ:
 NP_417970
SMR:
 P37636
SWISSMODEL:
 P37636
UNIPROT:
 P37636


Operon      
Name: gadEF-mdtEF         
Operon arrangement:
Transcription unit        Promoter
gadE
gadE-mdtEF
gadE
gadEF-mdtEF
mdtEF
mdtEF
mdtEF


Transcriptional Regulation      
Display Regulation             
Activated by: EvgA, GadX, GadW, YdeO, GadE
Repressed by: CRP, FliZ, H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4128 3659216 forward nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Elkins CA., Nikaido H., 2003, Chimeric analysis of AcrA function reveals the importance of its C-terminal domain in its interaction with the AcrB multidrug efflux pump., J Bacteriol 185(18):5349-56

 [2] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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